M
Mingzhi Zhao
Researcher at Protein Sciences
Publications - 13
Citations - 193
Mingzhi Zhao is an academic researcher from Protein Sciences. The author has contributed to research in topics: Trypsin & Proteome. The author has an hindex of 8, co-authored 12 publications receiving 141 citations.
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Journal ArticleDOI
Enhanced Purification of Ubiquitinated Proteins by Engineered Tandem Hybrid Ubiquitin-binding Domains (ThUBDs)
Yuan Gao,Yanchang Li,Chengpu Zhang,Mingzhi Zhao,Chen Deng,Qiuyan Lan,Zexian Liu,Na Su,Jingwei Wang,Feng Xu,Yongru Xu,Lingyan Ping,Lei Chang,Gao Huiying,Junzhu Wu,Yu Xue,Zixin Deng,Junmin Peng,Ping Xu +18 more
TL;DR: ThUBD is a refined and promising approach for enriching the ubiquitinated proteome while circumventing the need to overexpress tagged ubiquitin variants and use antibodies to recognize ubiquitIn remnants, thus providing a readily accessible tool for the protein ubiquitination research community.
Journal ArticleDOI
Quantitative Proteomics Reveals Membrane Protein-Mediated Hypersaline Sensitivity and Adaptation in Halophilic Nocardiopsis xinjiangensis.
Yao Zhang,Yao Zhang,Yanchang Li,Yongguang Zhang,Zhi Qiang Wang,Zhi Qiang Wang,Mingzhi Zhao,Na Su,Tao Zhang,Lingsheng Chen,Lingsheng Chen,Wei Wei,Jing Luo,Jing Luo,Yanxia Zhou,Yanxia Zhou,Yongru Xu,Yongru Xu,Ping Xu,Ping Xu,Ping Xu,Wen-Jun Li,Wen-Jun Li,Wen-Jun Li,Yong Tao +24 more
TL;DR: Overall, regulation of membrane proteins may provide a major protection strategy against hyperosmotic stress.
Journal ArticleDOI
Precision De Novo Peptide Sequencing Using Mirror Proteases of Ac-LysargiNase and Trypsin for Large-scale Proteomics.
Hao Yang,Yanchang Li,Mingzhi Zhao,Feilin Wu,Xi Wang,Weidi Xiao,Yi-Hao Wang,Junling Zhang,Fuqiang Wang,Feng Xu,Wen-Feng Zeng,Christopher M. Overall,Si-Min He,Hao Chi,Ping Xu,Ping Xu,Ping Xu +16 more
TL;DR: The developed acetylated LysargiNase, with superior activity and stability, provides complementary ion types compared with trypsin for MS/MS analysis and a novel de novo sequencing algorithm, pNovoM, which performed with higher efficiency and accuracy compared with other software tools.
Journal ArticleDOI
Development of a rapid high-efficiency scalable process for acetylated Sus scrofa cationic trypsin production from Escherichia coli inclusion bodies
TL;DR: This is the first report of production of acetylated recombinant trypsin that is stable and suitable for scale-up and higher BAEE activity than a commercial product (9500 BAEE unit/mg, Promega).
Journal ArticleDOI
Recombinant acetylated trypsin demonstrates superior stability and higher activity than commercial products in quantitative proteomics studies.
Feilin Wu,Feilin Wu,Mingzhi Zhao,Yao Zhang,Yao Zhang,Na Su,Zhi Xiong,Ping Xu,Ping Xu,Ping Xu +9 more
TL;DR: The newly developed r-Ac-trypsin was more resistant to autolysis, which enabled more complete digestion of proteomic samples and showed similar or superior properties such as stability activity and specificity to commercial products.