Mitchell R. O’Connell

TL;DR: It is shown that bacterial C2c2 possesses a unique RNase activity responsible for CRISPR RNA maturation that is distinct from its RNA-activated single-stranded RNA degradation activity.

TL;DR: It is shown that Cas9 binds with high affinity to single-stranded RNA targets matching the Cas9-associated guide RNA sequence when the PAM is presented in trans as a separate DNA oligonucleotide, revealing a fundamental connection between PAM binding and substrate selection by Cas9 and highlighting the utility of Cas9 for programmable transcript recognition without the need for tags.

TL;DR: It is demonstrated that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and allow endogenous RNA tracking in living cells and establishes RCas9 as a means to track RNA inliving cells in a programmable manner without genetically encoded tags.

TL;DR: It is found that Cas13a pre-crRNA processing is not essential for ssRNA cleavage, although it enhances ssRNA targeting for crRNAs encoded internally within the CRISPR array.

TL;DR: This review will compare and contrast what is known about the molecular architecture and behavior of Type VI (A-D) CRISPR-Cas13 interference complexes, how this allows them to carry out their RNA-targeting function, how Type VI accessory proteins are able to modulate Cas13 activity, and how together all of these features have led to the rapid development of a range of RNA- targeting applications.