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Nancy Kleckner
Researcher at Harvard University
Publications - 223
Citations - 37095
Nancy Kleckner is an academic researcher from Harvard University. The author has contributed to research in topics: Tn10 & Meiosis. The author has an hindex of 92, co-authored 217 publications receiving 34993 citations. Previous affiliations of Nancy Kleckner include Massachusetts Institute of Technology & University of California, Los Angeles.
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Capturing Chromosome Conformation
TL;DR: Using the yeast Saccharomyces cerevisiae, this work could confirm known qualitative features of chromosome organization within the nucleus and dynamic changes in that organization during meiosis and found that chromatin is highly flexible throughout.
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Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family.
TL;DR: These findings strongly implicate Spo11 as the catalytic subunit of the meiotic DNA cleavage activity and provide direct evidence that the mechanism of meiotic recombination initiation is evolutionarily conserved.
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Improved single and multicopy lac-based cloning vectors for protein and operon fusions
TL;DR: Several new vectors for the construction of operon and protein fusions to the Escherichia coli lacZ gene are described, improved in that they have very low levels of background lac gene expression, which makes possible the easy detection and accurate quantitation of very weak transcriptional and translational signals.
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Meiotic chromosomes: integrating structure and function.
Denise Zickler,Nancy Kleckner +1 more
TL;DR: The current article reviews recent information on diverse aspects of chromosome morphogenesis, notably relationships between sisters, development of axial structure, and variations in chromatin status in an historical context.
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DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
TL;DR: DMC1 phenotypes provide further evidence that recombination and SC formation are interrelated processes and are consistent with a requirement for DNA-DNA interactions during SC formation, and additional evidence suggests that arrest occurs at a meiosis-specific cell cycle "checkpoint" in response to a primary defect in prophase chromosome metabolism.