Neil C. Sheppard

TL;DR: Polyethyleneimine (PEI) represents a family of organic polycations used as nucleic acid transfection reagents in vitro and DNA vaccine delivery vehicles in vivo and it is shown that diverse PEI forms have potent mucosal adjuvant activity for viral subunit glycoprotein antigens.

TL;DR: It is suggested that non-sialic-acid-imparting systems yield gp120 immunogens with modified antigenic and immunogenic properties, considerations that should be considered when selecting expression systems for glycosylated antigens to be used for structure-function studies and for vaccine use.

TL;DR: This study establishes a rationale for eliminating HIV-1-infected cells by targeting cellular immune responses against stable human endogenous retroviral (HERV) antigens and identifies a second T cell response that exhibited cross-reactivity between homologous HIV- 1-Pol and HERV-K(HML-2)-Pol determinants, raising the possibility that homology between HIV-2 and HERVs plays a role in shaping, and perhaps enhancing, the T cellresponse to HIV

TL;DR: NYVAC-C (vP2010), a recombinant vector expressing HIV subtype C gag, pol, env and nef antigens, was tested in a phase I study in healthy, HIV negative volunteers in London and Lausanne and found no serious adverse events, and no clinical or laboratory abnormalities or other events that led to withdrawal, interruption or dose reduction of the NYVAC/placebo.

TL;DR: The results indicate that the TRIM5 alleles can be a barrier to productive infection and that this should be taken into account when designing acquisition studies using SIVsmE660 or related viruses.