Showing papers by "Qingming Luo published in 2022"

Acetylcholine deficiency disrupts extratelencephalic projection neurons in the prefrontal cortex in a mouse model of Alzheimer’s disease

Abstract: Short-term memory deficits have been associated with prefrontal cortex (PFC) dysfunction in Alzheimer's disease (AD) and AD mouse models. Extratelencephalic projection (ET) neurons in the PFC play a key role in short-term working memory, but the mechanism between ET neuronal dysfunction in the PFC and short-term memory impairment in AD is not well understood. Here, using fiber photometry and optogenetics, we found reduced neural activity in the ET neurons in the medial prefrontal cortex (mPFC) of the 5×FAD mouse model led to object recognition memory (ORM) deficits. Activation of ET neurons in the mPFC of 5×FAD mice rescued ORM impairment, and inhibition of ET neurons in the mPFC of wild type mice impaired ORM expression. ET neurons in the mPFC that project to supramammillary nucleus were necessary for ORM expression. Viral tracing and in vivo recording revealed that mPFC ET neurons received fewer cholinergic inputs from the basal forebrain in 5×FAD mice. Furthermore, activation of cholinergic fibers in the mPFC rescued ORM deficits in 5×FAD mice, while acetylcholine deficiency reduced the response of ET neurons in the mPFC to familiar objects. Taken together, our results revealed a neural mechanism behind ORM impairment in 5×FAD mice.

Wideband Back-Cover Antenna Design Using Dual Characteristic Modes With High Isolation for 5G MIMO Smartphone

Abstract: A novel method of designing a wideband high-isolated dual-antenna pair using dual characteristic modes (CMs) is presented for fifth-generation (5G) multiple-input multiple-output (MIMO) smartphone applications. A set of orthogonal CMs resonating from the square-loop slot is first introduced and works for the lower band. Then, another set of orthogonal CMs resonating from the edge branches is introduced with a shared compact radiator and works for the higher band. In combination with two sets of degenerated CMs and a capacitive coupling feeding structure, the proposed dual-antenna pair achieves a broad impedance bandwidth and high isolation without the need for any external decoupling structures. Based on this dual-antenna pair, an 8×8 MIMO array is developed and integrated into the back cover of a smartphone, which realizes zero ground clearance on the system circuit board. To verify the design concept, prototypes of the antenna pair and MIMO array were fabricated and measured. It shows that experimental results agree well with the simulation results. More importantly, the presented 8×8 MIMO array has high isolation of more than 20 dB is achieved across the operating band of 3.3-3.8 GHz.

Large depth-of-field fluorescence microscopy based on deep learning supported by Fresnel incoherent correlation holography.

Abstract: Fluorescence microscopy plays an irreplaceable role in biomedicine. However, limited depth of field (DoF) of fluorescence microscopy is always an obstacle of image quality, especially when the sample is with an uneven surface or distributed in different depths. In this manuscript, we combine deep learning with Fresnel incoherent correlation holography to describe a method to obtain significant large DoF fluorescence microscopy. Firstly, the hologram is restored by the Auto-ASP method from out-of-focus to in-focus in double-spherical wave Fresnel incoherent correlation holography. Then, we use a generative adversarial network to eliminate the artifacts introduced by Auto-ASP and output the high-quality image as a result. We use fluorescent beads, USAF target and mouse brain as samples to demonstrate the large DoF of more than 400µm, which is 13 times better than that of traditional wide-field microscopy. Moreover, our method is with a simple structure, which can be easily combined with many existing fluorescence microscopic imaging technology.

Multiscale reconstruction of various vessels in the intact murine liver lobe

Abstract: The liver contains a variety of vessels and participates in miscellaneous physiological functions. While past studies generally focused on certain hepatic vessels, we simultaneously obtained all the vessels and cytoarchitectural information of the intact mouse liver lobe at single-cell resolution. Here, taking structural discrepancies of various vessels into account, we reconstruct and visualize the portal vein, hepatic vein, hepatic artery, intrahepatic bile duct, intrahepatic lymph of an intact liver lobe and peribiliary plexus in its selected local areas, providing a technology roadmap for studying the fine hepatic vascular structures and their spatial relationship, which will help research into liver diseases and evaluation of medical efficacies in the future.

Extension of Endocardium-Derived Vessels Generate Coronary Arteries in Neonates

Abstract: Supplemental Digital Content is available in the text. Background: Unraveling how new coronary arteries develop may provide critical information for establishing novel therapeutic approaches to treating ischemic cardiac diseases. There are 2 distinct coronary vascular populations derived from different origins in the developing heart. Understanding the formation of coronary arteries may provide insights into new ways of promoting coronary artery formation after myocardial infarction. Methods: To understand how intramyocardial coronary arteries are generated to connect these 2 coronary vascular populations, we combined genetic lineage tracing, light sheet microscopy, fluorescence micro-optical sectioning tomography, and tissue-specific gene knockout approaches to understand their cellular and molecular mechanisms. Results: We show that a subset of intramyocardial coronary arteries form by angiogenic extension of endocardium-derived vascular tunnels in the neonatal heart. Three-dimensional whole-mount fluorescence imaging showed that these endocardium-derived vascular tunnels or tubes adopt an arterial fate in neonates. Mechanistically, we implicate Mettl3 (methyltransferase-like protein 3) and Notch signaling in regulating endocardium-derived intramyocardial coronary artery formation. Functionally, these intramyocardial arteries persist into adulthood and play a protective role after myocardial infarction. Conclusions: A subset of intramyocardial coronary arteries form by extension of endocardium-derived vascular tunnels in the neonatal heart.

A Compact Dual-Polarized Filtering Antenna with Steep Cut-Off for Base-Station Applications

Abstract: A dual-polarized filtering antenna with steep cut-off and compact size is developed for base station applications. In this design, four controllable radiation nulls are obtained by utilizing split rings, slotted T-shaped branches, a single-stub tuner, and a parasitic loop. Split rings are firstly used as the dipole arms to obtain the 1st radiation null at upper out-of-band. Four T-shaped branches working as DGS are printed under the crossed dipoles to achieve the 2nd radiation null. The connected outer conductors of the differential feed structure acting as a single-stub tuner can provide the 3rd radiation null to further enhance the upper-band rejection. Finally, a parasitic loop is incorporated around the split rings, and the out-of-band rejection of the lower-band is further enhanced by the 4th radiation null. More importantly, the impedance bandwidth of the antenna can be expended with two newly introduced resonant modes. As a result, a compact filtering antenna with a wide operational bandwidth of 1.7- 3.01 GHz (56%) is realized for |Sdd11| < -15 dB with the isolation higher than 38 dB. The out-of-band suppression is higher than 18.4 dB in 3.1-4.5 GHz and more than 47 dB in 0.8-1.1 GHz.

Interpretable model-driven projected gradient descent network for high-quality fDOT reconstruction.

Abstract: In fluorescence diffuse optical tomography (fDOT), the quality of reconstruction is severely limited by mismodeling and ill-posedness of inverse problems. Although data-driven deep learning methods improve the quality of image reconstruction, the network architecture lacks interpretability and requires a lot of data for training. We propose an interpretable model-driven projected gradient descent network (MPGD-Net) to improve the quality of fDOT reconstruction using only a few training samples. MPGD-Net unfolds projected gradient descent into a novel deep network architecture that is naturally interpretable. Simulation and in vivo experiments show that MPGD-Net greatly improves the fDOT reconstruction quality with superior generalization ability.

Mesoscale visualization of three-dimensional microvascular architecture and immunocyte distribution in intact mouse liver lobes

Abstract: Rational: The complex vascular architecture and diverse immune cells of the liver are critical for maintaining liver homeostasis. However, quantification of the network of liver vasculature and immunocytes at different scales from a single hepatic lobule to an intact liver lobe remains challenging. Methods: Here, we developed a fast and fluorescence-preserving transparency method, denoted liver-CUBIC, for systematic and integrated analysis of the microcirculation and the three-dimensional distribution of dendritic cells (DCs)/macrophages in intact liver lobes. Results: Whole-mount imaging at mesoscale revealed that the hepatic classical lobule preferred the oblate ellipsoid morphology in the mouse liver and exhibited hepatic sinusoids with heterogeneous arrangement and intricate loop structure. Liver fibrosis not only induces sinusoidal density increase but also promotes sinusoidal arrangement with increased sinusoidal branch and loop structure. Meanwhile, we found that CD11c+ DCs followed a lognormal distribution in the hepatic lobules, skewing toward lobular boundary in steady state. CCl4-induced chronic liver injury promoted CD11c+ DC rearrangement at the lobular border before the formation of liver fibrosis. Furthermore, through whole-mount imaging of tumor-immune cell-vascular crosstalk in intact lobes based on liver-CUBIC, we characterized an accumulation of CX3CR1+CCR2+F4/80+ macrophages at metastatic foci in early colorectal liver metastases. Importantly, colorectal cells secrete CCL2 to mobilize CX3CR1+CCR2+F4/80+ macrophages to accumulate at liver micrometastases, and the interruption of CCL2-induced macrophage accumulation inhibits early colonization of metastasis in the liver. Conclusions: Our investigation of the sinusoidal network and DC/macrophage arrangements through the liver-CUBIC approach and whole-mount imaging provide a powerful platform for understanding hepatic circulatory properties and immune surveillance in the liver.

A Mutual-Coupling-Suppressed Dual-Band Dual-Polarized Base Station Antenna Using Multiple Folded-Dipole Antenna

Abstract: An interleaved shared-aperture dual-band dual-polarized base station array antenna is proposed in this article. The lower-band (LB) element is realized by using a multiple folded-dipole antenna (MFDA) and four parasitic loops. To interpret the working principle of the MFDA, a double folded-dipole antenna (DFDA) is firstly analyzed by using the transmission line (TL) model. Then, by combining two bended DFDAs and introducing four parasitic loops, a low cross-band scattering LB element with a high out-of-band rejection level of 16 dB is obtained to cover 2.3–2.7 GHz. The higher-band (HB) element with a wide impedance bandwidth of 42.5% (3.0–4.6 GHz), a high roll-off rate (RoR) of 249.2 dB/GHz, and a high out-of-band rejection level of 17 dB is obtained by introducing a meander line loop (MLL), a rectangular loop (RL), and V-shaped strips (VSS) near the dipole arms. By combining the proposed low-scattering low-pass LB element and the high- RoR high-pass HB element, a novel interleaved shared-aperture dual-band dual-polarized array antenna with a small frequency ratio of 1.46 and a high cross-band isolation level of 25 dB is realized. Due to the low-scattering characteristic and filtering response of the LB element, the radiation patterns of the wideband HB sub-arrays are almost unaffected.

Adaptive optical microscopy via virtual-imaging-assisted wavefront sensing for high-resolution tissue imaging

Abstract: Abstract Adaptive optics (AO) is a powerful tool for optical microscopy to counteract the effects of optical aberrations and improve the imaging performance in biological tissues. The diversity of sample characteristics entails the use of different AO schemes to measure the underlying aberrations. Here, we present an indirect wavefront sensing method leveraging a virtual imaging scheme and a structural-similarity-based shift measurement algorithm to enable aberration measurement using intrinsic structures even with temporally varying signals. We achieved high-resolution two-photon imaging in a variety of biological samples, including fixed biological tissues and living animals, after aberration correction. We present AO-incorporated subtractive imaging to show that our method can be readily integrated with resolution enhancement techniques to obtain higher resolution in biological tissues. The robustness of our method to signal variation is demonstrated by both simulations and aberration measurement on neurons exhibiting spontaneous activity in a living larval zebrafish.

Dissection of the long-range projections of specific neurons at the synaptic level in the whole mouse brain

Abstract: Significance It is a huge challenge to dissect the projection patterns of specific neurons with synaptic information in the whole brain. Here, we establish a pipeline for studying long-range projection patterns at the synaptic level and acquired whole-brain information of parvalbumin neurons in the basal forebrain. The synaptic terminals of parvalbumin neurons are widely distributed in the limbic system, including the hippocampus, thalamus, and mammillary body. In the mouse model of Alzheimer’s disease, we found that synaptic degeneration of parvalbumin neurons occurred in memory-related regions, which are inconsistent with amyloid-β plaque distribution. These works provided anatomical information for understanding how basal forebrain regulates downstream regions and the tools to investigate synaptic connections of specific neurons in the whole brain under physiological and pathological conditions.

Adaptive optical microscopy via virtual-imaging-assisted wavefront sensing for high-resolution tissue imaging

Abstract: Abstract Adaptive optics (AO) is a powerful tool for optical microscopy to counteract the effects of optical aberrations and improve the imaging performance in biological tissues. The diversity of sample characteristics entails the use of different AO schemes to measure the underlying aberrations. Here, we present an indirect wavefront sensing method leveraging a virtual imaging scheme and a structural-similarity-based shift measurement algorithm to enable aberration measurement using intrinsic structures even with temporally varying signals. We achieved high-resolution two-photon imaging in a variety of biological samples, including fixed biological tissues and living animals, after aberration correction. We present AO-incorporated subtractive imaging to show that our method can be readily integrated with resolution enhancement techniques to obtain higher resolution in biological tissues. The robustness of our method to signal variation is demonstrated by both simulations and aberration measurement on neurons exhibiting spontaneous activity in a living larval zebrafish.

Mesoporous silica nanoparticle-encapsulated Bifidobacterium attenuates brain Aβ burden and improves olfactory dysfunction of APP/PS1 mice by nasal delivery

Abstract: Dysbiosis or imbalance of gut microbiota in Alzheimer's disease (AD) affects the production of short-chain fatty acids (SCFAs), whereas exogenous SCFAs supplementation exacerbates brain Aβ burden in APP/PS1 mice. Bifidobacterium is the main producer of SCFAs in the gut flora, but oral administration of Bifidobacterium is ineffective due to strong acids and bile salts in the gastrointestinal tract. Therefore, regulating the levels of SCFAs in the gut is of great significance for AD treatment.We investigated the feasibility of intranasal delivery of MSNs-Bifidobacterium (MSNs-Bi) to the gut and their effect on behavior and brain pathology in APP/PS1 mice.Mesoporous silica nanospheres (MSNs) were efficiently immobilized on the surface of Bifidobacterium. After intranasal administration, fluorescence imaging of MSNs-Bi in the abdominal cavity and gastrointestinal tract revealed that intranasally delivered MSNs-Bi could be transported through the brain to the peripheral intestine. Intranasal administration of MSNs-Bi not only inhibited intestinal inflammation and reduced brain Aβ burden but also improved olfactory sensitivity in APP/PS1 mice.These findings suggested that restoring the balance of the gut microbiome contributes to ameliorating cognitive impairment in AD, and that intranasal administration of MSNs-Bi may be an effective therapeutic strategy for the prevention of AD and intestinal disease.

Three-dimensional visualization of heart-wide myocardial architecture and vascular network simultaneously at single-cell resolution

Abstract: Obtaining various structures of the entire mature heart at single-cell resolution is highly desired in cardiac studies; however, effective methodologies are still lacking. Here, we propose a pipeline for labeling and imaging myocardial and vascular structures. In this pipeline, the myocardium is counterstained using fluorescent dyes and the cardiovasculature is labeled using transgenic markers. High-definition dual-color fluorescence micro-optical sectioning tomography is used to perform heart-wide tissue imaging, enabling the acquisition of whole-heart data at a voxel resolution of 0.32 × 0.32 × 1 μm3. Obtained structural data demonstrated the superiority of the pipeline. In particular, the three-dimensional morphology and spatial arrangement of reconstructed cardiomyocytes were revealed, and high-resolution vascular data helped determine differences in the features of endothelial cells and complex coiled capillaries. Our pipeline can be used in cardiac studies for examining the structures of the entire heart at the single-cell level.

Wideband Differentially-Fed Slot Antenna and Array With Circularly Polarized Radiation for Millimeter-Wave Applications

Abstract: A wideband differentially-fed slot antenna is presented for millimeter-wave (mmWave) applications. A novel method of using stepped corner-shaped slot is first utilized to establish the wideband circularly polarized (CP) radiation. In the configuration of corner-shaped slot, two wide open slots at the ends are utilized for effective orthogonal radiation, while the narrow slot at the center is utilized for power transmission and quadrature phase delay. An equivalent circuit is given to illustrate the inner working principle for CP radiation. In addition, square cuts are etched on the four corners of the radiating patches to further increase the axial ratio (AR) and impedance bandwidth. Based on this design concept, the antenna element was first designed and fabricated for performance verification. Then, a 1×4 linear array with beam scanning performance and a 4×4 planar array with high gain and stable radiation were designed and fabricated. Both the simulated and measured results show that the 1×4 linear array and 4×4 planar array can have wide overlapped impedance and AR bandwidths of 30.6% and 33.6% with thickness of 0.16λ0. The advantages of compact size and wide bandwidth make the presented antenna a good candidate for mmWave applications.

Low-Cost Wide-Angle Beam-Scanning Transmitarray Antennas Using Lens-Loaded Patch Elements: A Proof-of-Concept Study

Abstract: In this letter, a novel design concept to achieve a low-cost wide-angle beam-scanning transmitarray (TA) using lens-loaded patch (LLP) elements is presented. In this design, each TA element consists of a beam switchable transmitting element, a group of receiving elements, and a signal via. The received powers of a 2×2 subarray are first combined and then transmitted to the corresponding LLP element, where the phases of the transmitted signals are manipulated for beamforming. Compared with the conventional designs, the phase shifters (PSs) can be multiplexed and the number of PSs is effectively eliminated by 75%, which significantly decreases the hardware cost. By employing multiple patches as the feed of the lenses, reconfigurable radiation patterns are obtained, and by properly choosing the transmitting patches, the developed TA achieves a wide-angle two-dimensional beam scanning within ±60° with stable gains. To validate the design concept, passive prototypes with a center frequency of 12.5 GHz are simulated, fabricated, and tested. The measured results show that the aperture efficiency is 30.1%, 19.4%, and 14.1% for beams steered to 0°, 30°, and 60° at 12.5 GHz, respectively. The beam coverage of ±60° with a less than 3.5 dB scanning loss in the H-plane is obtained.

Low-Profile Wideband Circularly Polarized Array Antennas Using Integrated Half-Power Quadrature Power Divider

Abstract: Low-profile wideband circularly polarized (CP) array antennas using integrated half-power quadrature power divider (HP-QPD) are presented. A novel HP-QPD is first proposed as an important design concept for CP radiation. Detailed analyses show that the proposed HP-QPD can have half-power CP radiation and the remaining half-power transmission with equal-magnitude but quadrature phase difference. Therefore, it can be utilized for designing a 1 $\times $ 2 CP array antenna. Compared to the traditionally designed single-feed or dual-feed CP counterparts, this 1 $\times $ 2 array antenna can have more than four times wider axial ratio (AR) bandwidth with three CP modes and a simple feed network. This 1 $\times $ 2 array antenna was then designed, fabricated, and measured for performance verification. Both measured and simulated results show that this array antenna can have a wide overlapped impedance and AR bandwidth of 7.9% with a thin dielectric thickness of $0.031\lambda _{0}$ . The 1 $\times $ 2 array antenna was further extended to a 1 $\times $ 8 linear array antenna for high gain CP radiation. Measured results show that unidirectional and symmetrical radiation patterns are achieved with high peak realized gain of 15.4 dBic, low profile, and simple feed network.

Observing Single Cells in Whole Organs with Optical Imaging

Abstract: Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals. Then, we describe optical imaging to achieve three-dimensional spatial discrimination for biological tissues. Due to the milky appearance of tissues, the spatial information blurred deep in the whole organ. Fortunately, strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.

Dual-targeted magnetic mesoporous silica nanoparticles reduce brain amyloid-β burden via depolymerization and intestinal metabolism

Abstract: Rationale: Active removal of excess peripheral amyloid-β (Aβ) can potentially treat Alzheimer's disease (AD). However, the peripheral clearance of Aβ using an anti-Aβ monoclonal antibody (mAb) cannot remove PET-detectable Aβ within the brain. This may be due to the inability of mAb to cross the blood-brain barrier (BBB) to degrade insoluble brain Aβ plaques and block liver dysfunction. Methods: We developed a dual-targeted magnetic mesoporous silica nanoparticle (HA-MMSN-1F12) through surface-coupled Aβ42-targeting antibody 1F12 and CD44-targeting ligand hyaluronic acid (HA). Results: HA-MMSN-1F12 had a high binding affinity toward Aβ42 oligomers (Kd = 1.27 ± 0.34 nM) and revealed robust degradation of Aβ42 aggregates. After intravenous administration of HA-MMSN-1F12 into ten-month-old APP/PS1 mice for three weeks (4 mg/kg/week), HA-MMSN-1F12 could cross the BBB and depolymerize brain Aβ plaques into soluble Aβ species. In addition, it also avoided hepatic uptake and excreted captured Aβ species through intestinal metabolism, thereby reducing brain Aβ load and neuroinflammation and improving memory deficits of APP/PS1 mice. Furthermore, the biochemical analysis showed that HA-MMSN-1F12 did not detect any toxic side effects on the liver and kidney. Thus, the efficacy of HA-MMSN-1F12 is associated with the targeted degradation of insoluble brain Aβ plaques, avoidance of non-specific hepatic uptake, and excretion of peripheral Aβ through intestinal metabolism. Conclusions: The study provides a new avenue for treating brain diseases by excreting disease-causing biohazards using intestinal metabolism.

Robust Multi-Beam Multiplexing Design Based on a Hybrid Beamforming Structure With Nearly Equal Magnitude Analogue Coefficients

Abstract: One novel design considering two practical application constraints based on the interleaved subarray architecture is proposed to achieve multi-beam multiplexing for arbitrary directions to serve corresponding users. One is an equal magnitude constraint imposed on the analogue coefficients so that beamforming can be achieved by pure phase shifters after the normalisation of magnitudes; the other one is a robust constraint against steering vector errors. Designed examples are provided to verify the effectiveness of the proposed method.

Mapping the Function of Whole‐Brain Projection at the Single Neuron Level

Abstract: Axonal projection conveys neural information. The divergent and diverse projections of individual neurons imply the complexity of information flow. It is necessary to investigate the relationship between the projection and functional information at the single neuron level for understanding the rules of neural circuit assembly, but a gap remains due to a lack of methods to map the function to whole‐brain projection. Here an approach is developed to bridge two‐photon calcium imaging in vivo with high‐resolution whole‐brain imaging based on sparse labeling with the genetically encoded calcium indicator GCaMP6. Reliable whole‐brain projections are captured by the high‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST). A cross‐modality cell matching is performed and the functional annotation of whole‐brain projection at the single‐neuron level (FAWPS) is obtained. Applying it to the layer 2/3 (L2/3) neurons in mouse visual cortex, the relationship is investigated between functional preferences and axonal projection features. The functional preference of projection motifs and the correlation between axonal length in MOs and neuronal orientation selectivity, suggest that projection motif‐defined neurons form a functionally specific information flow, and the projection strength in specific targets relates to the information clarity. This pipeline provides a new way to understand the principle of neuronal information transmission.

Femtosecond laser writing of waveguides in zinc oxide crystals: fabrication and mode modulation.

Abstract: We report for the first time on optical waveguides in zinc oxide (ZnO) crystals fabricated by femtosecond laser direct writing. The confocal Raman microscopy under 488 nm laser excitation is used to investigate the micro-modifications of the laser irradiation, and guiding properties are studied via the end-face coupling at 632.8 nm. The mode modulation has been achieved by the adjustment of laser writing parameters. A minimum propagation loss of ∼6 dB/cm is obtained for the double-line waveguide structures. A Y-branch waveguide beam splitter is also fabricated, reaching a splitting ratio of nearly 1:1. The original optical properties in the guiding region have been well preserved, according to the confocal Raman investigation, which suggests potential applications of the ZnO waveguides for integrated photonics and nonlinear optics.

High-fidelity mesoscopic fluorescence molecular tomography based on SSB-Net.

Abstract: The imaging fidelity of mesoscopic fluorescence molecular tomography (MFMT) in reflective geometry suffers from spatial nonuniformity of measurement sensitivity and ill-posed reconstruction. In this study, we present a spatially adaptive split Bregman network (SSB-Net) to simultaneously overcome the spatial nonuniformity of measurement sensitivity and promote reconstruction sparsity. The SSB-Net is derived by unfolding the split Bregman algorithm. In each layer of the SSB-Net, residual block and 3D convolution neural networks (3D-CNNs) can adaptively learn spatially nonuniform error compensation, the spatially dependent proximal operator, and sparsity transformation. Simulations and experiments show that the proposed SSB-Net enables high-fidelity MFMT reconstruction of multifluorophores at different positions within a depth of a few millimeters. Our method paves the way for a practical reflection-mode diffuse optical imaging technique.