Reimar Johne

TL;DR: The NAT method provides a sensitive possibility for HEV testing in connection with raising concerns regarding blood safety, and is compared with different nucleic acid amplification technique (NAT) methods for the detection of HEV in blood components.

TL;DR: Four Escherichia coli-expressed segments of the capsid protein of a German wild boar-derived HEV genotype 3 strain were tested for their diagnostic value in an indirect immunoglobulin G (IgG) ELISA and an indirect ELISA for detection of anti-HEV IgG antibodies in human sera was established and validated.

TL;DR: The opinion reviews current methods for the detection, identification, characterisation and tracing of HEV in food‐producing animals and foods, reviews literature on HEV reservoirs and food‐borne pathways, examines information on the epidemiology ofHEV and its occurrence and persistence in foods, and investigates possible control measures along the food chain.

TL;DR: The VP4-, VP6-, VP7- and NSP5-encoding sequences of eight group A rotaviruses from chickens and turkeys determined here indicate a low degree of sequence similarity with mammalian rotviruses, which might indicate interspecies transmission and reassortment among avian group ARotavirus under field conditions.

TL;DR: Analysis of frozen strawberries for the presence of norovirus shows that heterogeneously distributed noroviruses in frozen strawberries can be detected by applying an optimized combination of sampling procedures, virus extraction methods, and real-time RT-PCR protocols.