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Brian P. English
Researcher at Howard Hughes Medical Institute
Publications - 38
Citations - 7252
Brian P. English is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: Translation (biology) & Chromatin. The author has an hindex of 24, co-authored 36 publications receiving 5901 citations. Previous affiliations of Brian P. English include Science for Life Laboratory & Albert Einstein College of Medicine.
Papers
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Journal ArticleDOI
Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution
Bi-Chang Chen,Wesley R. Legant,Kai Wang,Lin Shao,Daniel E. Milkie,Michael W. Davidson,Chris Janetopoulos,Xufeng S. Wu,John A. Hammer,Zhe Liu,Brian P. English,Yuko Mimori-Kiyosue,Daniel P. Romero,Alex T. Ritter,Alex T. Ritter,Jennifer Lippincott-Schwartz,Lillian K. Fritz-Laylin,R. Dyche Mullins,Diana M. Mitchell,Joshua N. Bembenek,Anne-Cécile Reymann,Ralph Böhme,Stephan W. Grill,Jennifer T. Wang,Geraldine Seydoux,U. Serdar Tulu,Daniel P. Kiehart,Eric Betzig +27 more
TL;DR: A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
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A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
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Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Brian P. English,Wei Min,Antoine M. van Oijen,Kang Taek Lee,Kang Taek Lee,Guobin Luo,Hongye Sun,Hongye Sun,Binny J. Cherayil,Binny J. Cherayil,Samuel C. Kou,X. Sunney Xie +11 more
TL;DR: It is proved that the Michaelis-Menten equation still holds even for a fluctuating single enzyme, but bears a different microscopic interpretation.
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Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Paul W. Tillberg,Fei Chen,Kiryl D. Piatkevich,Yongxin Zhao,Chih-Chieh Yu,Brian P. English,Linyi Gao,Anthony J Martorell,Ho-Jun Suk,Fumiaki Yoshida,Fumiaki Yoshida,Ellen M DeGennaro,Douglas H. Roossien,Guanyu Gong,Uthpala Seneviratne,Steven R. Tannenbaum,Robert Desimone,Dawen Cai,Edward S. Boyden +18 more
TL;DR: ProExM as mentioned in this paper is a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins.
Journal ArticleDOI
Fluctuating enzymes: lessons from single-molecule studies.
TL;DR: A theoretical model based on the generalized Langevin equation (GLE) treatment of Kramers' barrier crossing problem for chemical reactions accounts naturally for the observation of dynamic disorder and highly dispersed kinetics.