Davide Normanno

TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.

TL;DR: This work quantitatively analyse the search process in human cells of Tet repressors searching for a multi-array locus with single-molecule tracking and single-cell protein–DNA association measurements, finding that TetRs explore the nucleus and reach their target by 3D diffusion interspersed with transient interactions with non-cognate sites, consistent with the facilitated diffusion model.

TL;DR: Observations suggest that XRCC4–XLF complexes form mobile sleeve-like structures around DNA that can reconnect the broken ends very rapidly and hold them together, which will lead to clarification of how NHEJ proteins are involved in generating chromosomal translocations.

TL;DR: A correlation between tracer size, its biochemical nature and its mobility is identified and the transition from Brownian to subdiffusive motion can be captured in a minimal phenomenological model.

TL;DR: This work investigates looping dynamics of Type IIE restriction enzymes NaeI and NarI by tracking the Brownian motion of single tethered DNA molecules by directly following DNA looping kinetics of single protein–DNA complexes to obtain loop stability and loop formation times.