S
Sachini U. Siriwardena
Researcher at Wayne State University
Publications - 8
Citations - 298
Sachini U. Siriwardena is an academic researcher from Wayne State University. The author has contributed to research in topics: Phosphorylation & APOBEC3A. The author has an hindex of 4, co-authored 5 publications receiving 210 citations. Previous affiliations of Sachini U. Siriwardena include Broad Institute.
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Journal ArticleDOI
A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9.
Basudeb Maji,Basudeb Maji,Basudeb Maji,Soumyashree A. Gangopadhyay,Soumyashree A. Gangopadhyay,Soumyashree A. Gangopadhyay,Miseon Lee,Miseon Lee,Mengchao Shi,Mengchao Shi,Mengchao Shi,Peng Wu,Peng Wu,Peng Wu,Robert Heler,Beverly Mok,Beverly Mok,Donghyun Lim,Donghyun Lim,Sachini U. Siriwardena,Bishwajit Paul,Bishwajit Paul,Bishwajit Paul,Vlado Dančík,Amedeo Vetere,Michael F. Mesleh,Luciano A. Marraffini,Luciano A. Marraffini,David R. Liu,David R. Liu,David R. Liu,Paul A. Clemons,Bridget K. Wagner,Amit Choudhary,Amit Choudhary,Amit Choudhary +35 more
TL;DR: A generalizable platform is reported that provided the first synthetic small-molecule inhibitors of Streptococcus pyogenes Cas9 (SpCas9) that weigh <500 Da and are cell permeable, reversible, and stable under physiological conditions.
Journal ArticleDOI
Functions and Malfunctions of Mammalian DNA-Cytosine Deaminases
TL;DR: The AID/APOBEC family enzymes convert cytosines in single-stranded DNA to uracils, causing base substitutions and strand breaks, and they help combat infections through diverse mechanisms.
Journal ArticleDOI
Characterization of the Catalytic Domain of Human APOBEC3B and the Critical Structural Role for a Conserved Methionine.
TL;DR: Results shed light on the structural organization of APOBEC3B catalytic domain, its substrate specificity and its possible role in causing genome-wide mutations.
Journal ArticleDOI
A Tumor-Promoting Phorbol Ester Causes a Large Increase in APOBEC3A Expression and a Moderate Increase in APOBEC3B Expression in a Normal Human Keratinocyte Cell Line without Increasing Genomic Uracils
Sachini U. Siriwardena,Madusha L. W. Perera,Vimukthi Senevirathne,Jessica A. Stewart,Ashok S. Bhagwat +4 more
TL;DR: It is suggested that the proinflammatory PMA is unlikely to promote extensive APOBEC3A/APOBec3B-mediated cytosine deaminations in human keratinocytes.
Journal ArticleDOI
APOBEC3 enzymes mediate efficacy of cisplatin and are epistatic with base excision repair and mismatch repair in platinum response
Kayla L. Conner,Asra N. Shaik,Katie A Marshall,Ashley M. Floyd,Elmira Ekinci,Jacob Lindquist,Akshada Sawant,Wen Lei,Madison B. Adolph,Linda Chelico,Sachini U. Siriwardena,Ashok S. Bhagwat,Seongho Kim,Michele L. Cote,Steve M. Patrick +14 more
TL;DR: APOBEC3 (A3) enzymes are shown to be capable of deaminating the extrahelical cytosines to uracils and sensitizing breast cancer cells to cisplatin, and it is proposed that A3-induced cytosine deamination to urACil at cis Platin ICLs results in repair of uracil by BER, which blocks ICL DNA repair and enhances cis platin efficacy and improves breast cancer outcomes.