S
Shinichiro Chuma
Researcher at Kyoto University
Publications - 55
Citations - 5047
Shinichiro Chuma is an academic researcher from Kyoto University. The author has contributed to research in topics: Piwi-interacting RNA & Germ cell. The author has an hindex of 36, co-authored 54 publications receiving 4490 citations. Previous affiliations of Shinichiro Chuma include Waseda University & National Institute of Genetics.
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Journal ArticleDOI
Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing
Michael Reuter,Philipp Berninger,Shinichiro Chuma,Hardik Shah,Mihoko Hosokawa,Charlotta Funaya,Claude Antony,Ravi Sachidanandam,Ramesh S. Pillai +8 more
TL;DR: It is shown that Miwi is a small RNA-guided RNase (slicer) that requires extensive complementarity for target cleavage in vitro and supports a slicer-dependent silencing mechanism that functions without piRNA amplification.
Journal ArticleDOI
Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members
Vasily V. Vagin,James A. Wohlschlegel,Jun Qu,Zophonías O. Jónsson,Xinhua Huang,Shinichiro Chuma,Angélique Girard,Ravi Sachidanandam,Gregory J. Hannon,Alexei A. Aravin +9 more
TL;DR: Recognition of methylarginine marks by Tudor proteins can drive the localization of Piwi proteins to cytoplasmic foci in an artificial setting, supporting a role for this interaction in Piwi localization to nuage.
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The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
Masanobu Shoji,Takashi Tanaka,Mihoko Hosokawa,Michael Reuter,Alexander Stark,Yuzuru Kato,Gen Kondoh,Katsuya Okawa,Takeshi Chujo,Tsutomu Suzuki,Kenichiro Hata,Sandra L. Martin,Toshiaki Noce,Satomi Kuramochi-Miyagawa,Toru Nakano,Hiroyuki Sasaki,Ramesh S. Pillai,Norio Nakatsuji,Shinichiro Chuma +18 more
TL;DR: It is shown that tudor-domain containing 9 (Tdrd9) is essential for silencing Line-1 retrotransposon in the mouse male germline, and the results identify TDRD9 as a functional partner of MIWI2 and indicate that the tUDor-piwi association is a conserved feature.
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Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile.
Michael Reuter,Shinichiro Chuma,Takashi Tanaka,Thomas Franz,Alexander Stark,Ramesh S. Pillai +5 more
TL;DR: This work isolated Mili-containing complexes from adult mouse testes and identified Tudor domain–containing protein-1 (Tdrd1) as a factor specifically associated with the Mili piRNP throughout spermatogenesis, suggesting that Tdrd 1 ensures the entry of correct transcripts into the normal piRNA pool.
Journal ArticleDOI
MITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline
Toshiaki Watanabe,Shinichiro Chuma,Yasuhiro Yamamoto,Yasuhiro Yamamoto,Satomi Kuramochi-Miyagawa,Yasushi Totoki,Atsushi Toyoda,Yuko Hoki,Yuko Hoki,Asao Fujiyama,Tatsuhiro Shibata,Takashi Sado,Takashi Sado,Toshiaki Noce,Toru Nakano,Norio Nakatsuji,Haifan Lin,Hiroyuki Sasaki,Hiroyuki Sasaki +18 more
TL;DR: In mutant germ cells, mitochondria and the components of the nuage, a perinuclear structure involved in piRNA biogenesis/function, are mislocalized to regions around the centrosome, suggesting that MITOPLD may be involved in microtubule-dependent localization of mitochondria