S
Shouzhuo Yao
Researcher at Hunan University
Publications - 501
Citations - 17343
Shouzhuo Yao is an academic researcher from Hunan University. The author has contributed to research in topics: Cyclic voltammetry & Biosensor. The author has an hindex of 66, co-authored 483 publications receiving 15864 citations. Previous affiliations of Shouzhuo Yao include Hunan Normal University.
Papers
More filters
Journal ArticleDOI
Determination of urea in urine using a conductivity cell with surface acoustic wave resonator-based measurement circuit
TL;DR: The effects of pH, temperature and inhibitor on the response of the enzyme conductivity measurement system were investigated and the system was applied to rapid determination of urea in small urine samples.
Journal ArticleDOI
Phosphorylation-Mediated Assembly of a Semisynthetic Fluorescent Protein for Label-Free Detection of Protein Kinase Activity
Chao Yin,Ming Wang,Chunyang Lei,Zhen Wang,Pei Li,Yong Li,Wang Li,Yan Huang,Zhou Nie,Shouzhuo Yao +9 more
TL;DR: A novel fluorescent biosensor to detect protein kinase activity based on phosphorylation-mediated assembly of semisynthetic green fluorescent protein (GFP) that is facile, sensitive, label-free, and highly versatile, thus showing great potential as a promising platform forprotein kinase detection and inhibitor screening.
Journal ArticleDOI
α-Amylase immobilized on bulk acoustic-wave sensor by UV-curing coating
TL;DR: The experimental results show that the immobilized alpha-amylase entrapped by the UV-curing coating retains adequate enzymatic activity and can be reused more than 50 times under certain experimental conditions.
Journal ArticleDOI
Characterization and electrocatalytic properties of cobalt hexacyanoferrate films immobilized on Au-colloid modified gold electrodes
TL;DR: In this paper, an electroactive cobalt hexacyanoferrate (CoHCF) film was electrodeposited from a solution containing Co 2+ and Fe(CN) 6 3− ions on the bare gold or the Au-colloid modified electrode.
Journal ArticleDOI
Analysis of perfluorooctane sulfonate and perfluorooctanoic acid with a mixed-mode coating-based solid-phase microextraction fiber.
TL;DR: The proposed method was successfully applied in the analysis of PFOS and PFOA in a local river and shows excellent organic solvent stability due to the covalent bonding between the coating and the fiber, and significantly higher extraction efficiency than the commercial fibers.