Stefanie Vehring

TL;DR: Evidence is provided that StarD10 interacts with phosphatidylcholine (PC) andosphatidylethanolamine (PE) by electron spin resonance measurement and preferentially selects lipid species containing a palmitoyl or stearoyl chain on the sn-1 and an unsaturated fatty acyl chain (18:1 or 18:2) on thesn-2 position.

TL;DR: It is demonstrated that shape change of giant unilamellar vesicles (GUVs) can be used as a new tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids.

TL;DR: Results show that the flippase is able to translocate all four PI isomers and that both glycerol isomers of PI flip-flop across the ER membrane at rates similar to that measured for fluorescence-labeled phosphatidylcholine.

TL;DR: In this paper, the authors investigate whether the endoplasmic reticulum (ER) flippase recognizes the glycerophospholipids that it transports, and they show that the flippases is able to translocate all four diastereoisomers of PI across the ER membrane at rates similar to that measured for fluorescence-labeled phosphatidylcholine.

TL;DR: It is found that the protein translocon is not required for transbilayer movement of phospholipids across the ER, and yeast is defined as a promising model system for future attempts to identify the ER phosphorus flippase and to test and purify candidate flippases.