Steffen J. Sahl

TL;DR: In this Review,luorescence nanoscopy uniquely combines minimally invasive optical access to the internal nanoscale structure and dynamics of cells and tissues with molecular detection specificity and the labelling of individual molecules to enable their visualization has emerged as a central challenge.

TL;DR: Super-resolution optical microscopy is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.

TL;DR: This work proposes and demonstrates a framework for pupil-plane modulation for 3D imaging applications requiring precise localization, including single-particle tracking and superresolution microscopy, and generates and experimentally demonstrates two example PSFs.

TL;DR: Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio) physical and biomedical research, particularly with respect to biomedical research.

TL;DR: A proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins.