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Bi-Chang Chen
Researcher at Academia Sinica
Publications - 67
Citations - 5883
Bi-Chang Chen is an academic researcher from Academia Sinica. The author has contributed to research in topics: Light sheet fluorescence microscopy & Microscopy. The author has an hindex of 25, co-authored 66 publications receiving 4724 citations. Previous affiliations of Bi-Chang Chen include Howard Hughes Medical Institute & National Taiwan University.
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Journal ArticleDOI
Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution
Bi-Chang Chen,Wesley R. Legant,Kai Wang,Lin Shao,Daniel E. Milkie,Michael W. Davidson,Chris Janetopoulos,Xufeng S. Wu,John A. Hammer,Zhe Liu,Brian P. English,Yuko Mimori-Kiyosue,Daniel P. Romero,Alex T. Ritter,Alex T. Ritter,Jennifer Lippincott-Schwartz,Lillian K. Fritz-Laylin,R. Dyche Mullins,Diana M. Mitchell,Joshua N. Bembenek,Anne-Cécile Reymann,Ralph Böhme,Stephan W. Grill,Jennifer T. Wang,Geraldine Seydoux,U. Serdar Tulu,Daniel P. Kiehart,Eric Betzig +27 more
TL;DR: A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
Journal ArticleDOI
Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics
Dong Li,Lin Shao,Bi-Chang Chen,Xi Zhang,Xi Zhang,Mingshu Zhang,Brian Moses,Daniel E. Milkie,Jordan R. Beach,John A. Hammer,Mithun Pasham,Tomas Kirchhausen,Michelle A. Baird,Michelle A. Baird,Michael W. Davidson,Pingyong Xu,Eric Betzig +16 more
TL;DR: Two approaches are used to improve the resolution of SIM to allow live cell imaging of dynamic cellular processes, including endocytosis and cytoskeleton remodeling, and it is demonstrated that the resolution for these methods is comparable with or better than other SR approaches yet allowed us to image at far higher speeds, and for far longer durations.
Journal ArticleDOI
Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Jiji Chen,Zhengjian Zhang,Li M. Li,Bi-Chang Chen,Andrey Revyakin,Bassam Hajj,Wesley R. Legant,Maxime Dahan,Maxime Dahan,Timothée Lionnet,Eric Betzig,Robert Tjian,Zhe Liu +12 more
TL;DR: In vivo and in vitro single-molecule imaging, transcription factor (TF) mutagenesis, and ChIP-exo mapping are combined to determine how TFs dynamically search for and assemble on their cognate DNA target sites and find that enhanceosome assembly is hierarchically ordered.
Journal ArticleDOI
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans.
Jennifer T. Wang,Jarrett Smith,Bi-Chang Chen,Helen Schmidt,Dominique Rasoloson,Alexandre Paix,Bramwell G. Lambrus,Deepika Calidas,Eric Betzig,Geraldine Seydoux +9 more
TL;DR: Evidence is presented that a group of intrinsically disordered, serine-rich proteins regulate the dynamics of P granules in C. elegans embryos, and it is concluded that P granule assembly in embryos is regulated by phosphorylation.
Journal ArticleDOI
A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro
Shukry J. Habib,Shukry J. Habib,Bi-Chang Chen,Feng-Chiao Tsai,Konstantinos Anastassiadis,Tobias Meyer,Eric Betzig,Roel Nusse +7 more
TL;DR: It is suggested that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.