Tomomi Tani

TL;DR: It is demonstrated that nucleosomes form compact domains with a peak diameter of ∼160 nm and move coherently in live cells and Notably, the domains during mitosis are observed, suggesting that they act as building blocks of chromosomes and may serve as information units throughout the cell cycle.

TL;DR: It is proposed that this local nucleosome fluctuation is the basis for scanning genome information in living mammalian cells and inhibition of these local dynamics by crosslinking impaired accessibility in the dense chromatin regions.

TL;DR: This work defines a key role of the membrane in directing septin filament formation in live cells and reconstituted dynamic septin polymerization, using purified components and finds that septins grow into filaments and form higher-order structures by diffusing, colliding, and annealing on the plasma membrane.

TL;DR: This work investigates genome-wide chromatin behavior under various transcriptional conditions in living human cells using single-nucleosome imaging and proposes the existence of loose chromatin domain networks for various intra-/interchromosomal contacts via active RNAPII clusters/droplets, which is compatible with models of classical transcription factories or liquid droplet formation of transcription-related factors.

TL;DR: A pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date is reported, allowing prolonged visualization of biological events in an acidic environment.