Vincent A. Pieribone

TL;DR: Dense, focal injections of wheat germ agglutinin conjugated-horseradish peroxidase in the locus coeruleus of rats labeled afferent neurons in unexpectedly few brain regions, redefine the anatomic organization of the loci coerulesus, and have implications for hypotheses concerning the functions of this noradrenergic brain nucleus.

TL;DR: Perturbation of synapsin function resulted in a selective disruption of the reserve pool of vesicles and in addition, led to an inhibition and slowing of the kinetics of neurotransmitter release, indicating a second role for synapsins downstream from vesicle docking.

TL;DR: It is concluded that 5-HT inputs may derive from local5-HT neurons in the pericoerulear area of the nucleus locus coeruleus, and the dorsal raphe does not provide the robust 5- HT innervation found in the LC.

TL;DR: The development of a fluorescent protein voltage sensor, named ArcLight, and derivative probes that exhibit large changes in fluorescence intensity in response to voltage changes are reported, which allows the reliable detection of single action potentials and excitatory potentials in individual neurons and dendrites.

TL;DR: It is shown that clusters of vesicles at synaptic release sites are composed of two pools, a distal pool containing synapsin and a proximal pool devoid of synAPSin and located adjacent to the presynaptic membrane.