Won-Ki Cho

TL;DR: This work used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly and suggests that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.

TL;DR: A live-cell super-resolution approach is developed to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus and suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.

TL;DR: It is determined that the mismatch repair (MMR) initiation protein MutS forms a transient clamp that scans duplex DNA for mismatched nucleotides by 1D diffusion for 1 s (~700 base pairs) while in continuous rotational contact with the DNA.

TL;DR: It is shown that ATP binding alters the MutS diffusion mechanics on DNA, which has a number of implications for the mechanism of MMR.

TL;DR: In this article, the endogenous RNA polymerase II (Pol II) was labeled in mouse embryonic fibroblast (MEF) cells using the CRISPR/Cas9 gene editing system using single-molecule based super-resolution imaging in the living cells.