Ibrahim I Cisse

TL;DR: It is postulated that super-enhancers are phase-separated multimolecular assemblies, also known as biomolecular condensates, which provide a means to compartmentalize and concentrate biochemical reactions within cells.

TL;DR: It is shown experimentally and with a new simulation algorithm that ellipsoids can randomly pack more densely and suggested that the higher density is directly related to the higher number of degrees of freedom per particle and thus the larger number of particle contacts required to mechanically stabilize the packing.

TL;DR: This work used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly and suggests that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.

TL;DR: A quantitative single-cell approach to characterize protein spatiotemporal organization, with single-molecule sensitivity in live eukaryotic cells, suggests that transient crowding of enzymes may aid in rate-limiting steps of gene regulation.

TL;DR: In this paper, the authors investigated whether the phosphorylation of the C-terminal domain of the RNA polymerase II (PolII) C-interaction subunit regulates the incorporation of Pol II into phase-separated condensates that are associated with transcription initiation and splicing.