Y
Yun Huang
Researcher at Texas A&M University
Publications - 137
Citations - 10404
Yun Huang is an academic researcher from Texas A&M University. The author has contributed to research in topics: DNA methylation & Gene. The author has an hindex of 33, co-authored 124 publications receiving 8762 citations. Previous affiliations of Yun Huang include Texas A&M Health Science Center College of Medicine & Brigham and Women's Hospital.
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Journal ArticleDOI
A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication.
Yubin Zhou,Wen-Pin Tzeng,Yiming Ye,Yun Huang,Shunyi Li,Yanyi Chen,Teryl K. Frey,Jenny J. Yang +7 more
TL;DR: It is demonstrated that the CXXC(X)(48)CXC Zn(2+)-binding motif in the RUBV NS protease is critical for maintaining the structural integrity of the protease domain and essential for proteolysis and virus replication.
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Dietary spinach reshapes the gut microbiome in an Apc-mutant genetic background: mechanistic insights from integrated multi-omics.
Ying-Shiuan Chen,Jia Li,Rani Menon,Arul Jayaraman,Kyongbum Lee,Yun Huang,Wan Mohaiza Dashwood,Ke Zhang,Deqiang Sun,Roderick H. Dashwood +9 more
Abstract: Complex interrelationships govern the dynamic interactions between gut microbes, the host, and exogenous drivers of disease outcome. A multi-omics approach to cancer prevention by spinach (SPI) was pursued for the first time in the polyposis in rat colon (Pirc) model. SPI fed for 26 weeks (10% w/w, freeze-dried in the diet) exhibited significant antitumor efficacy and, in the Apc-mutant genetic background, β-catenin remained highly overexpressed in adenomatous polyps. However, in both wild type and Apc-mutant rats, increased gut microbiome diversity after SPI consumption coincided with reversal of taxonomic composition. Metagenomic prediction implicated linoleate and butanoate metabolism, tricarboxylic acid cycle, and pathways in cancer, which was supported by transcriptomic and metabolomic analyses. Thus, tumor suppression by SPI involved marked reshaping of the gut microbiome along with changes in host RNA-miRNA networks. When colon polyps were compared with matched normal-looking tissues via metabolomics, anticancer outcomes were linked to SPI-derived linoleate bioactives with known anti-inflammatory/ proapoptotic mechanisms, as well as N-aceto-2-hydroxybutanoate, consistent with altered butanoate metabolism stemming from increased α-diversity of the gut microbiome. In colon tumors from SPI-fed rats, L-glutamate and N-acetylneuraminate also were reduced, implicating altered mitochondrial energetics and cell surface glycans involved in oncogenic signaling networks and immune evasion. In conclusion, a multi-omics approach to cancer prevention by SPI provided mechanistic support for linoleate and butanoate metabolism, as well as tumor-associated changes in L-glutamate and N-acetylneuraminate. Additional factors, such as the fiber content, also warrant further investigation with a view to delaying colectomy and drug intervention in at-risk patients.
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Designing Caspase-3 Sensors for Imaging of Apoptosis in Living Cells
TL;DR: The mechanism of caspase activation during apoptosis is not fully understood and investigations have been hampered by a lack of real-time sensors with high enzymatic specificity to quantitatively detect caspases activity in live cells or in vivo.
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Sphingolipids are involved in N-methyl-N'-nitro-N-nitrosoguanidine-induced epidermal growth factor receptor clustering.
TL;DR: Data suggested that sphingolipids are involved in MNNG-induced receptor clustering; however, the specific species involved may be different from those involved in EGF-mediated receptors clustering.
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Design of Smart Antibody Mimetics with Photosensitive Switches
TL;DR: A generally applicable method to engineer light-controllable monobodies and nanobodies, designated as moonbody and sunbody, respectively, that enable rapid and reversible antibody-antigen recognition by utilizing light.