Damietta University

About: Damietta University is a education organization based out in Damietta, Egypt. It is known for research contribution in the topics: Thin film & Ligand. The organization has 953 authors who have published 1730 publications receiving 28583 citations. The organization is also known as: DU.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Whale Optimization Algorithm and Moth-Flame Optimization for multilevel thresholding image segmentation

Abstract: Two metaheuristic algorithms (WOA and MFO) are used.These algorithms are applied to multilevel thresholding image segmentation.MFO and WOA are better than compared algorithms.MFO is better than WOA for higher number of thresholds. Determining the optimal thresholding for image segmentation has got more attention in recent years since it has many applications. There are several methods used to find the optimal thresholding values such as Otsu and Kapur based methods. These methods are suitable for bi-level thresholding case and they can be easily extended to the multilevel case, however, the process of determining the optimal thresholds in the case of multilevel thresholding is time-consuming. To avoid this problem, this paper examines the ability of two nature inspired algorithms namely: Whale Optimization Algorithm (WOA) and Moth-Flame Optimization (MFO) to determine the optimal multilevel thresholding for image segmentation. The MFO algorithm is inspired from the natural behavior of moths which have a special navigation style at night since they fly using the moonlight, whereas, the WOA algorithm emulates the natural cooperative behaviors of whales. The candidate solutions in the adapted algorithms were created using the image histogram, and then they were updated based on the characteristics of each algorithm. The solutions are assessed using the Otsus fitness function during the optimization operation. The performance of the proposed algorithms has been evaluated using several of benchmark images and has been compared with five different swarm algorithms. The results have been analyzed based on the best fitness values, PSNR, and SSIM measures, as well as time complexity and the ANOVA test. The experimental results showed that the proposed methods outperformed the other swarm algorithms; in addition, the MFO showed better results than WOA, as well as provided a good balance between exploration and exploitation in all images at small and high threshold numbers.