Showing papers by "Lund University published in 1969"
TL;DR: The Frozen Shoulder: Clinical and Radiographical Observations the Effect of Manipulation under General Anesthesia Structure and Glycosaminoglycan Content of the Joint Capsule Local Bone Metabolism.
Abstract: (1969). The Frozen Shoulder: Clinical and Radiographical Observations the Effect of Manipulation Under General Anesthesia Structure and Glycosaminoglycan Content of the Joint Capsule Local Bone Metabolism. Acta Orthopaedica Scandinavica: Vol. 40, No. sup119, pp. 1-59.
285 citations
TL;DR: The crude protein fraction of human duodenal contents catalyzed the formation of ceramide from sphingomyelin most efficiently at slightly alkaline pH in the presence of conjugated bile salts, indicating that the enzymes are of intestinal origin.
202 citations
TL;DR: In the groups the two metabolites were positively correlated, in agreement with earlier investigations showing disturbances of monoamine metabolism in the brain of patients suffering from these syndromes.
Abstract: — Four groups, one of healthy volunteers, one of senile dementia, one of presenile dementia, and one of patients with parkinsonism were compared according to the values of HVA and 5-HIAA in CSF. The levels of HVA and 5-HIAA were highest in the group of healthy volunteers and decreased in the following order: senile dementia, presenile dementia, and parkinsonism. In the groups the two metabolites were positively correlated. These results are in agreement with earlier investigations showing disturbances of monoamine metabolism in the brain of patients suffering from these syndromes.
170 citations
TL;DR: In this article, the tris(hydroxymethyl) aminomethane, also known as “tham”, was used as a test substance for solution calorimetry.
151 citations
TL;DR: The mucosal reactions to high transmural pressures support the theory of vascular hypotonicity in the rhinitic nose and common colds should not be “cured” with bed rest; at night, rhinitics patients should take up a body position of more than 204dG above the horizontal plane.
Abstract: Comparative quantitative measurements of the nasal airway resistance (Rn) in different positions were performed in normal individuals, in patients with allergic rhinitis, and in patients with common colds during and after infection. In normal persons and in patients recovered from common colds Rn increased only very little in positions of 20° or less above the horizontal plane. In rhinitic patients the same positions were followed by a considerable increase of Rn caused by swelling of the. nasal mucosa due to hydrostatic blood pressure increase. This was proved by the effect of neck vein compression causing about the same increase of Rn as the horizontal position. The mucosal reactions to high transmural pressures support the theory of vascular hypotonicity in the rhinitic nose. In view of the biological and clinical consequences of the postural effects on the nasal mucosa, common colds should not be “cured” with bed rest; at night, rhinitic patients should take up a body position of more than 204dG above...
138 citations
TL;DR: The present experiments were undertaken to explore what information could be derived from fecal analysis after a single feeding of meals containing labeled cholesterol, and it was found that the plant sterol &sitosterol is absorbed from the human intestine.
124 citations
123 citations
TL;DR: Microspectrofluometry revealed dopamine in the perch and trout retinal neurons, and it cannot be excluded that some horizontal cells contain a catecholamine.
Abstract: The adrenergic retinal neurons of perch and trout were studied with the fluorescence microscopical method of Falck and Hillarp. Pilot studies were also performed on pike, plaice, cod, eel, goldfish, cunner, black moor, cichlid and carp. Only minor differences were noted between the species.
123 citations
TL;DR: The “neutral” and “acid” β-galactosidases of rat small intestine can be measured separately when present in mixture, and the accuracy of these methods has been tested by measurement of the activity of mixtures containing known amounts of both enzymes.
121 citations
TL;DR: Dextransucrase from Streptococcus sanguis was isolated from the supernatant fluid of a glucose-containing broth culture and purification of this constitutive enzyme was achieved in two steps by adsorption on hydroxylapatite followed by iso-electric focusing and fractionation.
117 citations
TL;DR: A patient with mannosidosis, a storage disorder which has some clinical resemblance to Hurler's syndrome, is described, and a deficiency of the enzyme α-mannosidase in the liver is disclosed, leading to imparied glycoprotein catabolism with storage of oligosaccharide containing mannose and glucosamines.
TL;DR: Cholesterol and lecithin absorption in man was studied by intestinal intubation using β-3H-sitosterol lipid-soluble marker and Phospholipids partitioned in a glyceride bile salt mixture in favour of the bile Salt micellar phase.
Abstract: Cholesterol and lecithin absorption in man was studied by intestinal intubation. β-3H-sitosterol lipid-soluble marker. Cholesterol absorption was limited and progressed over the length of the small intestine; most extensive in the upper part. Lecithin was hydrolyzed to L-α-lysolecithin and free fatty acids, which were absorbed in parallel in the upper small intestine. 3.5–6.5 g lecithin and 0.2–0.4 g cholesterol were delivered via the bile during passage of a test meal. Phospholipids partitioned in a glyceride bile salt mixture in favour of the bile salt micellar phase.
TL;DR: The distribution of α1-antitrypsin among the Pi zones is different from the normal in the allele products of Pi1 and Pi2, and the concentration of this protein is lower thannormal in the serum from subjects of phenotype SS, MS, ZZ and MZ.
Abstract: Each Pi allele, determining a genetic variant of α1-antitrypsin, results in eight protein zones when human serum is subjected to electrophoresis in starch gels, pH 4.9. Antigen-antibody crossed electrophoresis was adapted to the immunological quantitation of proteins separated by starch gel electrophoresis. The distribution of α1-antitrypsin among the Pi zones is different from the normal in the allele products of Pi1 and Pi2. The concentration of this protein is lower than normal in the serum from subjects of phenotype SS, MS, ZZ and MZ.
TL;DR: The accumulated evidence on the ecology of Streptococcus sanguis and StrePTococcus mutans indicate that these streptococci can only be established in the mouth if solid surfaces such as on teeth and dentures are present.
TL;DR: Gray matter of the brain from a patient with a new storage disease was extracted with water and it is suggested that the new disease be termed mannosidosis, referring to the mannoserich oligosaccharide material which is stored.
TL;DR: The analyses of the small intestine and intestinal contents 2–3 h after feeding the substrates indicated that the hydrolysis of dietary cerebroside occurs in the mucosal cells after absorption, and an active biosynthesis of cerebro side, in which dietary sphingosin bases take part, occurs inThe mucosalcells after absorption.
TL;DR: Focal potentials in the main and external cuneate nuclei and in the rostral part of the cervical spinal cord evoked by volleys in forelimb muscle and cutaneous nerves have been studied and group I activated cells organized in a somatotopic manner are recorded.
TL;DR: Insight is obtained into this paradox by analyzing a simple Gauss-Markov process and it is shown that there is an optimal choice of h and that the variance increases rapidly when h increases from the optimal value.
TL;DR: The proportions of different triacylglycerol fractions were predicted from the assumption that each fatty acid in any position is combined with fatty acids in proportion to their relative amounts in the other positions, and the observed distribution agreed well with the predicted one-.
Abstract: 1
Different fractions of rat liver triacylglycerols and X-1,2-diacylglycerols were prepared by thin-layer chromatography on silver nitrate-containing silica gel.
2
The positional distribution of fatty acids in these fractions and unfractionated triacylglycerols and diacylglycerols was analyzed by a scaled-down version of Brockerhoff's method.
3
Both in tri- and diacylglycerols position 1 contains mainly saturated fatty acids, while most fatty acids in position 2 are unsaturated. Position 3 in triacylglycerols contains mainly unsaturated fatty acids and has a large proportion of highly unsaturated fatty acids.
4
The relative abundance of different 1,2-diacyl-sn-glycerol units in diacylglycerols and triacylglycerols was similar except for highly unsaturated units. This suggests a random utilization of 1,2-diacyl-sn-glycerols with 1–3 double bonds for triacylglycerol biosynthesis. The highly unsaturated diacylglycerols probably originate from phospholipids.
5
From the positional distribution of fatty acids in total triacylglycerols the proportions of different triacylglycerol fractions were predicted from the assumption that each fatty acid in any position is combined with fatty acids in proportion to their relative amounts in the other positions. The observed distribution agreed well with the predicted one-.
TL;DR: It is concluded that the development and chronic state of diabetes in mice following streptozotocin and alloxan, respectively, are not identical.
TL;DR: Various regions of the human stomach were analyzed histochemically for the cellular localization of monoamines and histamine, and radiometrically forThe detection of histidine decarboxylase activity.
Abstract: Various regions of the human stomach were analyzed histochemically for the cellular localization of monoamines and histamine, and radiometrically for the detection of histidine decarboxylase activity.
1.
Gastric histamine is located exclusively in mast cells evenly distributed throughout the entire thickness of the mucosa and submucosa.
2.
No detectable histidine decarboxylase activity occurs in any of the regions.
3.
5-Hydroxytryptamine is located in enterochromaffin cells which increase in number from the corpus towards the antrum. There is evidence that some of these cells receive an adrenergic innervation.
4.
After incubation of tissue specimens with l-DOPA another system of cells becomes visible in the microscope owing to their green formaldehyde-induced fluorescence, probably due to dopamine formed intracellularly. Their morphology resembles that of the enterochromaffin cells and they have hence been termed “enterochromaffin-like”. The regional distribution of these cells is similar to that of the enterochromaffin cells.
5.
Adrenergic nerve terminals from the submucosa extend into the mucosa to run in the lamina propria close to the epithelium.
TL;DR: Three patients with light-blue tattoos developed tattoo granulomas and, simultaneously, uveitis and two of the patients showed delayed allergic reaction to cobalt (the substance probably used in the light- blue tattoos).
TL;DR: The ventilatory capacity of the Eustachian tube was studied quantitatively in subjects with normal tubal function in different postures and during compression of the neck veins.
Abstract: It has been well known for a long time that the patency of the Eusta-chian tube is affected by body position, particularly in patients with patulous tubes. The ventilatory capacity of the Eustachian tube was studied quantitatively in subjects with normal tubal function in different postures and during compression of the neck veins. In the horizontal position or during compression of the neck veins with 35 cm H2O the volumes passing through the tube during deglutition were reduced to 1/3in a body elevation of 20° or 30° the tubal function quantitatively decreased to 2/3. When the subject was lying on one side the tube of the downturned ear seemed most affected. In subjects who caught colds the consequence of horizontal position was very marked.Clinicians are familiar with the fact that the symptoms of acute otitis media often start at night when the patient has been in bed for some hours. During upper respiratory infections or after intratympanic surgery, when tubal dysfunction may be expected, patients sh...
TL;DR: The marked differences even between animals as closely related as some platyrhine monkeys call for caution when comparing the detailed function of the retina in different animals.
Abstract: The retina of Aotes monkeys, Cebus monkeys, squirrel monkeys, and marmosets were investigated. Adrenergic perikarya were found in the innermost cell rows of the inner nuclear layer of all the investigated species. In addition, the Cebus monkey was found to have a special type of adrenergic neurons in the inner nuclear layer. This cell type was called the adrenergic pleomorph cell. Its processes ramify in the inner nuclear and inner plexiform layers. Adrenergic terminals occur in three more or less well developed sublayers of the inner plexiform layer, the layers being best developed in the Cebus monkey. Adrenergic terminals were also found around the cells of the inner nuclear layer and at the horizontal cells, where a scant sublayer is formed. More than one adrenergic sublayer of the inner plexiform layer has not been observed in primates previously, nor have the adrenergic terminals in the inner nuclear layer been observed previously in any species. The adrenergic pleomorph cells of the Cebus monkey also seem to be unique. The marked differences even between animals as closely related as some platyrhine monkeys call for caution when comparing the detailed function of the retina in different animals.
TL;DR: It is concluded that sodium taurodesoxycholate does not change the molecular size or configuration of triolein lipase sufficiently for this to be detected by gel filtration, but does promote the elution of a lipase activator together with the enzyme, resulting in an improved yield of lipase after chromatography.
TL;DR: A very high correlation was found between volume and flow changes in anaesthetized cats with controlled respiration, indicating that variations of regional cerebral blood volume are accompanied by proportional blood flow changes.
Abstract: Blood flow and blood volume were measured simultaneously in the same cerebral region in anaesthetized cats with controlled respiration. The measurements were made with the same scintillation-detector, using the freely diffusible indicator 133 Xenon for flow determinations and the intra-vascular indicator RISA (131I) for volume recordings. A very high correlation was found between volume and flow changes (r = .96, p < 0.001). This finding indicates that variations of regional cerebral blood volume are accompanied by proportional blood flow changes.
TL;DR: An increased reduction in the NADH/NAD+ system of the brain during pronounced hyperventilation is strongly suggested.
Abstract: In order to study the effect of pronounced hyperventilation upon the redox state of brain tissue rats were hyperventilated with air and measurements were made of the cortical level of NADH, using the microfluorometric method of Chance. Control measurements were made of the tissue concentrations of lactate, pyruvate, ATP, ADP, AMP, and phosphocreatine during and after the hyperventilation. It was found that when the rats were hyperventilated to arterial pCO2 levels below about 20 mm Hg, the NADH fluorescence invariably increased. Although part of this increase may represent a hemodynamic artefact due to a decrease in the tissue blood volume, a large part of it should represent a true reduction in the NADH/NAD+ system. With hyperventilations of this degree there was a proportionally larger increase in lactate than in pyruvate and thus an increased lactate/pyruvate ratio, but there were no changes in phosphocreatine, or in adenosine phosphates, except for a small but significant increase in the AMP concentration. The NADH increase during hyperventilation was slightly decreased when pure oxygen was administered and completely normalized when 3–5 % CO2 was given. The experiments thus strongly suggest an increased reduction in the NADH/NAD+ system of the brain during pronounced hyperventilation.
TL;DR: Durch fluoreszenzmikroskopische Untersuchung and nachfolgende Silberfärbung an ein and demselben Schnitt konnte gezeigt werden, dass das System argyrophiler, nicht argentaffiner Zellen in der Magenschleimhaut der Ratte mit demjenigen histaminspeichernder Epithelzellen identisch ist.
Abstract: Durch fluoreszenzmikroskopische Untersuchung und nachfolgende Silberfarbung an ein und demselben Schnitt konnte gezeigt werden, dass das System argyrophiler, nicht argentaffiner Zellen in der Magenschleimhaut der Ratte mit demjenigen histaminspeichernder Epithelzellen identisch ist Die histaminspeichernden Zellen, die argyrophil (aber nicht argentaffin) sind, bilden ein Zellsystem, das von dem der enterochromaffinen, serotoninhaltigen Zellen getrennt ist Diese enterochromaffinen Epithelzellen sind im Gegensatz zu den histaminhaltigen Zellen sowohl argyrophil als auch argentaffin