Montreal Children's Hospital

About: Montreal Children's Hospital is a healthcare organization based out in Montreal, Quebec, Canada. It is known for research contribution in the topics: Population & Poison control. The organization has 3842 authors who have published 4816 publications receiving 200198 citations.

Chronic physical illness in childhood: psychological and social effects in adolescence and adult life.

Abstract: A national birth cohort followed for 36 years was used to compare the life chances of individuals with chronic physical illness in childhood with those of controls. The majority of those who had been chronically physically ill in childhood were found to differ very little in social and psychological circumstances by 36 years of age, but earlier in adult life there had been signs of difficulties. However, there was a disturbing tendency for those from lower social-group families to be in significantly worse social and psychological circumstances, and by 36 years they showed signs of relatively poor life chances and of basic social support, including the death of both parents.

Evaluation of a quantitative DNA methylation analysis technique using methylation-sensitive/dependent restriction enzymes and real-time PCR

Abstract: DNA methylation in mammals has been shown to play many important roles in diverse biological phenomena Several methods have been developed for the measurement of region-specific levels of DNA methylation We sought a technique that could be used to quantitatively evaluate multiple independent loci in several tissues in a quick and cost-effective manner Recently, a few quantitative techniques have been developed by employing the use of real-time PCR, though they require the additional step of sodium bisulfite conversion Here we evaluate a technique that involves the digestion of non-sodium bisulfite-treated genomic DNA using methylation-sensitive and methylation-dependent restriction enzymes followed by real-time PCR The utility of this method is tested by analyzing seventeen genomic regions of known tissue-specific levels of DNA methylation including three imprinted genes We find that this approach generates rapid, reproducible and accurate results (range = +/-5%) without the additional time required for bisulfite conversion This approach is also adaptable for use with smaller amounts of starting material We propose this method as a rapid, quantitative method for the analysis of DNA methylation at single sites or within small regions of DNA