Showing papers by "Sapienza University of Rome published in 1971"
TL;DR: The polypeptide components of the very low density, low density and high density apolipoproteins were fractionated and partially characterized and had distinct characteristics in terms of amino acid composition, disc electrophoretic mobility and immunochemical reactivities.
78 citations
TL;DR: In this paper, the relative efficiency versus energy of a type 4028 Bendix Channeltron has been measured for electron energies in the range 10-1000 eV, using a theoretical curve derived from Lye and Dekker's theory of the secondary emission.
70 citations
TL;DR: A new role for rhodanese is outlined, namely the possibility that the enzyme may contribute to the formation of ‘labile sulfur’ in non-heme iron proteins from thiosulfate.
68 citations
TL;DR: It appears that the initiation and the evolution of the atherosclerotic plaque are closely associated to a proliferative reaction of the arterial wall involving both the medial smooth muscle cells and the cellular constituents of the intimal lesion.
45 citations
TL;DR: A new mandible of a Neanderthal child was found by chance by Mr Adolfo Berdar on September 6, 1970, during the opening of a motorway at the Upper Pleistocene site at Archi, near Reggio Calabria, in Italy.
Abstract: A NEW mandible of a Neanderthal child was found by chance by Mr Adolfo Berdar on September 6, 1970, during the opening of a motorway at the Upper Pleistocene site at Archi, near Reggio Calabria, in Italy.
40 citations
TL;DR: A singular perturbation method similar to that used previously for the case of oxygen shows that the absence of any observed facilitation of diffusion of this ligand by either hemoglobin or myoglobin in all of the experiments so far performed results from the very high affinity of both proteins for the gas.
36 citations
TL;DR: Kinetic models for haemoglobin based on the perfect equivalence of function of the two chains will require reconsideration in the case of some ligands, which may indicate steric effects.
34 citations
TL;DR: The ability to demonstrate pre-copulatory barriers to gene flow is demonstrated in outbreeding sexual organisms and populations can become reproductively isolated by intrinsic, heritable mechanisms operating before or after copulation.
Abstract: REPRODUCTIVE isolation is the basic process in the speciation of outbreeding sexual organisms1,2, and populations can become reproductively isolated by intrinsic, heritable mechanisms operating before or after copulation. The post-copulatory mechanisms, typically represented by hybrid sterility, can be tested by crossing experiments, but it is much more difficult to demonstrate pre-copulatory barriers to gene flow.
31 citations
TL;DR: La reale esistenza di piccoli gruppi di cellule muscolari lisce, non in rapporto with vasi, in differenti zone dell'ovaio (stroma, corpo luteo, teca, ghiandola interstiziale) del gatto, del coniglio e del topo.
Abstract: Mediante l'impiego del microscopio elettronico viene dimostrata la reale esistenza di piccoli gruppi di cellule muscolari lisce, non in rapporto con vasi, in differenti zone dell'ovaio (stroma, corpo luteo, teca, ghiandola interstiziale) del gatto, del coniglio e del topo. Le cellule muscolari subiscono caratteristiche modificazioni in relazione al ciclo ovarico.
26 citations
TL;DR: 5S and 4S RNA gene localization was studied by the in situ molecular hybridization technique in hamster liver and brain sections and hybridized “pulse-labelled” RNA appears, as expected, not to be preferentially confined in any particular nuclear structure.
22 citations
TL;DR: Evidence is presented for the formation of mixed complexes in the cases of histidine and 2-4-diaminobutyric acid, whereas a glycine-like complex with apical coordination of the δ-amino groups is proposed for the ornithine-Cu(II) complex.
TL;DR: The myoglobin contained in the buccal muscle of the Mediterranean Gasteropod Aplysia limacina shows a number of interesting functional and structural features when compared with mammalian myoglobins, and its amino acid composition and tryptic peptide pattern have been previously reported.
TL;DR: Kinetic data are presented describing interaction of pig kidney diamine oxidase with different substrates and inhibitors, and results are discussed in terms of an active site containing binding sites for the amino groups to be oxidized, for the aliphatic hydrocarbon chain of substrates, and an anionic binding site for positively charged groups at the opposite end of the molecule.
TL;DR: Ultracentrifugal examination of staphylococcal alpha toxin at different stages of purification showed the presence of a major component having a sedimentation coefficient of 2.8S, present to the extent of more than 90% of the sample, and identifiable with active toxin.
Abstract: Ultracentrifugal examination of staphylococcal alpha toxin at different stages of purification showed the presence of a major component having a sedimentation coefficient of 2.8S, present to the extent of more than 90% of the sample, and identifiable with active toxin. Several minor components having S20,w values of 11.5S, 8.5S, and 2.0S were detected. The 11.5S component presumably is identical with a toxin aggregate studied earlier and designated 12S; the 8.5S component appears to be delta toxin. A sedimentation equilibrium study of more highly purified material gave 32,700 as the best estimate of molecular weight of alpha toxin. Lowering the pH of the partially purified alpha toxin from 10.2 to 5.3 resulted in a small increase in S20,w of the 11.5S component and in the disappearance of the 8.5S component, whereas the S20,w, molecular weight, and hemolytic activity of the toxin remained constant. Exposure of toxin to pH 3.5 irreversibly reduced the S20,w to 2.0S, the molecular weight to about 16,000, and caused irreversible inactivation. Raising the pH of acid-inactivated toxin and adding sodium dodecyl sulfate to 1% increased the S20,w to near its normal value (2.7S) but did not restore activity.
TL;DR: A spectrofluorimetric investiga- tion of the binding of lucensomycin to erythrocyte ghosts is reported in a severe in- hibition of nucleic acid biosynthesis.
TL;DR: Data demonstrate that diethylstilbestrol and cyproterone acetate express their antiandrogenic effects on the testicular function, inhibiting mainly the secretion of testosterone, which is one of the most important hormonal factors in the growth of prostatic adenocarcinoma.
TL;DR: 2 cases of a familial balanced translocation of satellites and part of the short arm between two D-group chromosomes are described, one of which shows double satellites on its short arm, and the other appears deprived of visible satellites and parts of its long arm.
Abstract: 2 cases of a familial balanced translocation of satellites and part of the short arm between two D-group chromosomes are described.
TL;DR: Red blood cell adenosine deaminase and G-6-PD polymorphisms have been studied in the populations of 17 Sardinian villages located at various altitudes to confirm the negative relationship between GdMed gene frequency and altitude.
Abstract: Red blood cell adenosine deaminase and G-6-PD polymorphisms have been studied in the populations of 17 Sardinian villages located at various altitudes. A total of 1615 individuals of both sexes, with age between 7 and 14 years were examined.
TL;DR: In this article, the kinetics of paste, bottle, and ball-mill hydration of 3CaO SiO2 and the effects of additions of electrolytes and alcohols were studied.
Abstract: The kinetics of paste, bottle, and ball-mill hydration of 3CaO SiO2 and the effects of additions of electrolytes and alcohols were studied. Paste and bottle hydrations proceed through periods of induction, acceleration, and decay. If 3CaO SiO2 is hydrated in an excess of H2O, as in bottle hydration, the reaction rate is lower than that for paste hydration. The ball-mill hydration rate is much the highest and is controlled by the removal of the hydrate layer coating the 3CaO SiO2 particles. Electrolytes always accelerate and alcohols retard the reaction rate. Experimental results are discussed with reference to modern theories of the 3CaO SiO2 hydration mechanism.
TL;DR: Even in the absence of co-operative phenomena, O 2 binding by hemocyanin involves a minimum of two elementary steps, and this finding might possibly be correlated with the presence of two metal atoms in each site.
TL;DR: The EPR spectrum of ferric Aplysia myoglobin shows an axial type of high spin signal with g near 6 that changes with pH, and the p K of ionization of the heme-linked water molecule is determined from the pH dependence of this signal.
TL;DR: In OsO4-fixed ultrathin sections of the costo-chondral junctions of 1 month old guinea-pigs, Bonucci1–3 has found osmiophilic bodies with mean diameters of 500–2,500 Å, regarded as the first sites of apatite nucleation, the formation of the dark needle-like crystals in the developing matrix.
Abstract: IN OsO4-fixed ultrathin sections of the costo-chondral junctions of 1 month old guinea-pigs, Bonucci1–3 has found osmiophilic bodies with mean diameters of 500–2,500 A. The bodies are non-collagenous and contrast sharply with the surrounding matrix. He regarded them as the first sites of apatite nucleation, the formation of the dark needle-like crystals in the developing matrix.
TL;DR: In the title compound, the biacetyl bis-(N-methyl, N-phenyl)osazone acts as a tridentate ligand, the nitrogen atoms bonding via the lone pairs as discussed by the authors.
Abstract: In the title compound, the biacetyl bis-(N-methyl,N-phenyl)osazone acts as a tridentate ligand, the nitrogen atoms bonding via the lone pairs.
TL;DR: It has been demonstrated that the amount of the 300-nm absorbing compound is proportional to the initial TMA concentration and to the oxygen concentration, while is independent of the concentration of TMA-Cu II complex.
TL;DR: A controlled therapeutic trial was performed in newborns with birthweight of 750‐1 250 g, and 40 infants (the “treatment” group) received an i.v. infusion with glucose and NaHCO3 from the 1st to the 4th‐7th day of life, and increasing amounts of human milk from the 2nd day oflife.
Abstract: Summary
A controlled therapeutic trial was performed in newborns with birthweight of 750-1 250 g. 40 infants (the “treatment” group) received an i.v. infusion with glucose and NaHCO3 from the 1st to the 4th-7th day of life, and increasing amounts of human milk from the 2nd day of life. They also received, during the newborn period, 3 antibiotics (sodium penicillin G 500 000 U + methicillin 100 mg + colistin sulf. 8 mg per kg/day), a gamma globulin preparation (0.5 ml/day for 3 days) and fresh human plasma (3 ml every 2nd day in the first 5 days of birth). 40 infants (the “control” group) received only oral feeding with 10% glucose and human milk, starting in the 2nd day of life, and i.m. kanamycin (20 mg/kg/day for 5 days).
No difference between treated and control babies was observed with respect to the following findings: neonatal mortality rate and survival curve on the whole series; neonatal mortality rate in babies with birthweight above or below 1.04 kg, with gestational age below 28 weeks, with above or below median respiratory rate or arterial pH on admission, with or without marked abnormality on chest film, and in males or females (but, in subjects with gestational age above 27 weeks, a significantly lower mortality rate was found in controls); postmortem findings; incidence of apnoeic spells, tremors and cloni, and pattern of muscular tonus. Bradycardia associated with 2: 1 atrioventricular block was observed in 6 controls in the 2nd day of birth. No adverse effects presumably due to the therapeutic procedures so contrasted were demonstrated.
It was concluded that in the present series of newborns with very low weight: (a) the massive anti-infectious therapy was not superior to the administration of a single broadspectrum antibiotic; (b) the administration of fresh human plasma was not effective in preventing hemorrhage (but further evaluation is needed); (c) the routine i.v. infusion with glucose and NaHCO3 did not improve neonatal survival as compared with oral feeding.
However, previous studies, as well as observations on the present series reported elsewhere (11), have shown that several biochemical abnormalities commonly seen in orally fed newborns with very low weight can be prevented or corrected, at least in part, by the early infusion with glucose and NaHCO3. Since some of these abnormalities are potentially dangerous to the central nervous system, the final evaluation of this therapy must await long-term follow-up studies in survivors. It is therefore suggested that, before such studies are available, the early i.v. infusion with glucose and NaHCO3 should be given routinely to newborns with very low weight.
TL;DR: A case of intradural spinal lipoma has been reported and its pathological and clinical features are compared with those of 129 other cases collected from the literature.
Abstract: A case of intradural spinal lipoma has been reported. Its pathological and clinical features are compared with those of 129 other cases collected from the literature.
TL;DR: In this paper, an 18 O study of the rate of water exchange of [Ir-(NH 3 ) 5 OH 2 ] 3+ in aqueous solution is presented, and the rate constants are found to be independent of the hydrogen ion concentration in the range 0.01-0.5 M and of the ionic strength (0.96M⪕μ⪚ 3.0M).
TL;DR: Substituted 2-dichloromethyl-2H-pyrroles, when treated with strong bases under appropriate conditions, undergo ring enlargement giving substituted 3-chloropyridines as mentioned in this paper.
TL;DR: The shape of the oxidation-reduction equilibrium curve of d-amino acid oxidase is pH dependent, the value of n increasing from about 1 at pH 8.6 to about 3, or more, at pH 6.6, and one must consider the existence of functional homotropic interactions between at least 2 FAD molecules.