Showing papers by "University of São Paulo published in 2023"

Evaluation of Clinical and Biomechanical Features of Scars Resulting from the Treatment of Burn Contractures Comparing Acellular Dermal Matrices

Abstract: The aim of this study was to compare clinical and biomechanical features of scars resulting from the treatment of burn contractures using different acellular dermal matrices (ADM).Extensive burns often lead to severe sequelae, such as skin contractures, that can be treated using ADM to improve the quality of these scars.A prospective, randomized and controlled clinical trial was performed including patients with burn contractures at least 1-year post-burn, treated using split-thickness skin graft (STSG). These patients were randomized into 4 groups: control (received only STSG without ADM), Integra (ADM + STSG), Matriderm (ADM + STSG), and Pelnac (ADM + STSG). Exclusion criteria were loss of follow-up and graft integration failure. The evaluation was performed using the Vancouver Scar Scale (VSS), the durom-eter, and the cutometer in areas of normal skin, hypertrophic scar, and surgical scar, at least 1 year after the surgery.In the VSS, durometer, and cutometer evaluation, there was no difference in the comparison of surgical scars among groups. Analyzing each group, with an intraindividual evaluation comparing areas of normal skin with surgical scars, the results suggested a possibility of a surgical scar hardness closer to normal skin for Integra and Matriderm groups measured with the durometer. In the cutometer evaluation, surgical scars were not comparable to areas of normal skin.This study suggests that there is no difference in the quality of scar assessed through the analysis of clinical and biomechanical features comparing acellular dermal matrices (Integra, Matriderm, and Pelnac) and only split-thickness skin graft without a dermal matrix.

Shearless bifurcations in particle transport for reversed-shear tokamaks

Abstract: Some internal transport barriers in tokamaks have been related to the vicinity of extrema of the plasma equilibrium profiles. This effect is numerically investigated by considering the guiding-centre trajectories of plasma particles undergoing $\boldsymbol {E}\times \boldsymbol {B}$ drift motion, considering that the electric field has a stationary non-monotonic radial profile and an electrostatic fluctuation. In addition, the equilibrium configuration has a non-monotonic safety factor profile. The numerical integration of the equations of motion yields a symplectic map with shearless barriers. By changing the safety factor profile parameters, the appearance and breakup of these shearless curves are observed. The shearless curve's successive breakup and recovery are explained using concepts from bifurcation theory. We also present bifurcation sequences associated with the creation of multiple shearless curves. Physical consequences of scenarios with multiple shearless curves are discussed.

Effects of omega-3 supplementation on anxiety-like behaviors and neuroinflammation in Wistar rats following cafeteria diet-induced obesity

Abstract: ABSTRACTObjetives: Omega-3 (n3) fatty acids have been studied as an option to alleviate the harmful effects of obesity. However, its role in obesity-related behavioral changes is still controversial. This study aimed to evaluate the effects of n3 on behavior and neuroinflammation in obese animals. Methods: Male Wistar rats were divided into four groups: control diet (CT), CT+n3, cafeteria diet (CAF), and CAF+n3. Diet was administered for 13 weeks, and n3 was supplemented during the last 5 weeks. Metabolic and biochemical parameters were evaluated, as well as anxiety-like behaviors. Immunoblots were conducted in the animals' cerebral cortex and hippocampus to assess changes in neuroinflammatory markers.Results: CAF-fed animals showed higher weight gain, visceral adiposity, fasting glucose, total cholesterol, triglycerides, and insulin levels, and n3 improved the lipid profile and restored insulin sensitivity. CAF-fed rats showed anxiety-like behaviors in the open field and light-dark box tasks but not in the contextual aversive conditioning. Omega-3 did not exert any effect on these behaviors. Regarding neuroinflammation, diet and supplementation acted in a region-specific manner. In the hippocampus, CAF reduced claudin-5 expression with no effect of n3, indicating a brain-blood barrier disruption following CAF. Furthermore, in the hippocampus, the glial fibrillary acidic protein (GFAP) and toll-like receptor 4 (TLR-4) were reduced in treated obese animals. However, n3 could not reverse the TLR-4 expression increase in the cerebral cortex.Discussion: Although n3 may protect against some neuroinflammatory manifestations in the hippocampus, it does not seem sufficient to reverse the increase in anxiolytic manifestations caused by CAF.

Integrative toolbox to assess the quality of freshwater sediments contaminated with potentially toxic metals

Abstract: The Guadiana Basin is a transnational basin, presenting historical contamination with potentially toxic metals (PTM), which origin can be both natural and anthropogenic. This study explores the use of a set of observational, chemical and ecotoxicological assays with Heterocypris incongruens, Vibrio fischeri, Pseudokirchneriella subcapitata, Thamnocephalus platyurus, identifying the most sensitive to be included in a toolbox to analyze the quality of freshwater sediments related to this type of contamination. The study included the analysis of a reservoir and streams sediments of Guadiana basin, in two consecutive years with different climate conditions 2017 (dry year) and 2018 (normal year). The results showed high chemical variability along the basin, with greater contamination with PTM in the reservoir sediments. The calculated Enrichment Factors (EF) indicated high anthropogenic contamination by Cd, followed by Pb (EF > 1.5). The geoaccumulation index (Igeo) revealed that the sediments were severely polluted with Cd, and slightly polluted with Pb and Cu, inducing a higher sublethal toxicity to Heterocypris incongruens. Among the parameters evaluated, and after the use of multivariate statistical techniques, the toolbox for assessing sediments quality, in similar climate and geological conditions, should include the analysis of: meteorology, land use/cover in the area, granulometry, organic matter content, PTM concentrations, contamination indices (e.g., Igeo and EF), and sublethal bioassays with H. incongruens (total sediment analysis) and Vibrio fisheri luminescence inhibition (pore water analysis).

Positive Ricci curvature through Cheeger deformations

Abstract: This paper is devoted to a deep analysis of the process known as Cheeger deformation, applied to manifolds with isometric group actions. Here, we provide new curvature estimates near singular orbits and present several applications. As the main result, we answer a question raised by a seminal result of Searle–Wilhelm about lifting positive Ricci curvature from the quotient of an isometric action. To answer this question, we develop techniques that can be used to provide a substantially streamlined version of a classical result of Lawson and Yau, generalize a curvature condition of Chavéz, Derdzinski, and Rigas, as well as, give an alternative proof of a result of Grove and Ziller.

The impact of coffee origin information on sensory and hedonic judgment of fine Amazonian robusta coffee

Abstract: Through the lens of predictive processing theory, perception is shaped by expectations we create about object information, including food products, and their match/mismatch with sensory stimuli. The popularity of labelling origin information to enhance product value has been spread to high-quality foods, particularly as geographical indication (GI). The question we propose is whether the coffee's origin information can affect the sensory perception and hedonic judgment given by professional coffee tasters. Participants were divided into groups according to GI cue provision (absent, GI cue, GI storytelling) associated to the coffee sample served. Origin information proved to significantly affect participants' sensory perception, but not hedonic judgment. Precisely, participants judged acidity and overall flavor as more intense after reading the story whereas higher ratings of nutty, cacao, and caramel notes were given when origin was absent. It is likely that, origin and sorytelling influenced participants' expectations and sensory perception. Oppositely, a better perception of flavor notes was observed only in the absence of information. Practical applications This study contributes to the literature of food marketing by showing that professional coffee tasters' sensory evaluations of specialty coffee are affected by product origin and how it is communicated. These findings emphasize the relevance of origin cues in food marketing and their potential on influencing expectations and sensory perception. Coffee companies and brands can benefit from communicating the origin of specialty coffee by building strategies on place reputation and image. Considering that the specialty coffee industry is growing fast and offers a variety of product options (e.g., organic, fair trade, award winner), new coffee varieties and recently registered GIs can disclose the origin cue to capture consumers' attention and enhance product value by referring to the uniqueness of a region. Additionally, different coffee profiles could be matched with different presentations of the origin cue in order to potentially enhance the consumers' perception of the beverage's attributes, improving the drinking experience.

Evaluation of the clinical performance of dentures manufactured by computer-aided technology and conventional techniques: A systematic review

Abstract: The introduction of computer-aided design and computer-aided manufacturing (CAD-CAM) technology for complete denture fabrication may have improved clinical outcomes compared with conventional techniques. However, systematic reviews comparing these techniques are lacking.The purpose of this systematic review was to identify, compare, and synthesize the outcomes of published clinical studies related to complete denture fabrication, with respect to the differences between CAD-CAM technology and conventional techniques.A comprehensive search of studies published up to March 16, 2020, was conducted by using the PubMed/MEDLINE, Web of Science, Cochrane Library, SciELO, and Embase databases according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement criteria and was registered in the International Prospective Register of Systematic Reviews (PROSPERO ID 42020202614). The population, intervention, comparison, and outcome (PICO) question was: Do CAD-CAM complete dentures have a similar functional performance to those fabricated by conventional techniques? The quality of publications was appraised by using the Critical Appraisal Skills Program (CASP) checklists.Of the 1232 titles, 6 articles were selected. The studies reported better retention of digitally manufactured complete dentures without denture adhesives than that of conventional complete dentures with or without denture adhesives. Other studies reported that dentures manufactured with digital systems were better adapted to tissue surfaces, required less clinical time, were lower in cost, and provided better experience and satisfaction to patients.The assessment of CAD-CAM planning and manufacturing through clinical studies is ongoing. However, preliminary results indicate better clinical performance and lower overall costs of digital complete dentures than conventional dentures.

Effect of Photobiomodulation With Different Wavelengths on Radiodermatitis Treatment

Abstract: Background: Approximately 80% of patients submitted to radiotherapy develop radiodermatitis. Photobiomodulation based on light-emitted diode (LED) is one of the therapeutic strategies for treating inflammation. This study aimed to investigate the effect of the photobiomodulation with two wavelengths, in an acute radiodermatitis animal model. Methods: Twenty rats were submitted to one radiotherapy session. After 15 days, the rats that developed radiodermatitis were divided into control groups, LED-630 nm, LED-850 nm, and LED-630 + 850 nm. The treatment regimen was one session lasting 10 minutes on alternate days for 21 days. We analyzed macroscopy aspects (RTOG scale), vascular density, dermal appendages, VEGF-a, TNF-alpha, MMP-9, and MMP-9 genic expression level. Results: All LED groups revealed a two-point reduction on the radiodermatitis severity grade compared with the baseline classification. Dermal appendage and vascular analysis showed a higher counting in all LED groups compared to control. This study showed dermal appendages twice in the 630/850 nm group compared with the control group. The 630/850 nm group showed six times more arterioles than the control group. Regarding genic expression, this study showed a 10-fold decrease between LED-630 nm versus LED-630 + 850 nm (P = 0.02) interleukin-10 expression and a 12-fold decrease between control versus LED-630 nm (P = 0.006) and LED-850 nm (P = 0.002) in TNF-alpha. Conclusion: LED (630 nm, 850 nm, and 630 nm + 850 nm) showed benefit in the treatment of radiodermatitis, and the association of the 630 nm + 850 nm and 630 nm parameters demonstrated the best macroscopic and microscopic results.

Alternate morphological pathways involved in climbing ability in ten Neotropical anuran species

Abstract: Abstract Convergent evolution has been shown to be a prominent feature of anuran evolutionary history. Studying the morphological pathways involved in the evolution of a convergent character allows us to test whether deterministic or contingent forces drive the evolution of characters. Here, we have assessed the morphological pathways associated with arboreal habits in species of six families of anurans (Hylidae, Eleutherodactylidae, Strabomantidae, Centrolenidae, Bufonidae and Hemiphractidae) through a comparative analysis of 19 phenotypic characters related to climbing ability. All species showed differences in the assessed characters, exhibiting variations in the distribution of their states and different ranges in all limb lengths. These variations implied a wide distribution across the morphospace as defined by a non-metric multidimensional scaling analysis (NMDS), with Rhinella paraguas (Bufonidae) being the most distinctive species, presenting unique characters such as the absence of intercalary elements, adhesive pads, subarticular tubercles and interphalangealis muscles of the hands. Our findings demonstrate that the morphological mechanisms involved in climbing ability of the ten species are different, suggesting distinct morphological pathways. Consequently, we advocate that historical contingency has an essential role in the evolution of arboreal habits among the species studied.

Can hyper/hypo‐osmoregulating fiddler crabs from the Atlantic coast of South America mobilize intracellular free amino acids as osmotic effectors during salinity challenge?

Abstract: Weakly osmoregulating crustaceans use intracellular free amino acids (FAA) to attenuate cell volume changes consequent to alterations in hemolymph osmolality. Whether semiterrestrial, strong hyper/hypo-osmoregulators exhibit this ability is unknown. We investigate FAA mobilization in muscle tissue of 10 fiddler crabs from the genera Minuca, Leptuca, and Uca distributed along the Atlantic coast of South America. Crabs were subjected to severe hypo- or hyper-osmotic challenge at their lower or upper critical salinity limits for 5 days; reference crabs were held in isosmotic media. Hemolymph osmolality was measured, chela muscle FAA were identified and quantified, and percent contribution to intracellular osmolality (%FAA) was calculated. At isosmoticity, total FAA were nominally twofold higher in Minuca species (≈116 mmol/kg wet mass) compared to Uca (≈60 mmol/kg wet mass). Glycine, alanine, arginine, and taurine constituted >80% of the total FAA pool. On hyperosmotic challenge, hemolymph osmolalities ranged from 843 to 1282 mOsm/kg H2O. FAA increased, although %FAA remained unaltered. Hypo-osmoregulating crabs thus can mobilize FAA, likely owing to a lesser ability to secrete salt near their upper critical limits. On hypo-osmotic challenge, osmolalities were regulated more tightly, between 475 and 736 mOsm/kg H2O. Total FAA and %FAA showed little change, probably due to the crabs' strong hyper-osmotic extracellular regulatory ability, FAA consequently playing a diminished role in isosmotic intracellular regulation (IIR). Total FAA responses to hyper/hypo-osmotic challenge are thus asymmetrical. The lack of phylogenetic signal in FAA mobilization suggests that closely related fiddler crabs do not share similar strategies of IIR.

Combining IP3 affinity chromatography and bioinformatics reveals a novel protein-IP3 binding site on Plasmodium falciparum MDR1 transporter

Abstract: Intracellular Ca2+ mobilization induced by second messenger IP3 controls many cellular events in most of the eukaryotic groups. Despite the increasing evidence of IP3-induced Ca2+ in apicomplexan parasites like Plasmodium, responsible for malaria infection, no protein with potential function as an IP3-receptor has been identified. The use of bioinformatic analyses based on previously known sequences of IP3-receptor failed to identify potential IP3-receptor candidates in any Apicomplexa. In this work, we combine the biochemical approach of an IP3 affinity chromatography column with bioinformatic meta-analyses to identify potential vital membrane proteins that present binding with IP3 in Plasmodium falciparum. Our analyses reveal that PF3D7_0523000, a gene that codes a transport protein associated with multidrug resistance as a potential target for IP3. This work provides a new insight for probing potential candidates for IP3-receptor in Apicomplexa.

Controlled release urea increases soybean yield without compromising symbiotic nitrogen fixation

Abstract: Summary In Brazil, high-yield soybean [ Glycine Max (L). Merrill] – corn ( Zea mays L.) double cropping system might be nitrogen (N)-limited and additional N fertilization can be beneficial. Early application of N in soybean reduces the symbiotic N fixation (SNF) efficiency and/or establishment. One alternative to avoid SNF impairment is to apply N between the beginning pod (R3) and seed-fill (R5) stages through the use of controlled release fertilizers. In this study, N was applied at 50 kg ha −1 as common urea (CU) or controlled release urea (CRU) with different lag periods until N release starts (30 days, 60 days, or 1:1 mix of both lag times) in a randomized complete blocks design with six treatments and four replicates under tropical and subtropical conditions. CU was applied after soybean emergence (VE) or at the beginning pod (R3), and CRU only at VE. Using urea labeled with 15 N isotope, we analyzed the N source used by soybean (fertilizer, soil, or SNF) and SNF parameters. On average, CRU – 30 days, CRU – 1:1 mix (30 + 60 days) and CU applied at the R3 stage increased grain yield by 9.2% (354 kg ha −1 ) compared to the control. N derived from all fertilizer treatment were almost 35 kg N ha −1 , a high N recovery efficiency of 68%. The SNF was not impaired by CU and CRU and accounted for 71% (220 kg N ha −1 ) of total N uptake. In the conditions of the experiments, fertilization of 50 kg N ha −1 as CRU was shown to be effective to supply N in late soybean demand (R3 stage), increasing yield without damaging the SNF process in high-yield environments.

Electrochemical Nanomaterial-based Sensors/Biosensors for Drug Monitoring

Abstract: Determining the amount of medication used is essential for correctly managing treatment systems. The unauthorized use of drugs and the importance of determining the absorbed and required dose of drugs in target organs are essential factors that justify the design of new drug monitoring systems. Electrochemical sensors and biosensors based on nanomaterials have been developed for drug monitoring in the past few years. The use of nanomaterials to optimize the analyte detection process and facilitate electron transfer in electrochemical processes has enhanced intermolecular interactions and increased diagnostic sensitivity. Considering this review, in the first part, the evaluation of cancer drugs is examined, which can be used to determine the exact dose of the drug required in different stages of cancer. Accurate monitoring of cancer drugs can increase patient life expectancy, reduce side effects, and increase economic savings. In the next section, sensors and biosensors designed for antibiotics are examined. Accurate measurement of antibiotics for determining the effectiveness of the dose in controlling infections and preventing antibiotic resistance is possible with the help of these drug diagnostic platforms. In the next part, the diagnosis of different hormones is considered. Abnormal amounts (low/high) of hormones cause multiple physiological complications and various disabilities. Therefore, accurate determination of hormone levels can effectively treat hormonal changes. In the last section, other drugs, including drugs and analgesics for which the use of electrochemical diagnostic platforms can significantly help drug distribution and social health systems, are also discussed.

Factors Associated with Spontaneous Clearance of Recently Acquired Hepatitis C Virus among HIV-Positive Men in Brazil

Abstract: The objective of the present study was to describe the clinical and epidemiological aspects of recently acquired hepatitis C virus (HCV) infection and the frequency of its spontaneous clearance in a people living with the human immunodeficiency virus (PLWH) cohort.We reviewed the medical records from all PLWH at the human immunodeficiency virus (HIV) outpatient reference clinic affiliated with the University of São Paulo, Brazil, and identified, by immunoassays and RNA-PCR individuals who acquired HCV infection between January 2015 and December 2017. The factors associated with subsequent spontaneous clearance of the infection in this group were identified and analyzed.Among 3143 PLWH individuals, 362 (11.5%) were coinfected with HCV. Forty-eight (13.2%) of these subjects first became HCV-positive between January 2015 and December 2017. Spontaneous HCV clearance was documented in 23 individuals (47.9%). The majority of this latter group were male (83.3%), and the median age was 31 years (23-39). The main risk group for HCV acquisition was men who had sex with men (MSM) (89.5%). In a multivariate analysis, only an elevated CD4+ T lymphocyte count at the time of seroconversion was found to be associated with subsequent HCV clearance (p = 0.025).In HIV-infected individuals in Sao Paulo, Brazil, most cases of recent HCV transmission were by sexual exposure. In PLWH, particularly in MSM, the individual's CD4+ T lymphocyte count is a determinant of whether an acquired HCV infection will be prolonged or will spontaneously clear.

The Contradictions in Vladimir Putin's "Just War" against Ukraine: The Myths of NATO’s Containment, Minority Protection and Denazification

Abstract: Since Russia's full-scale invasion of Ukraine in February 2022, Putin has mobilized different arguments to justify his bellicose stance, mainly the pursuit of NATO containment, the protection of ethnic Russians and Russian-speaking minorities, and the "denazification" of Ukraine. In this article I analyze the contradictions and inconsistencies in these three arguments. Firstly, I show that, although Russian elites had legitimate considerations to contest NATO enlargement during the post-Cold War era, there is no evidence that Putin’s military aggression was motivated by this issue: as previously expected, the invasion has further strengthened the alliance, provided it with a legitimate raison d'être, and contributed to an even more intense military presence near Russian borders; the European balance of power is now much more favorable to NATO than before the conflict. Secondly regarding the humanitarian intentions of protecting the Russian-speaking communities in Eastern Ukraine, the Russian invasion has so far led to the opposite result: ten months of war caused more civilian deaths than eight years of war in Donbas, and the Russian-speaking population has been exactly the most affected victim. Finally, with regard to the "fight against Nazism", although in Ukraine there are ultranationalist groups, there is no evidence that such groups currently count on significant political influence and broad support in society; furthermore, the Russian authorities themselves have ties to similar groups. The "denazification" narrative represents a strategy of demonizing the opponent, mobilized to legitimize the dismemberment and extinguishing of Ukraine as a state and nation. The current war has laid bare the Kremlin's double standard politics: civilian deaths resulting from attempts by Ukraine to regain control of its territory are evidence of "genocide"; while the civilian deaths resulting from the Russian invasion are just the "side effects" of a legitimate fight against "Nazis" and NATO.

The rs2682826 Polymorphism of the NOS1 Gene Is Associated with the Degree of Disability of Erectile Dysfunction

Abstract: Erectile dysfunction (ED) is a common male disorder, often associated with cardiovascular disease and ageing. The Sildenafil, a PDE5 inhibitor, can improve the erectile function by prolonging the nitric oxide (NO) downstream effect. NO is a molecule of pivotal importance in erection physiology and is mainly produced by neuronal nitric oxide synthase (nNOS) and endothelial NO synthase (eNOS). While it has been shown that eNOS and nNOS genetic polymorphisms could be associated with Sildenafil responsiveness in ED, no study so far has assessed whether nNOS polymorphisms and PDE5A polymorphism could be associated with increased risk to ED or with intensity of symptoms. A total of 119 ED patients and 114 controls were studied, with evaluation of the clinical disability by the International Index for Erectile Function instrument, plasma assessment of nitrite levels and genomic DNA analysis regarding the rs41279104 and rs2682826 polymorphisms of the NOS1 gene and the rs2389866, rs3733526 and rs13124532 polymorphisms of the PDE5A gene. We have found a significant association of the rs2682826 with lower IIEF scores in the clinical ED group. While this result should be confirmed in other populations, it may be helpful in establishing a genetic panel to better assess disease risk and prognosis on ED therapy.

There and back again: when and how the world’s richest snake family (Dipsadidae) dispersed and speciated across the Neotropical region

Abstract: ABSTRACT Aim The widespread megadiverse Neotropical snake family Dipsadidae occurs in a large range of diverse habitats. Thus it represents an excellent model to study the diversification of Neotropical biota. Herein, by generating a time-calibrated species-level phylogeny, we investigate the origin and historical biogeography of Dipsadidae and test if its two main Neotropical subfamilies, Xenodontinae and Dipsadinae, have different geographical origins. Location Neotropical region. Taxon Dipsadidae (Serpentes). Methods We generated a new Bayesian time-calibrated phylogeny including sequences from six genes for 344 species, including 287 species of Dipsadidae. We subsequently estimated ancestral areas of distribution by comparing models in BioGeoBEARS: DEC (subset sympatry, narrow vicariance), DIVALIKE (narrow and wide vicariance), BAYAREALIKE (no vicariance and widespread sympatry), also testing jump dispersal. Results The best models show that Dipsadidae likely originated approximately 50 million years ago (mya) in Asia. Dispersal was a fundamental process in its historical biogeography. The DEC model with jump dispersal indicated that this family underwent a range extension from Asia and posterior vicariance of North and Central America ancestors. Both Xenodontinae and Dipsadinae originated in Central America and dispersed to South America during Middle Eocene, but did so to different regions (cis and trans-Andean South America, respectively). Xenodontinae entered cis-Andean South America around 39 mya and jump dispersed to the West Indies around 33 mya, while Dipsadinae entered trans-Andean South America multiple times 20 – 38 mya. Main conclusions Our results show that Dipsadidae has an Asian origin and that the two main Neotropical subfamilies originated in Central America, later dispersing to South America in different time periods. The current biogeographical patterns of the family Dipsadidae, the most species-rich snake family in the world, have likely been shaped by complex evolutionary and geological processes such as Eocene land bridges, Andean uplift and the formation of the Panama isthmus.