Showing papers by "University of Texas Health Science Center at Houston published in 1970"
TL;DR: A microcolony technique for assaying the survival of small proportions of the clonogenic cells of the mucosa of the small intestine of mice is described.
Abstract: SummaryA microcolony technique for assaying the survival of small proportions of the clonogenic cells of the mucosa of the small intestine of mice is described. The radiosensitivity of intestinal crypt cells measured by the technique is the same as that measured by a macrocolony method. However, the microcolony technique has the special advantage of being useful for assaying cell survival after whole-body radiation.
685 citations
TL;DR: No positive correlation between the degree of growth hormone elevation and the abnormality of glucose tolerance was observed, and the carbohydrate intolerance and hyperinsulinism associated with uremia must however be attributed to factors other than growth hormone alone.
Abstract: Fasting plasma growth hormone was 6 mμg./ml. or more in 13 of 21 patients with severe renal failure. Growth hormone levels of control subjects regularly decreased following an intravenous infusion of glucose. In contrast, the growth hormone levels of only three of 21 uremic patients were suppressed by glucose, eleven of the patients exhibiting a paradoxical rise. We observed no positive correlation between the degree of growth hormone elevation and the abnormality of glucose tolerance. Growth hormone levels were also measured at four hourly intervals during hemodialysis. Increasing the glucose concentration of dialysate did not noticeably modify the growth hormone response to hemodialysis in these patients. The pathologic significance of increased growth hormone in uremia is unknown. The carbohydrate intolerance and hyperinsulinism associated with uremia must however be attributed to factors other than growth hormone alone.
154 citations
143 citations
TL;DR: Two new species of Pseudomonas are described: one, a yellow-pigmented hydrogen bacterium, P. palleronii Davis; the other, a non-autotrophic, non- pigmented species,P.
Abstract: The properties of several named and unnamed strains of polarly flagellated “hydrogen bacteria” are described and compared with those of related autotrophic and non-autotrophic species Two new species ofPseudomonas are described: one, a yellow-pigmented hydrogen bacterium,P palleronii Davis; the other, a non-autotrophic, non-pigmented species,P delafieldii Davis
88 citations
TL;DR: It is proposed that all mutants blocked in the de novo pathway of nicotinamide adenine dinucleotide biosynthesis be designated nad rather than nic and mutants blocking in the pyridine nucleotide cycle be designated pnc.
Abstract: It is proposed that all mutants blocked in the de novo pathway of nicotinamide adenine dinucleotide biosynthesis be designated nad rather than nic It is further suggested that mutants blocked in the pyridine nucleotide cycle be designated pnc The nadB locus and a previously unidentified pur locus are cotransducible These two loci have been mapped near minute 49 on the standard genetic map of Escherichia coli The order of genes in that region is purC-guaB-purG-glyA-pur-nadB-tyrA-pheA
45 citations
39 citations
TL;DR: This computer program provides a characterization of a set of survival time data in terms of three survival functions, each of which illustrates a different aspect of the data.
24 citations
TL;DR: An electron microscopic investigation of human gingiva and rat oral mucosa revealed the presence of solitary cilia primarily in the germinative layer of the epithelium that lacked the central fibrils normally found in motile cilia.
Abstract: An electron microscopic investigation of human gingiva and rat oral mucosa revealed the presence of solitary cilia primarily in the germinative layer of the epithelium. The cilia were infrequently found in the stromal cells of the lamina propria. These cilia were associated with the diplosome but lacked the central fibrils normally found in motile cilia.
18 citations
Journal Article•
TL;DR: Adoptive immunity occurred more frequently in recipients with granulocytic leukemia than in recipient with solid tumors of lymphoma, and leukocytes from both types of donors were equally successful at transferring immunity.
Abstract: Adoptive immunity in man has been studied with the primary antigens of keyhole limpet hemocyanin (KLH) and the amino acid copolymer of the l-isomers of glutamic acid, lysine, alanine, and tyrosine (GLAT). Thirteen volunteer donors with chronic lymphocytic leukemia and other malignant diseases were immunized with KLH or GLAT or both. Immune peripheral blood leukocytes were collected with an IBM-NCI blood cell separator. Between 1.1 and 15.6 × 1010 lymphocytes were collected from the chronic lymphocytic leukemia leukocyte donors; from 0.16 to 1.1 × 1010 lymphocytes were collected from the other donors. The leukocyte recipients were all patients with advanced malignant disease. Thirty-one of the 55 recipients evaluated for KLH sensitivity after the transfusion had evidence of immunity as measured by delayed hypersensitivity, antibody formation, or in vitro lymphocyte blastogenesis. Eleven of 13 studied for transfer of GLAT immunity were positive by at least one of the above parameters of immunity. Ten of 46 recipients skin tested were KLH skin test positive, as was 1 of 7 skin tested for GLAT. Ten of 47 and 5 of 13 recipients whose lymphocytes were evaluated for in vitro lymphocyte blastogenesis stimulated by KLH and GLAT, respectively, were positive. Fifty-eight % of 36 recipients evaluated developed KLH antibody, compared with 11 of 13 leukocyte recipients producing GLAT antibody. Leukocytes from both types of donors were equally successful at transferring immunity. Adoptive immunity occurred more frequently in recipients with granulocytic leukemia than in recipients with solid tumors of lymphoma.
16 citations
TL;DR: Eine Bestimmungsmethode für die Interaktion zwischen Histon and Nukleinsäuren (RNS oder DNA) wurde beschrieben, die Hemmung der RNS-Synthese in vitro mit Histon, die unabhängig von der Gegenwart der R NS im Reaktionsgemisch ist, erklärt.
Abstract: Eine Bestimmungsmethode fur die Interaktion zwischen Histon und Nukleinsauren (RNS oder DNA) wurde beschrieben. Beide Nukleinsauren komplexieren mit Histon; RNS-Histonkomplexe sind schwach und dissozieren in Gegenwart von DNS. Damit wird die Hemmung der RNS-Synthese in vitro mit Histon, die unabhangig von der Gegenwart der RNS im Reaktionsgemisch ist, erklart.
11 citations
TL;DR: Three analog signals, instantaneous air-flow, O 2 and CO 2 concentration in inspired and expired gas, are transmitted by telephone line to a hybrid computer system, digitized and processed by on-line computer.
TL;DR: Bacterionema matruchotii, an oral filamentous organism, dissociated to form unusual flat colonies that yielded pure cultures of bacillary and streptococcal variants.
Abstract: Bacterionema matruchotii, an oral filamentous organism, dissociated to form unusual flat colonies Subculture of the flat colonies, composed of diphtheroids, yielded pure cultures of bacillary and streptococcal variants
TL;DR: A functionally balanced occlusion with facial balance and harmony was produced by anterior maxillary ostectomy, genioplasty, and short-term orthodontic treatment.
TL;DR: The most important changes in surviving cells of the irradiated cultures were the formation of multinucleated giant cells, cellular vacuolization, and movements of the cell membranes (zeiosis and pericyclosis).
Abstract: Cultures of oral mucosa from 15- to 17-day-old rat fetuses were placed under cellophane membranes on cover glasses of Rose chambers to determine the action of low doses of X radiation on the oral epithelial cells, exclusive of mitotic phenomena. The principal characteristic of the emigrating cells in control cultures was established. The most important changes in surviving cells of the irradiated cultures were the formation of multinucleated giant cells, cellular vacuolization, and movements of the cell membranes (zeiosis and pericyclosis). Even with the low doses, there was a decided inhibition of growth.