University of the District of Columbia

About: University of the District of Columbia is a education organization based out in Washington D.C., District of Columbia, United States. It is known for research contribution in the topics: Population & Spintronics. The organization has 642 authors who have published 1192 publications receiving 30025 citations. The organization is also known as: UDC & Federal City College.

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Abstract: In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure flux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation, it is imperative to target by gene knockout or RNA interference more than one autophagy-related protein. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways implying that not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular assays, we hope to encourage technical innovation in the field.

Black students' school success: Coping with the “burden of ‘acting white’”

Abstract: The authors review their previous explanation of black students' underachievement. They now suggest the importance of considering black people's expressive responses to their historical status and experience in America. “Fictive kinship” is proposed as a framework for understanding how a sense of collective identity enters into the process of schooling and affects academic achievement. The authors support their argument with ethnographic data from a high school in Washington, D.C., showing how the fear of being accused of “acting white” causes a social and psychological situation which diminishes black students' academic effort and thus leads to underachievement. Policy and programmatic implications are discussed.

An optimal algorithm for approximate nearest neighbor searching fixed dimensions

Abstract: Consider a set of S of n data points in real d-dimensional space, Rd, where distances are measured using any Minkowski metric. In nearest neighbor searching, we preprocess S into a data structure, so that given any query point q∈ Rd, is the closest point of S to q can be reported quickly. Given any positive real ϵ, data point p is a (1 +ϵ)-approximate nearest neighbor of q if its distance from q is within a factor of (1 + ϵ) of the distance to the true nearest neighbor. We show that it is possible to preprocess a set of n points in Rd in O(dn log n) time and O(dn) space, so that given a query point q ∈ Rd, and ϵ > 0, a (1 + ϵ)-approximate nearest neighbor of q can be computed in O(cd, ϵ log n) time, where cd,ϵ≤d ⌈1 + 6d/ϵ⌉d is a factor depending only on dimension and ϵ. In general, we show that given an integer k ≥ 1, (1 + ϵ)-approximations to the k nearest neighbors of q can be computed in additional O(kd log n) time.

Vehicular ad hoc networks (VANETS): status, results, and challenges

Abstract: Recent advances in hardware, software, and communication technologies are enabling the design and implementation of a whole range of different types of networks that are being deployed in various environments. One such network that has received a lot of interest in the last couple of years is the Vehicular Ad-Hoc Network (VANET). VANET has become an active area of research, standardization, and development because it has tremendous potential to improve vehicle and road safety, traffic efficiency, and convenience as well as comfort to both drivers and passengers. Recent research efforts have placed a strong emphasis on novel VANET design architectures and implementations. A lot of VANET research work have focused on specific areas including routing, broadcasting, Quality of Service (QoS), and security. We survey some of the recent research results in these areas. We present a review of wireless access standards for VANETs, and describe some of the recent VANET trials and deployments in the US, Japan, and the European Union. In addition, we also briefly present some of the simulators currently available to VANET researchers for VANET simulations and we assess their benefits and limitations. Finally, we outline some of the VANET research challenges that still need to be addressed to enable the ubiquitous deployment and widespead adoption of scalable, reliable, robust, and secure VANET architectures, protocols, technologies, and services.