Showing papers in "Analytical Biochemistry in 1982"

TL;DR: A new automated system for the analysis of nitrate via reduction with a high-pressure cadmium column that automatically eliminates interference from other compounds normally present in urine and other biological fluids is described.

TL;DR: A sensitive silver stain for detecting bacterial lipopolysaccharides in polyacrylamide gels is developed by modifying the silver-staining method used for proteins, which is 500 times more sensitive than the periodic acid-Schiff stain and can detect less than 5 ng of rough type lipopolySaccharides.

TL;DR: A procedure for the assay of antibodies in sera based on the application of the antigen as a spot to nitrocellulose filters is described, which has the merit of being simpler in operation and more sensitive than comparable existing procedures.

TL;DR: A new solid-state reagent for iodinating polypeptides and proteins was used to radiolabel antiporcine insulin antiserum which retained its immunological ability to recognize insulin.

TL;DR: A new colorimetric technique for the estimation of ascorbic acid by using Folin phenol reagent has been developed and has been found to be stable up to 18 h and almost 100% efficient.

TL;DR: Zeta-bind, a positively charged nylon membrane, was tested as an immobilizing matrix for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels and found that Zeta- bind has a considerably greater capacity than does nitrocellulose for protein binding.

TL;DR: A method is described for the detection of concanavalin A-binding proteins in complex mixtures where the proteins are separated by gel electrophoresis and then blotted onto a nitrocellulose sheet where they form an immobilized replica of the original gel.

TL;DR: A highly sensitive silver stain for visualizing proteins in polyacrylamide gels was adapted for staining 1,2-diol groups of carbohydrates, and its lower limit of detection is comparable to autoradiography of radioactively labeled lipopolysaccharides.

TL;DR: A rapid and simple gas-liquid chromatographic method for the determination of subnanomolar amounts of carbohydrates derived from glycoproteins is described, applicable to samples containing uronic acids and lipids.

TL;DR: In the present method, all detectable RNA, chromosomal DNA, and protein are removed without the use of enzymes, phenol extraction, dialysis, or equilibrium centrifugation.

TL;DR: This spin label is useful as a sensitive thiol titrating agent as well as a specific conformational probe of thiol site structure by virtue of its minimal rotational freedom and distance from the covalent disulfide linkage to the macromolecule under study.

TL;DR: The combination of the powerful chromatographic technology and the ability to predict the elution positions of peptides based on their composition provides a very potent method for the isolation of peptide from biological tissues.

TL;DR: A simple, rapid procedure for obtaining partial peptide maps from nanogram quantities of protein in gel slices using the selective tryptophanyl peptide bond cleavage reagent N-chlorosuccinimide/urea is presented.

TL;DR: Use of isopropanol improved activity staining of RNases with each of the 10 SDS preparations examined, and reduced the variability from preparation to preparation observed when buffer alone was used to remove SDS.

TL;DR: RNA and single-stranded DNA species as short as 10 to 20 nucleotides were detected with high sensitivity after electrophoresis on denaturing gels containing urea, suggesting that silver staining may be applicable to the sequencing of a few micrograms of unlabeled DNA.

TL;DR: Glycoproteins in nitrocellulose transfers of electrophoretically separated mixtures of cellular and viral proteins are rapidly and sensitively located by sequential incubation with the lectin concanavalin A and the enzymatically active glycoprotein horseradish peroxidase.

TL;DR: Continuous electrophoresis buffers are described for polyacrylamide gels at pH values ranging from 3.8 to 10.2 and are likely to be useful in the detection of genetic variation in proteins and in other applications.

TL;DR: The procedure has been used to prepare DNA from another filamentous basidiomycete, Heterobasidion annosum, and from yeast and with slight modification the procedure is attractive for isolating recombinant plasmids from yeast.

TL;DR: Practical examples of the performance of the combined trifluoroacetate/acetate buffer with Ultrasphere octadecylsilane gives optimal separations and peak sharpness in low-nanomole NH2-terminal sequence analysis.

TL;DR: Data support the hypothesis that, as in mice, the particular cytosol binding species in rats is the receptor that may mediate the induction of aryl hydrocarbon hydroxylase activity.

TL;DR: Theoretical plate values determined for small, unretained molecules are found to be noncorrelative to protein resolution, and a test mixture is proposed for comparing the ability of commercial columns to resolve proteins.

TL;DR: A rapid, reproducible, and relatively inexpensive method for quantitative analysis of two-dimensional gel radiofluorograms and can be used to quantitate protein- or DNA-stained one- or two- dimensional gels or chromatograms.

TL;DR: The extracellular chitinase produced by Serratia marcescens was obtained in highly purified form by adsorption-digestion on Chitin this article.

TL;DR: The chromatographic behavior of over 35 naturally occurring nucleotides in this system is described which permits tentative assignment of most of the peaks observed in HPLC-uv profiles of cell extracts and provides an evaluation of potential sources of error in the analysis of nucleotide pool sizes.

TL;DR: A rapid and convenient method is, therefore, now available for multiple protein determinations following sucellular fractionation on sucrose gradients.

TL;DR: It is found that the addition of a predetermined volume of buffered dye solution to each sample is the only manipulation required prior to fluorescence measurement makes this procedure the simplest yet described for quantitating DNA collected during alkaline elution.

TL;DR: The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h and the use of nondenaturing transfer buffers permits proteins to be blotted in the native state.

TL;DR: The stabler, more inert nature of Nycodenz endows it with a number of favorable properties as a gradient medium.

TL;DR: A sensitive fluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described and the determination of DNA in cells grown as a monolayer by the described method is simple and rapid.

TL;DR: Phosphofructokinase has been isolated in homogenous form from baker's yeast in aqueous biphasic systems containing Cibacron blue F3G-A-polyethylene glycol and the amount of contaminating proteases was reduced by 2 orders of magnitude.