Showing papers in "Biosensors and Bioelectronics in 2020"

TL;DR: Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance.

TL;DR: The COVID-19 mRT-LAMP-LFB assay is a promising tool for diagnosing SARS-CoV-2 infections in frontline public health field and clinical laboratories, especially from resource-poor regions.

TL;DR: This review provides general guidelines for both scientists in the biosensing research community and the biosensor industry to develop a highly sensitive and accurate point-of-care CO VID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

TL;DR: This review presents and evaluates two main types of diagnostics with FDA-EUA status for COVID-19: nucleic acid testing for detection of SARS-CoV-2 RNA, and serological assays for detection in patients with specific IgG and IgM patient antibodies, along with the necessary sample preparation for accurate diagnoses.

TL;DR: It is concluded that the detection methods targeting antibodies are not suitable for screening of early and asymptomatic cases since most patients had an antibody response at about 10 days after onset of symptoms, but antibody detection methods can be combined with quantitative real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) to significantly improve the sensitivity and specificity of diagnosis, and boost vaccine research.

TL;DR: This assay utilizes a custom CRISPR Cas12a/gRNA complex and a fluorescent probe to amplify target amplicons produced by standard RT-PCR or isothermal recombinase polymerase amplification (RPA) to allow sensitive detection at sites not equipped with real-time PCR systems required for qPCR diagnostics.

TL;DR: This review of the recent advances on enzymatic and non-enzymatic glucose sensors evolved in the last four years discusses the sensor fabrication methods, the materials and nanostructures involved, the detection principles and the performance of the sensors in whole blood, saliva, urine or interstitial fluids in detail.

TL;DR: This opinionated review critically discusses the state-of-the-art biosensing devices for COVID-19 testing and spot the urgent needs and highlight innovative diagnostic approaches for targeting various CO VID-19 related biomarkers.

TL;DR: A single-layered, ultra-soft, smart textile for all-around physiological parameters monitoring and healthcare during sleep is presented to pave a new and practical pathway for physiological monitoring during sleep.

TL;DR: An opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in human plasma diluted in 1 mL of buffer solution, demonstrating that this platform offers a promising point-of-care testing tool to complement standard serological assays and make Sars-Cov-2 quantitative diagnostics easier, cheaper, and faster.

TL;DR: This review covers the technical aspects of integrating CRISPR/Cas technology in miniaturized sensors for analysis on-site and discusses the challenges of point-of-careCRISPR sensing.

TL;DR: This review presents an overview of the recent developments in new intrinsically stretchable piezoelectric materials and rigid inorganic pieZoelectrics materials with novel stretchable structures for flexible and stretchable PiezoeLECTric sensors and energy harvesters.

TL;DR: A critical and objective overview of the recent progress in high-sensitivity LFA detection technologies, which involve the exploitation of the physical and chemical responses of transducing particles, are provided.

TL;DR: This review summarizes recent developments of enzyme-free electrochemical and optical glucose sensors, as well as their respective wearable and commercially available devices, capable of detecting glucose at physiological pH conditions without the need to pretreat the biological fluids.

TL;DR: Conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR) are described.

TL;DR: A simple, inexpensive, and ultrasensitive DNA probe based LFB with CRISPR/Cas and loop-mediated Isothermal Amplification (namely CIA), which can detect as low as a single copy cloned Pseudomonas aeruginosa acyltransferase gene and clinical samples without DNA extraction/purification or advanced apparatuses.

TL;DR: The development of a high throughput, all-solution phase, and isothermal detection system for African Swine Fever Virus, which shows that this system is very specific and can differentiate nucleic acid targets with closely matched sequences.

TL;DR: An overview of the literature on carbonblack-based electrochemical (bio)sensors is presented, highlighting current trends and possible challenges to this rapidly developing area, with a special focus on the fabrication of carbon black-based electrodes in the realisation of sensors and biosensors.

TL;DR: A convenient, effective, and robust method for massive production of flexible and wearable humidity sensor is proposed, using laser direct writing technology to produce laser-induced graphene interdigital electrode (LIG-IDE), which is a promising candidate for next-generation electronics for intelligent agriculture.

TL;DR: The electrochemical aptasensor-based technology is promising in the detection of heavy metals with advantages of high sensitivity, specificity, and accuracy, but more researches should be carried out before this technology can be used for on-site detection.

TL;DR: This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods and discusses different fabrication techniques, detection principles, and applications of various virus biosensors.

TL;DR: Based on the personalized SERS signal analysis, NSCLC patients can be distinguished from the healthy controls easily and the advantage of clearly individual quantification may help the doctor to discover the relationship of exosomal PD-L1 and the immnuotherapy responds in individual level.

TL;DR: The current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus are summarized.

TL;DR: An in-depth evaluation of the nanostructured electrochemical sensing system for antibiotics residues in diverse matrices and an outlook on the future concepts of this research field to help upgrade the sensing techniques for antibiotics is provided.

TL;DR: The capability of nanoscale FeS2 to serve as enzyme mimics is investigated for the first time and a reliable colorimetric method is developed for detection of H2O2 and glutathione by a simple mix-and-detect strategy.

TL;DR: The proposed homogeneous circle-to-circle amplification eliminates the need for additional monomerization and ligation steps after the first round of RCA, and combines two amplification rounds in a one-pot reaction.

TL;DR: The development of an electrochemical biosensor through the immobilization of bacteriophage in surface-modified bacterial cellulose (BC) matrix that showed high specificity for S. aureus, both in pure and mixed cultures of non-host bacteria, and effectively discriminated live S.aureus in a mixture of live/dead cells in food samples.

TL;DR: A detailed classification and comprehensive discussion of recent works that harness these CRISPR-based diagnostic tools from a new perspective are provided, and current challenges and future perspectives of CRISpr-based diagnostics are outlined.

TL;DR: A contamination-free visual detection method for SARS-CoV-2 with LAMP and CRISPR/Cas12a technology that had high specificity and could avoid cross-reactivity with other common viruses such as influenza virus is reported.

TL;DR: The most advanced progress made in the key phases for future wearable and implantable technology from biosensing, wearable biosensing to AI-biosensing is summarized and material innovation, biorecognition element, signal acquisition and transportation, data processing and intelligence decision system are the most important parts.