scispace - formally typeset
Search or ask a question

Showing papers in "Clinica Chimica Acta in 1987"



Journal ArticleDOI
TL;DR: Automated enzymatic methods for cholesterol, triglycerides and free glycerol determinations, as well as a dextran sulfate-Mg2+ procedure for separation of high density lipoproteins (HDL) with standardization are described.

516 citations



Journal ArticleDOI
TL;DR: The ratios between the concentration quotients of the three immunoglobulins and that of albumin increase in a nonlinear fashion with progressive impairments of the blood CSF barrier, i.e. the molecular size dependent discrimination of the protein transfer ('selectivity') decreases.

447 citations




Journal ArticleDOI
TL;DR: Free steroid assays were performed by applying the same procedure to equilibrium dialysates obtained after dialysing plasma against an equal volume of buffer, instead of measuring tracer distribution, and tended to follow the plasma free and salivary cortisol pattern, both within and between the study groups.

122 citations




Journal ArticleDOI
TL;DR: The results show a highly significant increase of Lp(a) concentrations in blacks as compared to whites, and confirm the notion that Lp (a) lipoprotein levels are race-dependent.

97 citations


Journal ArticleDOI
TL;DR: Bile pigments and jaundice is one of the literary work in this world in suitable to be reading material as discussed by the authors. But this book is not suitable for all people and it is very appropriate for people with great curiosity.

Journal ArticleDOI
TL;DR: Apolipoproteins A-I and A-II from human high-density lipoproteins (HDL) were isolated and quantified by fast-protein liquid chromatography using a Superose 12 column (gel filtration) followed by a Mono Q column (anion-exchanger).

Journal ArticleDOI
TL;DR: The microheterogeneity of alpha 1-acid glycoprotein (AGP) has been studied in the sera of 48 patients with rheumatoid arthritis and of 12 healthy individuals and the relative amounts of AGP-variants observed in the healthy donors were similar to those observed in patients with activity grade I, but differed significantly from patients with grades II, III and IV.

Journal ArticleDOI
TL;DR: A simple assay was developed which permits the accurate determination of low residual arylsulfatase A activities in cultured skin fibroblasts of infantile, juvenile and adult MLD patients and pseudodeficient individuals.

Journal ArticleDOI
TL;DR: A highly specific and sensitive radioimmunoassay (RIA) for alpha-human atrial natriuretic peptide (hANP[1-28]) in plasma was developed and high correlations were found between the values obtained with these four antisera.

Journal ArticleDOI
TL;DR: In the hyperthyroid group the concentration of total cholesterol and low density lipoprotein-cholesterol increased significantly upon treatment and the ratio between high density cholesterol and total cholesterol decreased in the patients receiving propranolol.

Journal ArticleDOI
TL;DR: The concentration of lipid peroxides in the plasma and synovial fluid of 65 arthritic patients was determined using a new ion-pairing reverse phase HPLC technique, giving further support to the concept of oxygen-free radicals playing an important role in the pathogenesis of chronic inflammatory disorders.

Journal ArticleDOI
TL;DR: A significant positive correlation exists between the guanidinosuccinic acid, creatinine and guanidine levels in serum and cerebrospinal fluid, which could contribute to the complex nervous system symptomatology and the hematological complications seen in renal insufficiency.

Journal ArticleDOI
TL;DR: Data are consistent with the concept that the uromucoid glycoprotein can exist in several different polymeric forms under different ionic conditions.

Journal ArticleDOI
TL;DR: Radioimmunoprecipitation experiments of serum showed that tritiated endotoxin of E. coli 0 111: B 4 was only found in the precipitate obtained with anti-Gc-globulin, antitransferrin, and polyvalent antiserum against human serum.

Journal ArticleDOI
TL;DR: In this article, specific assays for human intestinal and liver alkaline phosphatases were developed by use of isozyme specific monoclonal antibodies bound to paper discs, and the assays are fast, specific and convenient to use as demonstrated by determinations of alkaline enzymes in sera and tissues.

Journal ArticleDOI
TL;DR: Addition of dithioerythritol and diethylenetriaminepenta-acetic acid to cerebrospinal fluid prevents breakdown of tetrahydrobiopterin for 6 mth at -70 degrees C and for up to 5 h at 4 degrees C.


Journal ArticleDOI
TL;DR: It is concluded that the hydrophobic bile acid deoxycholate, which does not desaturate cholesterol in bile, promotes the biliary excretion of arachidonic acid, a factor in cholesterol gallstone disease.


Journal ArticleDOI
TL;DR: Abnormally low PG I levels and PG I/PG II ratios were associated mainly with advanced stages of the intestinal type of cancer and therefore, are not useful screening tests to identify early cases of gastric cancer.

Journal ArticleDOI
Anni Gries1, J. Nimpf1, Martina Nimpf1, Helmut Wurm1, Gerhard M. Kostner1 
TL;DR: Apo A of the bottom fraction after ultracentrifugation and of the supernatant after selective precipitation is not stainable with Sudan black and probably represents a protein which is not associated with lipids.

Journal ArticleDOI
TL;DR: Using isoelectric focusing and crossed immunoelectrophoresis on ready-made Immobiline Dry Plates, pH 5-6, the amount of human serum transferrin in subfractions is significant different in sera of patients with diseases like CA, RA, haemochromatosis and insera of pregnant women.

Journal ArticleDOI
TL;DR: Findings suggest that urinary macromolecules play a key role in preventing calcium oxalate kidney stone disease by inhibiting the formation of large crystal aggregates and thereby reducing the probability of particle retention in the kidney tubules.

Journal ArticleDOI
TL;DR: The quantitative DBA method can be used to analyse over 170 samples per day compared to about 70 per day by RIA, and is therefore a fast and inexpensive alternative to cotinine assays for the assessment of smoking status and cigarette smoke intake.