Showing papers in "Clinical Biochemistry in 2011"

TL;DR: The goal of this review was to update recent data concerning routine identification of microorganisms by MALDI-TOF in the clinical microbiology laboratory.

TL;DR: Serum NO(x) measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer, and was linear, precise, and accurate.

TL;DR: The use of liquid chromatography hyphenated to mass spectrometry (LC/MS) has increased over the recent years, providing complementary results to those obtained with other approaches, and the input of LC/MS is reviewed and comments.

TL;DR: The evolvement of high resolution MS techniques and the development of data-independent detection modes have opened new possibilities for applications of LC-(MS/MS) in systematic toxicological screening analysis in the so called general unknown setting.

TL;DR: LC-MS/MS overcomes many of the limitations of immunoassays, enhances diagnostic utility of the testing, and expands diagnostic capabilities in endocrinology.

TL;DR: Insufficient antioxidant enzymes and increased oxidative stress may attribute to the risk of declining semen quality and hence protective role for antioxidant enzymes against the oxidative damage cannot be ruled out.

TL;DR: This Review analyses the newest notions about pathophysiological mechanisms of oxidative stress and inflammation in patients with renal diseases, also considering the different strategies studied to counterbalance this high risk state.

TL;DR: A new biomarker for joint degenerative diseases was developed, which was derived from MMP-degraded type II collagen, and levels were significantly higher in those individuals with mild or severe OA than in those with no OA.

TL;DR: Increased plasma levels of MG, cytokines, and adhesion molecules are associated with DN and these markers may be useful in predicting the development of DN.

TL;DR: Novel high-performance liquid chromatography tandem mass spectrometry methods have successfully dealt with problems inherent in many immunoassays for thyroid hormones and afforded improved specificity and accuracy in thyroid hormone measurements.

TL;DR: In peritoneal dialysis patients, elevated Cu/Zn ratios are associated with malnutrition, increased oxidative stress, inflammation, and disrupted immune status.

TL;DR: Assays using dried blood samples for the diagnosis of inborn errors of metabolism and some newer strategies including impregnation of the filter paper and filter filtration will be discussed.

TL;DR: LC-MS has unique advantages in measuring various vitamin D metabolites and analogues due to its flexibility, sensitivity, and specificity and will be used for standardizing serum 25(OH)D assays using reference materials available from the National Institute of Standards and Technology.

TL;DR: The results suggest that serum M MP-9 level is a better marker than serum MMP-2 in predicting the breast cancer development and progression.

TL;DR: Visceral fat levels increased with increasing glucose intolerance and are associated with decreased levels of adiponectin and increased levels of hs-CRP, TNF-alpha, oxidized LDL, visfatin, HOMA-IR and IMT.

TL;DR: ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allerGEN molecules.

TL;DR: TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus, suggesting that Nigella sativa oil and thymoquinone may prevent oxidative injury in various models.

TL;DR: HE4 demonstrated comparable diagnostic performances to CA125, though each marker had its own strengths and weaknesses, and combining CA125 and HE4 might be more advantageous than either one alone.

TL;DR: Developing fully automated LC-MS/MS front-end modules or MS/MS-based analyzers which offer a degree of user-friendliness and robustness similar to current standard clinical chemistry analyzers seems feasible today.

TL;DR: The UCP-LF IL-10 assay is a user-friendly, rapid alternative forIL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.

TL;DR: A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to hepatitis C RNA was presented in this paper.

TL;DR: Further research is needed to inform the optimal hs-TnT threshold and change in this setting.

TL;DR: TCA cycle metabolites and fatty acid metabolism were modified in the HF patients, indicating altered energy metabolism.

TL;DR: Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.

TL;DR: It is found that serum testosterone, progesterone, glucocorticoids, insulin and insulin-like growth factors are increased in patients with acne vulgaris and serum estrogen levels are low in patients.

TL;DR: Liquid chromatography-tandem mass spectrometry can detect several disorders with a single injection, which is important in high throughput laboratories, and the clinical implications of applying this technology to NBS on a large scale in many jurisdictions across the world are discussed.

TL;DR: This study demonstrated that sRAGE was independently associated with death in ARDS patients and suggested active involvement of HMGB1-RAGE axis in poor prognosis of ARDS.

TL;DR: This review overviews current LC-MS/MS methods used for the quantification of biogenic amines and their metabolites and enables not only more specific analyte selection, but also automation of the complicated clean-up procedure.

TL;DR: IMA levels increase in overweight and obese subjects and its association with body mass index (BMI) in patients who are obese is evaluated.

TL;DR: Vitamin D status, age and gender are important for establishing reference intervals of bone markers in healthy children and all the bone marker levels, except total alkaline phosphatase, were significantly higher in puberty with vitamin D(3) >75nmol/L.