Showing papers in "Cryobiology in 2009"

TL;DR: The existing evidence regarding tumor cryo-immunology is reviewed and hypotheses regarding patient, tumor and technical factors that may influence the resultant immune response and warrant further investigation are put forward.

TL;DR: In vivo experiments with major organs, including wound healing, the putative immunological response following thawing, and the use of cryoadjunctive strategies to enhance cancer cell sensitivity to freezing, are described.

TL;DR: The lethality of slow warming is interpreted to be a consequence of it allowing time for the growth of small intracellular ice crystals by recrystallization, and the relative influences of warming rate and cooling rate on the survival of mouse oocytes subjected to a vitrification procedure.

TL;DR: This study investigated additional processing and cryobiological characteristics of DPSC, and determined that Me(2)SO at a concentration between 1 and 1.5M was the ideal cryopreservative of the three studied.

TL;DR: In biochemical assays, the additives did not show effectiveness on the elimination of malondialdehyde (MDA) formation and maintenance of GSH and GSH-Px activities, when compared to controls, and no significant differences were observed in non-return rates among groups.

TL;DR: Alpha-tocopherol, supplemented at 200 microM concentration in boar semen extender during cryopreservation had a positive effect on post-thawed sperm survivability and expression of pro-apoptotic genes was significantly higher and anti-apOptotic genes were significantly lower.

TL;DR: Adding cholesterol to spermatozoal membranes reduces the amount of osmotic stress endured by stallion spermatozosa during cryopreservation.

TL;DR: The highest concentrations of radicals were detected in the dried bacteria with highest survival for a given water activity, pointing towards a role of semi-stable ascorbyl radicals as a "dead end" for otherwise detrimental free radical reactions.

TL;DR: It was noted that there were wide individual variations both in sperm viability and in motility, and it was decided to evaluate the cryoprotective effect of glycerol/trehalose mixtures.

TL;DR: Viability of long preserved liver grafts can be augmented by transient hypothermic reconditioning using either machine perfusion or gaseous oxygen persufflation, both preventing initial mitochondrial dysfunction and subsequent tissue injury.

TL;DR: This data provides the first report of differential regulation of microRNA species in a freeze tolerant vertebrate and suggests a mechanism for rapid, yet reversible, gene silencing when animals transition into the frozen state.

TL;DR: It is possible to cryopreserve preantral follicles from pig ovarian tissue using Me2SO or EG, as well as other cryoprotectants such as ethylene glycol and propanediol.

TL;DR: Flow cytometry and morphology assessment showed that liposome treatment resulted in improved post-thaw membrane quality of RBCs following low temperature exposure.

TL;DR: Although both protocols impaired intercellular junctions, slow freezing represents a suitable method of GV stage cat oocytes banking since it more efficiently preserves the functional coupling with cumulus cells after thawing as well as nuclear and cytoplasmic competence.

TL;DR: In cells that were attached and spread on a substrate, IIF initiation sites were preferentially localized to the peripheral zone of the adherent cells, and non-uniformity in the spatial distribution of crystal centers contradicts predictions based on common theories of IIF, and provides evidence for a novel mechanism ofIIF in adherent cells.

TL;DR: Higher percentages of motile and viable sperm were maintained after thawing from bull sperm treated with CLC and pelargonate compared to all other treatments, whereas treatments with cyclodextrins pre-loaded with other cholesterol-like molecules did not.

TL;DR: It is suggested that CO-RM could be utilized as adjuvant therapeutics in UW solutions to limit the injury sustained by donor livers during cold storage prior to transplantation, as has been similarly proposed for the heart, and kidney.

TL;DR: Overall, this study indicates that the conventional cryopreservation of human heart valve allografts does not detrimentally affect their collagen and elastin structural integrity.

TL;DR: The accumulation of AFPs in extracellular spaces caused by seasonal cold acclimation during winter indicates that these proteins may play a role in the acquisition of freezing tolerance of needle cells in coniferous species.

TL;DR: The results of this experiment provide accurate permeation kinetics of four commonly used CPAs in porcine articular cartilage, which will be useful for developing effective vitrification protocols for cryopreservation of AC.

TL;DR: The full sequences of ice-binding proteins from shiitake and another mushroom, the cold-adapted Flammulina populicola (enoki mushroom), are reported and it is shown that the recombinant proteins have ice- binding activity.

TL;DR: Results indicated that, when the partially glassy and partially frozen state was intentionally allowed, the addition of PVA effectively inhibited not only ice nucleation but also ice growth in the vitrification solution.

TL;DR: The modeling predicts that IIF should not occur in yeast cooled at <20 degrees C/min and it should occur with near certainty in cells cooled at >or=30 degreesC/min, and experiments with differential scanning calorimetry confirmed these predictions closely.

TL;DR: It is demonstrated that delivery of AFPs into zebrafish embryos by incubation in media containing protein at early stages is a simple and harmless method that increases cryoprotection of the cellular compartment.

TL;DR: On the basis of the CPAs toxicity, PG and EG, in combination with PVP, seem to be most suitable for the cryopreservation of P. lividus eggs and embryos.

TL;DR: These experiments tested the potential benefit of incorporating a dehydration step prior to standard cryopreservation procedures for large equine embryos and found no advantage in incorporating a 2min dehydration step into the cryop Reservation protocol for large Equine embryos.

TL;DR: The results of the present study suggest the reconsideration of the ROP-technique for the preservation of predamaged donor grafts especially of NHBD and further studies, comparing MP and ROP upon long term preservation are strongly encouraged.

TL;DR: A novel cryoprobe for skin cryosurgery utilizing the Peltier effect is developed that makes it possible to control heat transfer to skin surface precisely using a proportional-integral-derivative (PID) controller, and because it uses the P Beltier effect, the Cryoprobe does not need to move during the operation.

TL;DR: Dimethyl sulfoxide (Me(2)SO) is the best cryoprotectant for European eel sperm, but increases the medium osmolality, inducing the activation of spermatozoa motility, which can be prevented using high NaHCO(3) concentrations, improving the cryopreservation process.

TL;DR: It is concluded that the freeze-thawing procedure spontaneously activated spermatozoa motility in common carp, however, this activation did not negatively affect the fertility of frozen-thawed sperm.