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Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes ☆

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TLDR
Alpha-tocopherol, supplemented at 200 microM concentration in boar semen extender during cryopreservation had a positive effect on post-thawed sperm survivability and expression of pro-apoptotic genes was significantly higher and anti-apOptotic genes were significantly lower.
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This article is published in Cryobiology.The article was published on 2009-04-01. It has received 135 citations till now. The article focuses on the topics: Sperm motility & Sperm.

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Cryopreservation of fish sperm: applications and perspectives

TL;DR: Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines but needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury.
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Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs.

TL;DR: The present work summarizes the principles of cryoinjury and the relevance of permeating and nonpermeating cryoprotective agents and speculates with new research horizons on the preservation of boar sperm, such as vitrification and freeze-drying.
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Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches.

TL;DR: Current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage are discussed, as well as evaluating the probable effects of sperm freezing on offspring health.
Journal ArticleDOI

The role of antioxidants in sperm freezing: a review

TL;DR: This review will clarify results of recent applications of various antioxidants used in numerous research efforts to improve cryopreservation of spermatozoa to increase the understanding of the roles of these antioxidants concerning mechanisms which enhance resistance to cryodamage of spermutozoa.
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Factors enhancing fish sperm quality and emerging tools for sperm analysis

TL;DR: A comprehensive overview of the current methods used in research to assess sperm quality is given, in new trends on sperm analysis, and in the use of these tools for the identification of factors enhancing gamete quality.
References
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Journal ArticleDOI

Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death

TL;DR: Overexpressed Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line and counters the death repressor activity of B cl-2, suggesting a model in which the ratio of Bcl-2 to Bax determines survival or death following an apoptotic stimulus.
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BCL-2 FAMILY: Regulators of Cell Death

TL;DR: The BCL-2 family reveals evidence for a cell-autonomous coordination between the opposing pathways of proliferation and cell death, and acts as a checkpoint upstream of CASPASES and mitochondrial dysfunction.
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Protein damage and degradation by oxygen radicals. I. general aspects.

TL;DR: The results indicate a general sensitivity of proteins to oxygen radicals and indicate that proteins which have been denatured by .OH can be recognized and degraded rapidly and selectively by intracellular proteolytic systems.
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Role of the human heat shock protein hsp70 in protection against stress-induced apoptosis.

TL;DR: The inhibition of SAPK/JNK signaling and apoptotic protease effector steps by hsp70 likely contributes to the resistance to stress-induced apoptosis seen in transiently induced thermotolerance.
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