Determination of DNA base composition by reversed-phase high-performance liquid chromatography
Jin Tamaoka,Kazuo Komagata +1 more
TLDR
The system described here gives a direct and precise method for determining DNA base composition by reversed-phase high-performance liquid chromatography (HPLC).Abstract:
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromatography needed 2 μg of hydrolysed nucleosides and took only 8 min. The relative standard error of nucleoside analysis was less than 1%. The system described here gives a direct and precise method for determining DNA base composition.read more
Citations
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Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography
TL;DR: High-performance liquid chromatography is a promising alternative for determining the G+C content of bacterial deoxyribonucleic acid (DNA) and may also be more accurate than indirect methods, such as the buoyant density and thermal denaturation methods.
Journal ArticleDOI
Rapid extraction of bacterial genomic DNA with guanidium thiocyanate
TL;DR: The method, which was applicable to both Gram‐positive and Gram‐negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments.
Journal ArticleDOI
A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature.
TL;DR: A novel fluorimetric method to estimate the G+C mol% content in microorganisms using double-stranded DNA and its thermal denaturalization was followed by measuring a decrease in fluorescence using a real-time PCR thermocycler.
Journal ArticleDOI
Notes on the characterization of prokaryote strains for taxonomic purposes.
TL;DR: The purpose of the present article is to outline the key elements in the way that prokaryotes are characterized, with a view to providing an overview of some of the pitfalls commonly encountered in taxonomic papers.
Journal ArticleDOI
Proposal of Burkholderia gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.
Eiko Yabuuchi,Yoshimasa Kosako,Hiroshi Oyaizu,Ikuya Yano,Hisako Hotta,Yasuhiro Hashimoto,Takayuki Ezaki,Michio Arakawa +7 more
TL;DR: A new genus Burkholderia is proposed for the RNA homology group II of genus Pseudomonas and seven species in this group were transfered to the new genus.
References
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Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J. Marmur,Paul Doty +1 more
TL;DR: The previously discovered linear relation between the base composition of DNA, expressed in percentage of guanine plus cytosine bases, and the denaturation temperature, T m, has been further investigated and it appears that the measurement of the T m is a satisfactory means of determining base composition in DNA.
Journal ArticleDOI
Preparation of transforming deoxyribonucleic acid by phenol treatment
Hiuga Saito,Kin-ichiro Miura +1 more
TL;DR: The final DNA preparation has high transforming activity, especially for the joint transformation of linked markers, as well as high purity and low sedimentation coefficients.
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Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Journal ArticleDOI
A rapid method for the base ratio determination of bacterial DNA
TL;DR: A rapid, simple, and inexpensive procedure is described for the isolation and base analysis of bacterial deoxyribonucleic acids (DNA) in samples of 0.2 mg.
Journal ArticleDOI
Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
TL;DR: In this paper, the base composition of deoxyribonucleic acid (DNA) was calculated by regression and correlation analysis and treated statistically by using only sets of data on DNA determined with the same strains.