Showing papers in "Journal of Biotechnology in 1994"

TL;DR: Predictive reports suggest that 10% of the recombinant proteins, corresponding to a 100 million dollars annual market, will be prepared from the milk of transgenic animals by the end of the century, and progress to improving the efficiency of this process inclines to be reasonably optimistic.

TL;DR: Dielectrophoresis, the movement of particles in non-uniform AC electric fields, was used to rapidly separate viable and non-viable yeast cells with good efficiency and cell viability was not affected by the separation procedure.

TL;DR: Cold-adapted microorganisms have a considerable potential in biotechnological application (waste treatment at ambient temperatures, enzymology, food industry, medicine).

TL;DR: The increased expression of the Bm86 antigen from the cattle tick Boophilus microplus in the methylotrophic yeast Pichia pastoris permits the obtainment in a high yield of the tick Bm 86 antigen, in a glycosylated and particulated form.

TL;DR: An improved technique of hybrid modelling biochemical production processes is described, composed of a set of dynamical differential equations, an artificial neural network and a fuzzy expert system, demonstrating the applicability of a hybrid model for state estimation, prediction, feed rate optimization, and process control.

TL;DR: Ferulic acid metabolism was studied in cultures of the white-rot fungus Pycnoporus cinnabarinus I-937, where the enzyme countered the vanillin formation by promoting the polymerization of ferulic acid into lignin-like polymers.

TL;DR: Plasmids containing the Alcaligenes eutrophus poly(3-hydroxybutyric acid) (PHB) biosynthetic genes were constructed for the production of PHB in Escherichia coli and plasmid stability was investigated by repeated subculturing without antibiotic pressure, suggesting that high gene dosage was required for the synthesis and accumulation to a high concentration in E. coli.

TL;DR: Trichoderma reesei can be generally considered not only a safe production organism of its natural enzymes but also a safe host for other harmless gene products.

TL;DR: Non-chromatographic affinity technologies aim at high throughput and seek to circumvent problems associated with diffusion limitations experienced with most chromatographic packings.

TL;DR: In this article, coniferyl aldehyde was converted to a wine-red lignin polymer with peroxidase/H2O2 and the color of the lignins was very similar to that of lignified tissues of transgenic tobacco plants with an antisense gene of cinnamyl alcohol dehydrogenase (CAD).

TL;DR: A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen and carbon dioxide in bioreactors is presented and accuracy, resolution and reproducibility fulfill the requirements for use in biotechnological applications.

TL;DR: A review of the current research on the use of biotechnological methods to remove colour from pulp and paper mill wastewater is presented in this article, where the progress made and the further scope are also discussed.

TL;DR: The results indicate that fed-batch cultivation strategies are well suited to produce recombinant gene products and passed a pronounced maximum value in dependence of the set growth rate.

TL;DR: Optimal conditions for submerged cultivation of the white rot fungus Panus tigrinus are developed, which increase the yield of extracellular ligninolytic enzymes and 3-methylbenzyl alcohol as inducer.

TL;DR: It was shown that the cell wall-bound and the liberated lactonic sophorose lipid were identical and had a hydroxy Δ 8 -hexadecenoic acid backbone and the physiological significance of this lipid to act as extracellular carbon storage material was discussed.

TL;DR: In all cases, alpha-chymotrypsin exhibited non-first-order deactivation kinetics, corresponding to a two-step unimolecular mechanism, where the main protective effect of polyols was observed in the first-step of the deactivation profile.

TL;DR: It has been suggested that D-amino acid production from DL-5-substituted hydantoin involves the action of a series of enzymes involved in pyrimidine degradation, namely amide-ring opening enzyme, dihydropyrimidinase, and N-carbamoylamide hydrolyzing enzyme, beta-ureidopropionase.

TL;DR: In this article, commercial xylanases, Novozyme 473, VAI Xylanase and Cartazyme HS-10 were evaluated for prebleaching of eucalyptus kraft pulp.

TL;DR: It was found that the peptone medium with xylan (2.0%) is more appropriate to achieve high productivity of xylanase in bioreactor system, due to rheological problem under higher biomass formation with accompanying viscosity and consequent oxygen transfer efficiency.

TL;DR: Theoretical analysis and preliminary computer simulations indicate that about 100-200 sequence specific hybridisations of octanucleotides to about 100,000 PCR products of 1000-1500 base-pairs length will generate sufficient information for classifying the clones into groups of identical or related genes and to identify a large number of previously uncharacterized cDNA clones.

TL;DR: The requirements for a database that collects information about regulatory DNA sequences are discussed and the structure and contents of such a database (TRANSFAC) are described to facilitate the recognition of regulatory genomic sequence information and the assignment of the corresponding regulators.

TL;DR: Anti-tumor activity analyses showed that r-AD inhibited the growth of two mouse cell lines, hepatoma MH134 and fibrosarcoma Meth A, strongly in vitro at concentrations in excess of 10 ng ml-1.

TL;DR: It is proposed that the mechanism of dipeptide utilisation involves extracellular hydrolysis by this peptidase following its release into the culture medium.

TL;DR: The metabolic activity was found in cells immobilized in calcium alginate beads older than 5 years and having been stored for the entire time in 4°C water as nongrowing and starveling immobilized culture.

TL;DR: The oxidoreductase accepted as substrate aliphatic and aromatic ketones, keto esters (esters of keto carboxylic acids) and halogenated carbonyl compounds and reduced them to the corresponding hydroxyl compounds with (S)-configuration with more than 98% enantiomeric excess.

TL;DR: A reproducible procedure for transformation of protoplasts and regeneration of transgenic plants for an improved Indica rice cultivar IR43 is established and stable integration of the transgene in the genome of these plants was confirmed.

TL;DR: It is shown that maximal respiratory capacity is not permanently available and that cells develop some (not investigated) mechanism to cope with the suppression which is reflected by a forgetting function that allows the upper limit to approach the maximal value.

TL;DR: Analysis of one such transformant provided evidence that the overexpression of the glaA gene is limited by the amount of trans-acting regulatory protein(s) available, and suggested that newly introduced gene copies were heavily rearranged, which partly explains why GLA production was not increased.

TL;DR: In this article, a site-directed mutagenesis of the CGTase gene of Bacillus ohbensis replacing Tyr at position 188 by 19 other amino acids was described.

TL;DR: Bacteria were enriched from soil samples with succinate as a carbon source and racemic naproxen nitrile [2-(6-methoxy-2-naphthyl)propionitrile] as sole source of nitrogen to identify Rhodococci which were identified as rhodococci.