Showing papers in "Journal of Clinical Virology in 2022"

TL;DR: In this paper , the authors compared clinical data with longitudinal qPCR results from lesion swabs, oropharyngeal swabs and blood in a well characterized patient cohort.

TL;DR: In this article , the authors evaluated SARS-CoV-2 antibody response over 5-430 days on 828 samples across COVID-19 severity levels, for total antibody (TAb), IgG, IgA, IgM, neutralizing antibody (NAb), antibody avidity, and for receptor-binding-domain (RBD), spike (S), or nucleoprotein (N).

TL;DR: In this paper , a novel interferon-γ release assay (IGRA) was proposed to detect SARS-CoV-2 specific T cell response to COVID-19 as well as COVID19 vaccination, with sensitivity getting lower over time.

TL;DR: In this paper , the capacity of Abbott molecular, antigen, and serologic assays to detect circulating SARS-CoV-2 variants, including all current variants of concern (VOC), was evaluated.

TL;DR: The authors in this paper studied the genetic characteristics of respiratory syncytial virus (RSV) circulating strains in paediatric patients in Buenos Aires, Argentina before and during the COVID-19 pandemic.

TL;DR: In this article , the authors evaluated the performance of RT-PCR on saliva swabs and antigen testing on mid-turbinate MT samples relative to RT-pcR on MT swabs.

TL;DR: In this article , the authors showed that BA.5 subvariants of SARS-CoV-2 (B.12.1, BA.4 and BA.2) are spreading globally and demonstrate higher transmissibility and immune escape.

TL;DR: In this paper , the envelope gene was used as internal control to detect SARS-CoV-2 Spike indel_211 and indel-214 with a 95% lower bound of detection of 1.3 copies/µLuate.

TL;DR: In this article , the authors compared an in-house ELISA (KWTRP) ELISA with WANTAI, an ELISA which has been approved for widespread use by the WHO.

TL;DR: In this article , the authors collected serial samples from 23 hospitalized COVID-19 patients with mild symptoms and measured the kinetics of SARS-CoV-2 viral load in respiratory samples and markers of inflammation in serum samples.

TL;DR: In this paper , a double-mismatch allele-specific RT-PCR (DMAS-RT-PCL) was developed to discriminate reliably between the Delta variant and other variants (Alpha and Beta), and wild-type SARS-CoV-2 (B.1.617.2).

TL;DR: For example, the Roche cobas® 6800/8800 analyzer as discussed by the authors was used for quantification of circulating hepatitis B RNA (cirB-RNA), which is a promising biomarker for monitoring antiviral treatment.

TL;DR: In this article , the authors describe the first month of MPXV clinical laboratory diagnosis, including assay utilization and positivity, and detail the Johns Hopkins analytical validation of a qualitative orthopoxvirus real-time PCR and characterize its analytical performance for testing.

TL;DR: In this paper , Meso Scale Diagnostics' V-PLEX Coronavirus Panel 2 allows for highly sensitive and specific detection of anti-coronaviral IgG, and is concordant with other serological assays for detection of antibodies against SARS-CoV-2 and the endemic human coronaviruses.

TL;DR: In this article , the NVX-CoV2373-vaccine has been licensed, although knowledge on vaccine-induced humoral and cellular immunity towards the parental strain and variants of concern (VOCs) in comparison to mRNA-regimens is limited.

TL;DR: In this paper , the BinaxNOW COVID-19 Ag Card rapid antigen assay (Abbott; Chicago, IL, USA) compared to the reference standard of PCR testing was evaluated.

TL;DR: Torque teno virus (TTV) DNA load in plasma directly associates with the net state of immunosuppression and inflammation in different clinical settings, including transplantation and chronic inflammatory diseases as discussed by the authors .

TL;DR: In this paper , the authors used three duplex RT-ddPCR reactions targeting four amino acids to identify variants including Omicron in clinical specimens to enable case tracking and/or selection of appropriate clinical treatment.

TL;DR: In this paper , the authors developed a RT-PCR assay for the specific detection of the SARS-CoV-2 Omicron Variant of Concern (VOC) as a rapid alternative to sequencing.

TL;DR: In this article , the authors measured and evaluated the reduced sensitivity of Lambda variant to the neutralization by plasma polyclonal antibodies elicited by the natural SARS-CoV-2 infection and inactivated vaccine.

TL;DR: In this article , the authors analyzed whether viral load in the upper respiratory tract is correlated with transmission and disease course and how this information can be used in practice and found that higher viral loads were significantly correlated with older age and with more (severe) COVID-19 related symptoms.

TL;DR: In this article , the temporal trends in SARS-CoV-2 Cycle threshold (Ct) values from clinical testing were analyzed to determine if temporal trends were predictive of future cases, which can facilitate improved hospital-level surge planning.

TL;DR: In this paper , the authors investigated the pathogenic agent responsible for the outbreak and the circulation of arboviruses in the region of the Expedito Ribeiro Settlement, Pará State, Brazil.

TL;DR: In this article , the authors investigated whether a positive test is associated with a high anti-viral IgG, specific viral variant, recovery of infectious virus, or symptomatic infection during an early phase after vaccination rollout.

TL;DR: In this paper , the authors performed a retrospective observational cohort study at a large Northern California healthcare system of infection-naïve individuals fully vaccinated against SARS-CoV-2 (mRNA-1273, BNT162b2, or Ad26.COV2).

TL;DR: In this article , the results of the Plaque Reduction Neutralization Test (PRNT) were used to compare the fold reduction in antibody titers against the variants of concerns of SARS-CoV-2 between the primary and booster dose vaccine cohorts of inactivated BBIBP-CorV vaccine.

TL;DR: In this article , the authors evaluated the accuracy of tests for IgM and IgG antibodies for SARS-CoV-2 infection confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) and utility as complementary data for immunosurveillance.

TL;DR: In this paper , the authors analyzed trends of SARS-CoV-2 anti-nucleocapsid IgG throughout the four rounds of the seroepidemiologic study ENE-COVID, and compared the fourth-round results of two immunoassays detecting anti-RNIs and anti-RBD IgG.

TL;DR: Lateral flow rapid antigen tests showed a higher rate of false negatives in general compared with automated point-of-care tests and laboratory ELISA/immunoassays and support ongoing assay evaluations for regulatory approval or post-market surveillance.

TL;DR: In this paper , immunogenicity data was evaluated using LIAISON® SARS-CoV-2 TrimericS IgG assay (DiaSorin S.p.A).