Showing papers in "Journal of Lipid Research in 1979"

TL;DR: The capacity of lipoprotein production and hyperlipemia development increases during chronic alcohol consumption, probably as a result of the concomitant hypertrophy of the endoplasmic reticulum and Golgi apparatus, however, this compensation is relatively inefficient in ridding the liver of fat.

TL;DR: A rapid sensitive, and reproducible procedure is described for the analysis of alpha-tocopherol in blood cells and plasma using high-performance liquid chromatography and fluorometric detection.

TL;DR: Four subfractions of plasma VLDL characterized by decreasing Sf value and LDL were isolated by density gradient preparative ultracentrifugation from normotriglyceridemic and hypertriglyceridemia subjects in the fasting state and after a fatty meal to show that the hyperlipidemia of type IV subjects was accounted for by an increase in total numbers of VLDl and a shift in the distribution of V LDL towards particles of larger diameter.

TL;DR: The observation of a smectic liquid crystalline phase for hydrated cholesterol correlates with its high surface activity and helps to explain its ability to exist in high concentrations in biological membranes.

TL;DR: It is concluded that the specific LDL receptor mechanism operates in vivo and probably accounts for 33% and 16% of overall LDL catabolism in normal and heterozygous familial hypercholesterolemic subjects, respectively.

TL;DR: These studies document the large and highly variable errors inherent in estimating rates of sterol synthesis in extrahepatic tissues using 14C-labeled substrates under in vitro conditions.

TL;DR: Fatty acid modifications were produced in the commercially available IMR-90 strain of human lung fibroblasts, suggesting that the ability to tolerate considerable differences in fatty acid composition is not a special property of the skin fibroblast line that was isolated locally.

TL;DR: The feeding of a high-fat diet to adult rats was shown to increase the incorporation of [3H]thymidine into DNA of the adipocyte and stromal fractions, and rats labeled when young and later placed on a high -fat diet showed a decrease in DNA specific activity in both adipocytes and stroma, confirming that cellular proliferation had occurred in both fractions.

TL;DR: Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances, allowing identification and quantitative analysis of phospholips in a mixture, and the chemical shift depends strongly on pH.

TL;DR: A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates.

TL;DR: Substrate competition experiments showed that cholic acid 7 alpha-dehydroxylation was reduced by increasing concentrations of chendeoxycholic acid; however, chenodeoxycholics acid 7alpha- dehydroxyation activity was unaffected by increasing concentration of cholic Acid.

TL;DR: A simple and rapid mixed-phase method for the quantitative assay of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase and a procedure for the efficient reactivation of Mg-ATP-inactivated microsomal HMG-CoA by potato acid phosphatase are described in this paper.

TL;DR: It was concluded that diosgenin interferes with the absorption of cholesterol of both exogenous and endogenous origin; such interference is accompanied by derepressed rates of hepatic and intestinal cholesterol synthesis.

TL;DR: The structural properties and functions of the plasma high density lipoproteins (HDL) have recently become the center of attention, largely because of their possible participation in the control of both lipid metabolism and the pathogenesis of atherosclerosis.

TL;DR: Compared with normal brain, all tumors had greater proportions of the structurally less complex gangliosides and smaller proportions ofThe more complex gangLiosides was most marked in the rapidly growing tumors while the better differentiated astrocytomas contained the greatest proportions of complexganglioside.

TL;DR: Excess adiposity develops in the fa/fa rat during the first week of life, before hypertriglyceridemia and hyperphagia, and raises the question of whether this adiposity results from a defect in energy expenditure or an abnormality of fat cell storage capacity, or both.

TL;DR: The present state of knowledge in this field includes the chemistry, physical chemistry, and physiological chemistry of pancreatic colipase and its interactions.

TL;DR: High density lipoproteins contains subpopulations that differ in the A-I/A-II molar ratio and cholesterol/(A-I + A-II) ratios when isolated from men or women are examined.

TL;DR: The differential hydrophobic interaction observed between the ligand of the stationary phase and different alkyl chains of the sphingomyelin species illustrates that reversed-phase HPLC technique can be conveniently used to study the extent of relative hydrophobicity of different types of alkyal chains.

TL;DR: In comparison to untransformed 3T3 whole cells, SV3T3 cells showed an unchanged content of triacylglycerols, free fatty acids, and glycerylether diesters but a lower concentration of total phospholipids, while no significant difference was found in the phosphate composition.

TL;DR: It was shown that biliary cholesterol output could be driven by bile acid infusion to a similar extent in rats in which the rate of hepatic cholesterogenesis had been varied over a 26-fold range.

TL;DR: A method for solubilizing HMG-CoA reductase is described that reproducibly yielded approximately 190% of the activity assayed in rat liver microsomes, and the kinetics were determined.

TL;DR: The sodium dodecyl sulfate-resistant lipolytic activity has the characteristics of LPL as judged by a) its activation by serum and by apolipoprotein C-II; b) its inactivation by 0.75 M NaCl; and c) itsInactivation by a specific antiserum.

TL;DR: A high-yield synthesis of saturated, unsaturated, and short chain phosphatidylcholines from sn-glycero-3-phosphocholine from 4-pyrrolidinopyridine as a catalyst and moderate amounts of fatty acid anhydride is described.

TL;DR: The data strongly suggest that the blunted response of glucose metabolism to insulin in adipocytes of high-fat-fed rats is a result of a decreased intracellular capacity to utilize glucose for lipogenesis.

TL;DR: It is suggested that during in vitro lipolysis of VLDL, surface constituents leave the lipoprotein concomitantly with the hydrolysis of core triglycerides, independent of the presence of an acceptor lipop Protein and may occur in the form of a surface-fragment particle.

TL;DR: The results suggest that the observed RPH activity is relatively specific for the hydrolysis of retinyl palmitate, and may therefore be significantly involved in hepatic retinyl ester metabolism.

TL;DR: The mevalonate content of the plasma from the blood of the vena cava inferior of male rats varied between 81 and 502 pmol/ml and is positively related to the levels of liver 3-hydroxy-3-methylgultaryl-CoA reductase, suggesting that the liver is probably the main source of meValonate circulating in blood.

TL;DR: It is concluded that future studies of cholesterol metabolism utilizing human mononuclear cells must take into account this large variation in the percentage of monocytes and their disproportionately greater activity during short-term incubations in media that induce sterol synthesis.

TL;DR: These studies suggest that the detergent may achieve its hyperlipidemic effct by disrupting HDL and thus removing the A-I and C-II proteins from a normal activating environment compirsing VLDL, HDL, and the enzymes.