Showing papers in "Laboratory Investigation in 1990"

TL;DR: It is concluded that myofibroblasts develop gradually from granulation tissue fibroblast and temporarily express a marker of smooth muscle differentiation, which may be relevant for the understanding of the mechanisms of normal and pathologic wound healing.

TL;DR: The hypothesis most consistent with all available data suggests that 02 reduces a cellular source of ferric to ferrous iron, and the latter then reacts with H202 to produce a more potent oxidizing species, like the *OH or an equivalently reactive species.

TL;DR: It is reported that rings of rat aorta embedded in gels of fibrin or collagen and cultured in MCDB 131, an optimized growth medium for microvascular endothelial cells, generate branching microvessels in the absence of serum or other soluble protein supplements.

TL;DR: In virtually every facet of the mast cell’s natural history, from its differentiation and maturation to its biochemistry and function, recent work has revealed considerably more complexity than had previously been suspected to exist.

TL;DR: Lower risk of breast cancer has been reported in women that have had an early full-term pregnancy and among all of these factors, the development of Breast cancer is influenced by the reproductive history of the individual.

TL;DR: After the fetal and postnatal growth of the liver, hepatocytes no longer proliferate actively, but it is a common observation that they divide in response to hepatic cell death or loss of liver tissue.

TL;DR: The distribution of basic fibroblast growth factor was studied immunohistochemically in fresh frozen sections of normal human tissues and the presence of bFGF in the extracellular compartment of a diverse variety of organs may play a role in angiogenesis.

TL;DR: The results are in accord with specific homing and attachment of lymphocytes to the CNS vasculature being early features of the disease process in EAE and with some CNS vessels acquiring properties of lymph node elements.

TL;DR: It is concluded that in this model, MBP-immune lymphocytes act exclusively from a perivascular location to orchestrate the influx of inflammatory cells that are predominantly of recipient derivation.

TL;DR: A linear relationship was found between cell duplication activity and the amount of silver-stained NOR proteins independently of the type of cancer cells (r = 0.94, p less than 0.001), which could be used as a simple estimate of cancer cell proliferation rate.

TL;DR: Improved explanation method was developed which takes advantage of the tenuous attachment of OSE to underlying tissues: the surface epithelium was scraped off the ovarian surface with a rubber scraper, generating epithelial fragments which produced monolayers in culture, with little contamination by other cell types.

TL;DR: New electron microscopic immunocytochemical findings in the laboratory suggest that the origin of alkaline phosphatase-positive bone matrix vesicles is polarized to the mineral-facing side of osteoblasts and may be concentrated near the intercellular junctions of human embryonic osteoblasting.

TL;DR: Findings suggest that CD34 is an endothelial marker for EAM present during angiogenesis, and is preferentially found on a small subset of cells with the morphologic appearance of migrating cells.

TL;DR: The amount of tenascin was moderately enhanced in certain physiologic conditions (fetal growth, gestation), as well as hyperplasias, dysplasia (fibrocystic disease) and benign tumors, whereas it was markedly enhanced in intraductal and infiltrating carcinomas.

TL;DR: Immunocytochemical techniques used to identify and localize heparan sulfate proteoglycans (HSPGs) in human cases of Gerstmann-Straussler syndrome and Creutzfeldt-Jakob disease, as well as in experimental scrapie of hamsters demonstrate that both the protein core and the GAG chains of HSPGs are immunolocalized to the prion protein amyloid plaques in each of these diseases.

TL;DR: The spectrum of ubiquitin-immunoreactive structures in normal brains are demonstrated and progressive axonal dystrophy may be a more common age-related pathologic alteration of the brain than formerly recognized.

TL;DR: The results support the conclusion that the endothelioma cell line maintains characteristic endothelial cell properties and behaves in vitro in a manner similar to microvascular endothelial cells.

TL;DR: The authors found similar fibronectin profiles in suction blister fluid (2 normal volunteers) and mastectomy fluid (5 patients) and there was primarily an intact 250 kilodalton fibronECTin subunit and in addition some higher molecular mass fibronsectin-containing complexes.

TL;DR: The hypothesis that tumor promoters stimulate cell proliferation of initiated cells by inhibiting gap junctional intercellular communication in the initiated cells is proposed.

TL;DR: It is believed that, in the main, IgAN is an immune complex disease resulting from a poorly controlled mucosal immune response to environmental antigens to which the host is chronically subject.

TL;DR: The temporal and cellular distribution of transforming growth factor (TGF)-beta 1 and procollagen alpha 1(I) transcripts were examined during regeneration and early fibrosis of rat liver using in situ hybridization and Northern blot analyses to suggest that TGF-beta 1 derived from inflammatory cells may have enhanced the expression of the Procollagenalpha 1 (I) gene by paracrine mechanisms.

TL;DR: It is shown that TGF-beta 1 was auto-stimulating in wounds, accounting, at least in part, for the persistent effects of single doses of this multipotential cytokine.

TL;DR: The findings indicate that mast cells occur in close proximity to neural processes in sites where these structures are abundant, but that anatomical associations as close as those between mast cells and neural processes can also occur between such structures and other bone marrow-derived cells (eosinophils) or lymphoid cells (plasma cells) resident in the small intestinal mucosa.

TL;DR: Two human nasopharyngeal carcinoma (NPC) cell lines have been established and the karyotypic analysis showed multiple chromosomal abnormality, and Ultrastructural studies from those two cell line showed two types of morphology: the dark and light cells.

TL;DR: A hypothesis that high molecular weight glycoproteins produced by colorectal carcinoma tissues are heterogeneous with regard to their carbohydrate chains and their antigenic structures may change during tumor progression is supported.

TL;DR: An improved method of embedding a large number of tissue samples in a single, normal-sized paraffin block permits rapid and inexpensive screening of new histologic reagents, and facilitates intra- and interlaboratory quality control.

TL;DR: In interstitial fibrosis, as well as several other forms of lung injury, there is an apparent increase in smooth muscle cells organized into bundles within areas of the lung where these cells normally are present but difficult to detect, such as the alveolar duct.

TL;DR: The data suggest that the vascular system is a source of APP and that the processing of APP into insoluble fibrils in AD and HCHWA-D may take place in situ.

TL;DR: Evidence of regeneration was sought in atrophic kidneys after surgical reversal of renal artery stenosis and, in other animals, after unilateral nephrectomy of the contralateral kidney, and showed that regeneration, involving both hypertrophy and hyperplasia, was stimulated only by removal of the hypertrophied contralsateral kidney and occurred whether or not stenosis of the renal artery was reversed.