Showing papers in "Metabolic Engineering in 2022"

TL;DR: In this article , the authors summarized well-known monomer degradation pathways and computed their theoretical yields for industrially interesting products, and calculated replacement scenarios of existing fossil-based synthesis routes for the same products.

TL;DR: In this article , the authors provide an overview of the systems-level understanding of metabolism, key metabolic pathways, scale-up opportunities and commercial successes, and the most recent technological advances in strain and process engineering.

TL;DR: In this paper , a growth phase-dependent autonomous bifunctional genetic switch (GABS) was proposed to dynamically switch from cell growth to production for high-level GABA production from low-value glycerol in Corynebacterium glutamicum.

TL;DR: The natural pathway atlas as discussed by the authors provides a guiding star for metabolic engineers to breed designed cell factories and efficiently upgrade this global waste stream, which offers a sustainable product portfolio from lignin, comprising bulk and specialty chemicals, biomaterials, and fuels.

TL;DR: In this paper , three emerging engineering strategies are discussed: designing dynamic control of metabolic pathways, engineering strains of halophilic (salt-tolerant) microbes, and developing microbial consortia for conversion.

TL;DR: In this article , the authors explore the metabolic bottlenecks of the microbial communities adopting latest advances of profiling and 13C tracer-based analysis using state-of-the-art analytical platforms (GC, GC-MS, LC-MS and NMR).

TL;DR: In this paper , strategies for coping with the challenges are summarized in terms of enzymes, pathways, metabolic networks, host cells, and combined with protein structure prediction, de novo protein design, artificial intelligence (AI), biocatalytic retrosynthesis, and intelligent stress resistance, it provides new insights for the high efficient production of plant flavonoids and other plant natural products in MCFs.

TL;DR: Wang et al. as mentioned in this paper reported the development of an efficient C. glutamicum strain to produce 1,3-propanediol from glucose and xylose by systems metabolic engineering approaches.

TL;DR: In this article , the authors explore the metabolic bottlenecks of the microbial communities adopting latest advances of profiling and 13C tracer-based analysis using state-of-the-art analytical platforms (GC, GC-MS, LC-MS and NMR).

TL;DR: In this article , metabolic engineering of Corynebacterium glutamicum was reported to efficiently secrete recombinant spidroins. But, the results were limited to a single strain.

TL;DR: In this paper , the authors demonstrate that the recently added NMR data modeling capabilities of INCA 2.0 can enable entirely new experimental designs that lead to improved flux resolution and can be applied to a wide range of biological systems and measurement time courses.

TL;DR: In this article , the 1-Deoxy-D-xylulose 5-phosphate synthase (DXS) was used as the main rate-limiting enzyme of the MEP pathway and overexpression of diterpene synthase fusion proteins increased the production of high-value diterpenoids.

TL;DR: In this article , a machine-learning approach was applied to uncover the genome-scale transcriptional regulatory network (TRN) in Pseudomonas putida KT2440, an important organism for bioproduction.

TL;DR: In this paper , a multilevel metabolic engineering strategy was employed for the high-level production of 2-phenylethanol (2-PE) by combining optimization of Ehrlich pathway, central metabolic pathway, and phenylpyruvate pathway.

TL;DR: In this article , the authors compared three Pseudomonas putida KT2440 (P. putida) strains engineered to convert lignin-related aromatic compounds to muconic acid (MA), showing that expression of PraI leads to lower 4-HBA accumulation and decreased NADP+/NADPH ratios relative to strains harboring pobA.

TL;DR: In this paper , a Pseudomonas taiwanensis strain was used for the conversion of cyclohexane to adipic acid (AA), achieving a maximal activity of 22.6 ± 0.2 U gCDW-1 and a titer of 10.2 g L-1.

TL;DR: In this paper , four different synthetic metabolic modules for biosynthesis of short-to medium-chain length hydrocarbons in the model cyanobacterium Synechocystis sp.

TL;DR: In this article , the authors discuss metabolic engineering efforts to achieve economically feasible industrial production of fuels and chemicals from microbial cell factories using the carbohydrate portion of lignocellulosic biomass as substrates.

TL;DR: In this article , the metabolic carbon tradeoff between starch and lipid was studied using 13CO2-labeling experiments and isotopically nonstationary metabolic flux analysis, not previously applied to the mature leaves of a crop.

TL;DR: A review of recent progress made toward synthetic methylotrophy (including methanotrophic) is discussed in this paper , where the importance of amino acid metabolism and alternative one-carbon assimilation pathways are detailed.

TL;DR: In this article, the authors used reverse genetic engineering of single-nucleotide polymorphisms occurring in the genes encoding for phosphoribulokinase and nicotinic acid mononucleotide adenylyltransferase after evolution, and verified their influence on the improved autotrophic phenotypes.

TL;DR: In this paper , the L-cysteine-responsive transcriptional regulator CcdR was used to develop a genetically encoded biosensor for engineering and screening the overproducer.

TL;DR: In this paper , the authors used reverse genetic engineering of single-nucleotide polymorphisms occurring in the genes encoding for phosphoribulokinase and nicotinic acid mononucleotide adenylyltransferase after evolution, and verified their influence on the improved autotrophic phenotypes.

TL;DR: In this paper , the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars induced profound metabolic alterations in carotensoid, apocarotenoids and phytohormones pathways, leading to an up to 77% fruit yield increase and enhanced fruit's provitamin A content.

TL;DR: In this paper , a cascaded value chain that provides green polyethylene terephthalate (PET) from lignin, the world's most underutilized renewable, via fermentative production of cis, cis-muconate (MA) from Lignin-based aromatics was demonstrated.

TL;DR: In this article, the sesquiterpenoid genes in holy basil (Ocimum sanctum) were systematically screened using a precursor-providing yeast system, and the enzymes that synthesize β-caryophyllene and its close isomer α-humulene were successfully identified.

TL;DR: In this article , the potential of C. necator to generate sugar alcohols from CO2 using precursors derived from the CBB cycle is highlighted for the first time.

TL;DR: In this paper , a yeast de novo synthesis of Diosgenin (DSG) was achieved using glucose as the carbon source, but DSG production was only at the milligram level, which is too low for industrial production.

TL;DR: In this article , the sesquiterpenoid biosynthesis genes in Holy Basil (Ocimum sanctum) were systematically screened using a precursor-providing yeast system, and the enzymes that synthesize β-caryophyllene and its close isomer α-humulene were successfully identified.

TL;DR: In this paper, the authors apply a genome-scale metabolic model of the cyanobacteria Synechococus sp. PCC 7002 to identify strain designs accounting for this unique metabolism that are predicted to improve the production of various biofuel alcohols synthesized via an engineered biosynthesis pathway.