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Showing papers in "Process Biochemistry in 1991"


Journal ArticleDOI
TL;DR: In this paper, the effect of xylose concentration upon fermentation was determined for Pichia stipitis and three other yeasts, and an effective method of hydrolysate treatment employing neutralization with NaOH and activated charcoal was established.

144 citations


Journal ArticleDOI
TL;DR: The relative contributions from strain development, nature of substrate, substrate concentration and cultivation conditions on the improvements in enzyme production with Trichoderma species were found to be of the same order of magnitude.

140 citations


Journal ArticleDOI
TL;DR: The authors reviewed different types of bioreactor which have been described and used for various purposes, incorporating several modification for improved operation and performance for solid-state fermentations, which have gained importance recently due to several advantages over submerged fermentations.

102 citations


Journal ArticleDOI
TL;DR: In this article, a kinetic study on the anaerobic digestion of olive mill wastewater (OMW) was carried out in a semicontinuous, well-stirred fermenter with microorganisms immobilized on clayey supports.

66 citations


Journal ArticleDOI
TL;DR: Results of many investigation show that the use of micro gel beads is a very practical and effective method of overcoming the problem of providing sufficiently high oxygen transfer rates to the immobilized cells.

57 citations


Journal ArticleDOI
TL;DR: In this paper, β-Galactosidase was attached through its amine groups to aldehyde-glass, and the intrinsic kinetic constants (V max and K M ) and coupling parameters for the immobilization were calculated.

57 citations


Journal ArticleDOI
TL;DR: In this paper, a review examines data on the functions of essential metal ions and their role in metalloenzymes and a calculation to determine the balanced growth media is discussed.

53 citations


Journal ArticleDOI
TL;DR: In this paper, the three major ways to describe liquid chromatography are presented, i.e., the continuity equations, the theory of interferences and the plate theory, and the numerical ways of solving the differential equations stated by the different models are also considered.

50 citations


Journal ArticleDOI
TL;DR: Mannitol was a preferred substrate and batch growth on mannitol followed by fed-batch DHA production on glycerol avoided the inhibitory effects due to substrate and product and led to an optimised D HA production process.

48 citations


Journal ArticleDOI
TL;DR: In this paper, the cellulases induced by 1 g partially saccharified newspaper were used for cellulase production, and a total yield of 10−11 g fermentable sugars was obtained, from a total of 16 g office paper.

41 citations


Journal ArticleDOI
TL;DR: In this paper, the canteen and mess wastes (CMW) were used for biogas generation in a 200 l Khadi and Village Industries Commission of India digester and a high gas yield of 0·981 m3 kg−1 volatile solids (VS) with a methane content of 50% was obtained.

Journal ArticleDOI
TL;DR: In this paper, the authors used a pulsed plug flow extractor to extract amyloglucosidase from mouldy bran obtained from a solid-state fermentation system.

Journal ArticleDOI
Takashi Hamada1, Misao Sugishita1, Yaichi Fukushima1, Tetsuro Fukase, Hiroshi Motai1 
TL;DR: The soy sauce made by the bioreactor system was similar to a conventional one in regard to chemical components and the quality of flavour.

Journal ArticleDOI
TL;DR: A pilot-scale production process for a recombinant protein including fermentation, cell harvest, disintegration, extraction, diafiltration and freeze-drying are described, yielding an overall recovery of 37%.

Journal ArticleDOI
TL;DR: In this paper, two downflow reed beds, with two treatment stages, have been added in front of a previously installed horizontal flow bed on a dairy farm to treat parlour washings and standing yard wastewater.

Journal ArticleDOI
TL;DR: In this article, individual and combined effect of five minerals, which exist as the major part of ash in the milk and whey were studied as they affect the gross yield of lactose crystallization.

Journal ArticleDOI
TL;DR: In this article, the pH and temperature optima of these arabinoxylan-degrading enzymes were determined and β-xylanase, p- nitrophenyl xylosidase, α- l -arabinofuranosidase and acetyl esterase remained stable at 55°C for h.

Journal ArticleDOI
TL;DR: Yeast extract had a significant effect on the final concentration and the final yield of the acid per unit of substrate consumed but not on the product formation rate, and the addition of K2HPO4 did not improve significantly the production of propionic acid.

Journal ArticleDOI
TL;DR: Protease inhibition by the addition of phenylmethyl sulphonyl flouride resulted in enhanced activities of ligninase and amylase beyond the 9th day, suggesting an effect of protease on lignInase andAmylase activity.

Journal ArticleDOI
TL;DR: Studies using the coacervate capsules for plant cell immobilization showed that plant cells were more viable in the water soluble chitosan salt containing coacovate capsules than in acid soluble ch itosan salts containing coACervate Capsule.

Journal ArticleDOI
TL;DR: In this article, a comparative study between a CSTR with cell recycle and an air-lifted CSTR was carried out in 30 l capacity reactors for metal solubilization from anaerobically digested sludge.

Journal ArticleDOI
TL;DR: In fed-batch culture, the enzymes tested, namely avicelase, CMCase, cellobiase and xylanase showed significant improvements over batch fermentations with regard to enzyme titres and productivities.

Journal ArticleDOI
Emilio Molina1, E. Martínez1, S. Sańchez1, F. García1, A. Contreras1 
TL;DR: The influence of both the initial N:P ratio and temperature on the growth of Tetraselmis sp.

Journal ArticleDOI
TL;DR: Saccharomyces cerevisiae was cultivated using by-products and wastes of potato and wheat starch production in batch and continuous cultures and the performance of continuous cultures is considerably better than that of batch cultures.

Journal ArticleDOI
TL;DR: In this article, two multistage methods of sugar extraction from carob pods were compared with a single stage extraction/pressing process, and a comparison of the calculated diffusion coefficients showed that the enhanced yield in the recycle flow process resulted from a decrease of a diffusion limitation observed in static operation.

Journal ArticleDOI
TL;DR: In this article, a model which predicts the filtrate flux and the pressure drop over the length of a cross-flow hollow-fibre microfilter is presented, and data from a series of 128 experimental runs were fitted using a multi-variable, non-linear regression program.

Journal ArticleDOI
TL;DR: Cyclosporin is unique among the presently available immunosuppressive drugs in that it reversibly inhibits only some classes of lymphocytes and does not affect haemopoietic tissues (bone marrow).

Journal ArticleDOI
TL;DR: The mechanical resistance of the coated beads was higher and the biomass leakage lower than with uncoated controls while the bioactivity and the mass transfer resistance were similar, suggesting that coated beads may be of use in bioreactor systems.

Journal ArticleDOI
TL;DR: In this article, the malting loss increased with days of germination but decreased with increased concentration of potassium bromate, and a concentration of 125 mg/litre of Bromate was found to be optimal after 5 days of malting.

Journal ArticleDOI
TL;DR: Fermentation media containing different concentrations of glycerol were used in batch and continuous fermentations to investigate the effect of increasing broth osmolality on growth and fermentation rates of Zymomonas mobilis at the high operational temperature of 40 °C and immobilized cells showed a lower threshold inhibition level for both growth and productivity when compared with free cells, but a higher tolerance for Osmolalities above 5·0 os/kg.