Showing papers in "Revista Brasileira De Parasitologia Veterinaria in 2007"
TL;DR: The parasite communities of the studied fishes showed scarcity of significant correlations of parasitic abundance, species richness and diversity with the size of the host, and could be originated by the oligotrophic characteristics of the Lajes Reservoir.
Abstract: Entre abril de 2002 e julho de 2003, foram necropsiados 231 especimes de peixes: 39 Astyanax bimaculatus (Linnaeus, 1758), 79 A. fasciatus (Cuvier, 1819), 23 Hypostomus affinis (Steindachner, 1877), 26 Hoplias malabaricus (Bloch, 1794), 30 Loricariichthys castaneus (Castelnau, 1855) e 34 Trachelyopterus striatulus (Steindachner, 1876) provenientes do Reservatorio de Lajes (22o42' - 22o50'S, 43o53' 44o05'O), Estado do Rio de Janeiro, Brasil, para estudo das suas comunidades parasitarias. A maioria dos especimes de H. affinis (95,6%) e H. malabaricus (84,6%) estavam parasitados por pelo menos uma especie de metazoario. Em A. bimaculatus, A. fasciatus, L. castaneus e T. striatulus 41%, 39,2%, 56,7% e 14,7% dos especimes estavam parasitados, respectivamente. Foram coletadas oito diferentes especies de metazoarios parasitos: 2 em A. bimaculatus, 3 em A. fasciatus, 3 em H. affinis, 4 em H. malabaricus, 4 em L. castaneus e 1 em T. striatulus. As comunidades de metazoarios parasitos das seis especies de peixes estudadas apresentaram tipico padrao de distribuicao agregada. Foram observados dois casos de correlacao negativa entre a abundância e a prevalencia parasitarias e o comprimento total dos hospedeiros. A comunidade parasitaria de L. castaneus apresentou os maiores valores de intensidade media, indice de dispersao e diversidade. As comunidades parasitarias dos peixes estudados apresentaram escassez de correlacao entre a abundância, riqueza parasitaria e diversidade com o comprimento total dos hospedeiros. Os baixos valores de riqueza e diversidade das comuni-dades parasitarias podem ser atribuidos as caracteristicas oligotroficas do Reservatorio de Lajes.
39 citations
TL;DR: The results revealed the presence of infection by N. caninum in the herds investigated, drawing attention to its role as a potential cause of abortion in cattle in Mato Grosso do Sul.
Abstract: Neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, as well as ruminants and equines. It was described in 1988 and has been known as a major cause of abortion in bovines and neuromuscular alterations and death in dogs. To estimate the prevalence of bovine neosporosis in the 22 municipalities of the so-called Estrato 1 subregion of the Brazilian state of Mato Grosso do Sul, blood samples were collected from cows aged 24 months and older, from December 2003 to March 2004. During sample collection, a questionnaire was used to gather data of epidemiological interest. The samples were subjected to serological diagnosis (indirect fluorescence antibody test - IFAT). Prevalences of 14.9% (449/2488) and 69.8% (143/205) were found for the animals and herds sampled, respectively. The variable found to be associated with seropositivity to N. caninum was abortion (OR 2.52; CI 1.25-5.06). The results revealed the presence of infection by N. caninum in the herds investigated, drawing attention to its role as a potential cause of abortion in cattle in Mato Grosso do Sul.
36 citations
TL;DR: In order to detect the positivity to antigens of Dirofilaria immitis, antibodies anti-Borrelia burgdorferi and anti-Ehrlichia canis, 200 canine blood samples were collected as followed: 100 from the municipality of Ilheus and 100 from Itabuna, State of Bahia.
Abstract: In order to detect the positivity to antigens of Dirofilaria immitis, antibodies anti-Borrelia burgdorferi and anti-Ehrlichia canis, 200 canine blood samples were collected as followed: 100 from the municipality of Ilheus and 100 from Itabuna, State of Bahia. The kit Snap 3DX (IDEXX Laboratories) was used to performe serology. None of the tested animals were positive for antigens of D. immitis. Only two dogs of them were positive for antibodies anti-B. burgdorferi. From all the samples analyzed, 72 (36%) were positive for antibodies anti-E. canis, 43 from Ilheus and 29 from Itabuna (p=0,027).
35 citations
Journal Article•
TL;DR: In this paper, the authors evaluated sero-occurrence of toxoplasmosis in swine and ovine slaughtered in the municipality of Guarapuava in the state of Parana.
Abstract: This study evaluated sero-occurrence of toxoplasmosis in swine and ovine slaughtered in the municipality of Guarapuava in the state of Parana. Blood samples ...
30 citations
Journal Article•
TL;DR: Univ Estadual Paulista, UNESP, Dept Apoi Producao & Saude Anim, Curso Med Vet, BR-16050680 Aracatuba, SP, Brazil
Abstract: Univ Estadual Paulista, UNESP, Dept Apoi Producao & Saude Anim, Curso Med Vet, BR-16050680 Aracatuba, SP, Brazil
24 citations
TL;DR: The profile of insecticide use helps to explain the widespread resistance of horn flies to pyrethroids in the state of Mato Grosso do Sul as well as their high susceptibility to the organophosphate.
Abstract: Horn fly susceptibility to insecticides was evaluated in the state of Mato Grosso do Sul, Brazil, from October 2000 to September 2002. Insecticide bioassays (n=57) were conducted in 38 ranches from 14 municipalities throughout the state. Horn flies from wild populations were collected on cattle and exposed to filter papers impregnated with cypermethrin, permethrin, or diazinon and mortality was assessed after two hours. Resistance to cypermethrin was detected in all populations, with resistance ratios (RR) ranging from 27.6 to 91.3-fold. Permethrin bioassays provided apparently low levels of resistance (RR<5), however, resistant flies were found in 96.9% of the populations based on diagnostic concentrations. From both pyrethroid bioassays, resistance was detected in 97.4% of the populations. On the other hand, a high susceptibility to diazinon (RR< or =1.1) was detected in all populations. Pyrethroid products, most cypermethrin (92.3%) and deltamethrin (66.7%), were used in all ranches controlling horn flies (97.5%). Insecticide treatments, usually incorrectly applied, were routinely delivered by manual backpack sprayers in most ranches (84.5%). This profile of insecticide use helps to explain the widespread resistance of horn flies to pyrethroids in the state as well as their high susceptibility to the organophosphate. Inadequate control practices contribute to aggravate the resistance problem and its consequences.
24 citations
Journal Article•
TL;DR: In this paper, the apaiari Astronotus ocellatus (Cope, 1872) from Guandu river, (22°48'32"S, 43o37'35"W), state of Rio de Janeiro, Brazil were studied.
Abstract: Thirty five specimens of apaiari Astronotus ocellatus (Cope, 1872) from Guandu river, (22°48’32"S, 43o37’35"W), state of Rio de Janeiro, Brazil were studied ...
22 citations
Journal Article•
TL;DR: Evaluating in vitro the action of eight chemical principles by disinfection efficacy of Eimeria tenella oocysts found the best DE were observed in T3, T5, T4 and T8 treatments.
Abstract: The objective of this study was to evaluate in vitro the action of eight chemical principles by disinfection efficacy (DE) of Eimeria tenella oocysts. Disinfection efficacy was evaluated by either destruction or sporulation inhibition of the oocysts. Eight treatments were performed: T1 (Glutaraldehyde 42.5 g + Benzalkonium Chloride 7.5 g); T2 (Benzalkonium chloride + quaternary ammonium salt); T3 (formol 37% + Sodium Dodecylbenzene Sulfonate 12%); T4 (sodium hypochlorite 2%); T5 (Orthodichlorobenzene 60% + Xylene 30%); T6 (Polyoctyl polyamino ethyl glycine + Polyoxyethylene alkylphenol ether + Sodium Chloride); T7 (Chloramine T) and finally T8 (free iodine 2.25% + Phosphoric acid 15 g). The control test was carried out with distilled water (T9). The best DE were observed, respectively, in T3 (79.49%), T5 (75.60%) and T4 (65.56%) treatments.
22 citations
Journal Article•
TL;DR: Study results show that migration of T. colubriformis larvae was more influenced by weather conditions than by forage species, and infected larvae recovery was similar among the different strata during spring.
Abstract: 1 ABSTRACT:- ROCHA, R.A.; BRICARELLO, P.A.; ROCHA, G.P.; AMARANTE, A.F.T. (Trichostrongylus colubriformis larvae recovery from different Brachiaria decumbens and Panicum maximum strata). Recupe- racao de larvas de Trichostrongylus colubriformis em diferentes estratos de Brachiaria decumbens e Panicum maximum. Revista Brasileira de Parasitologia Veterinaria, v. 16, n. 2, p. 77-82, 2007. Departamento de Parasitologia, Instituto de Biociencias,UNESP. Caixa Postal 510, Botucatu, SP, 18618-000 Brazil. E-mail: rrabdallah@hotmail.com The purpose of the experiment was to evaluate infective Trichostrongylus colubriformis larvae vertical migration in two forage grass species. Experimental modules formed by eight plots, established with Brachiaria decumbens cv. Australian and Panicum maximum cv. Aruana, were used in the study, totaling four plots for each grass species. Each plot was divided into six 30 x 30 cm subplots. Larval migration was evaluated in the four seasons of the year, in different plant strata (0-7, 7-14, 14-21, 21-28 and above 28 cm). Four feces deposits were made, one in each season of the year, in the middle of 30-cm tall forage. The feces were collected from the forage ten days after each feces deposit in the experimental subplots. Grass height was measured in each of the strata immediately before the collections. The forage of the different strata was cut from an area measuring 10-cm in radius. The feces were collected manually from the subplots. There was a grass species and grass stratum interaction in the deposit made in autumn (P<0.05). During that season, most of the larvae were recovered from the Brachiaria grass base; meanwhile, at the forage apex, the biggest average was registered in the aruana grass. Infective larvae (L3) recovery was similar among the different strata during spring. In springtime, the biggest L3 recovery occurred at the 21-28 cm stratum from both forage species. No L3 was recovered from any of the No L3 was recovered from any of the grass strata during winter and summer. Study results show that migration of T. colubriformis larvae was more influenced by weather conditions than by forage species.
20 citations
Journal Article•
TL;DR: The presence of Trypanorhyncha metacestodes in the muscles does not represent a risk of infection for humans, but they have a negative effect on fish aesthetics because of the repugnant aspect, causing consumer rejection and the prohibition for commercial purpose by sanitary inspectors.
Abstract: The aim of this study was to determine the cestodes species of the order Trypanorhyncha that parasite the blackfin goosefish, Lophius gastrophysus Miranda-Ribeiro, 1915 purchased from fishermen, markets and exportation
fish industries from municipalities of Cabo Frio, Niteroi, Duque de Caxias and Rio de Janeiro, emphasizing their parasite indexes, sites of infection and higienic-sanitary importance. Eighty seven specimens of L. gastrophysus were necropsied from March 2000 to December 2005. Forty one fish (47%) were parasitized by metacestodes of Trypanorhyncha. The collected species were Tentacularia coryphaenae, Nybelinia sp., Mixonybelinia sp. and Progrillotia dollfusi. Most of the parasitized fish (92.7%), presented metacestodes of Trypanorhyncha, in the abdominal
muscles and in muscles of commercial importance (lateral back-dorsum), belonging to the species T. coryphaenae, Nybelinia sp., Mixonybelinia sp. Only three (7.3%) fish specimens did not present trypanorhynch metacestodes in the muscles, two of them were parasitized by Nybelinia sp. on the mesentery and the celomatic cavity and the other by P. dollfusi on the celomatic cavity. On some individuals, Mixonybelinia sp. caused equimotic hemorragic lesions on the fascia of the abdominal muscles. The presence of Trypanorhyncha metacestodes in the muscles does not represent a risk of infection for humans, but they have a negative effect on fish aesthetics because of the repugnant aspect, causing consumer rejection and the prohibition for commercial purpose by sanitary inspectors. Parasites of the order Trypanorhyncha are recorded in L. gastrophysus for the first time.
17 citations
TL;DR: The stable fly, Stomoxys calcitrans, has a great importance in medical and veterinary health due to its feeding and reproductive habits and can disseminate some pathogenic agents among hosts even at long distances.
Abstract: The stable fly, Stomoxys calcitrans, has a great importance in medical and veterinary health due to its feeding and reproductive habits, which can disseminate some pathogenic agents among hosts even at long distances. This study aimed to describe, for the first time in Brazil, the bacterial microbiota in segments of the stable fly. Bacterial species were isolated from three different segments (cuticle, mouth parts and abdominal alimentary tract) of the stable fly. Twenty dairy farms were visited in four municipalities: Barra Mansa, Quatis, Resende and Rio Claro in the State of Rio de Janeiro in order to collect 20 flies in each site. Dissection of the flies and procedures of isolation and identification of bacterial species were performed. A total of 161 colonies of 33 distinct species were isolated, such as Escherichia coli, Staphylococcus aureus, and S. intermedius. Stomoxys calcitrans may harbor bacterial agents on their cuticle, mouth parts and abdominal alimentary tract and these bacteria may be pathogenic to their hosts.
Journal Article•
TL;DR: The mortality of tick nymphae treated with Beauveria bassiana and Metarhizium anisopliae formulations in laboratory conditions was evaluated and could be a viable alternative for the control of this tick in permanent preserved areas.
Abstract: 3 ABSTRACT:- LOPES, R.B.; ALVES, S.B.; PADULLA, L.F.L.; PEREZ, C.A. (Efficiency of Beauveria bassiana and Metarhizium anisopliae formulations on Amblyomma cajennense (Fabricius, 1787) nymphae). Eficiencia de formulacoes de Beauveria bassiana e Metarhizium anisopliae para o controle de ninfas de Amblyomma cajennense (Fabricius, 1787). Revista Brasileira de Parasitologia Veterinaria, v. 16, n. 1, p. 27-31, 2007. Departamento de Entomologia, Fitopatologia e Zoologia Agricola Escola Superior de Agricultura Luiz de Queiroz, Universidade do Estado de Sao Paulo, Avenida Padua Dias, 11, Caixa Postal 9, Piracicaba, SP 13418-900, Brazil. E-mail: sebalves@esalq.usp.br Amblyomma cajennense is a vector of several infectious diseases on mammals and became a serious problem in some localities in Brazil. Entomopathogenic fungi are very important biocontrol agents and could be a viable alternative for the control of this tick in permanent preserved areas. The aim of this study was to evaluated the mortality of tick nymphae treated with Beauveria bassiana and Metarhizium anisopliae formulations in laboratory conditions. Non fed nymphae were inoculated with 2x10 7 and 4x10 7 conidia/ml suspension of B. bassiana and 1x10 7 , 1.5x10 7 , 2x10 7 , 3x10 7 and 5x10 7 conidia/ml suspension of M. anisopliae. nymphae mortality found were 57.1 and 56.2% at the concentrations of 3x10 7 and 5x10 7 conidia/ml respectively, three days after treatment. After six days, the mortalities reached 100% for both highest concentrations and were between 25 and 35% for the others M. anisopliae treatments. The mortality caused by M. anisopliae at the concentrations of 1x10 7 , 1.5x10 7 and 2x10 7 conidia/ml reached 60.6, 64.1 and 66.8% respectively, after ten days. B. bassiana formulation caused mortality only at the highest concentration. Oil formulation of M. anisopliae is an ecological option for tick control in areas where the use of chemical pesticides may cause undesirable impact.
Journal Article•
TL;DR: During the study of the metazoan parasites of fishes from Araguaia River, municipality of Araguatins, Brazil, a third stage larvae of an undescribed species of Terranova were collected from the mesenteries of Plagioscion squamosissimus, marking the first record and description of larval of species of Martianova parasitic in South American freshwater fishes.
Abstract: During the study of the metazoan parasites of fishes from Araguaia River, municipality of Araguatins (05 degrees 39'S, 48 degrees 07'W), State of Tocantins, Brazil, a third stage larvae of an undescribed species of Terranova were collected from the mesenteries of Plagioscion squamosissimus. These larvae were characterized to present large size, excretory pore situated near of the base of ventrolateral lips, presence of short intestinal caecum dorsal to oesophagus, ventriculus less than seven times as long as wide and absence of mucron. This is the first record and description of larval of species of Terranova parasitic in South American freshwater fishes.
Journal Article•
TL;DR: In this paper, the authors evaluated the biochemical changes in rabbits experimentally infected by trypanosome evansi and found that six male rabbits were infected with the virus and only one died.
Abstract: The purpose of the present paper has been evaluating the biochemical changes in rabbits experimentally infected by Trypanosome evansi. Six male rabbits, sepa...
TL;DR: After decades without new registers, the occurrence of the ticks Amblyomma obolongoguttatum Koch, 1844, A. brasiliense Aragao, 1908, and A. naponense, 1869 are confirmed in the State of Espirito Santo, Brazil.
Abstract: We confirm, after decades without new registers, the occurrence of the ticks Amblyomma obolongoguttatum Koch, 1844, A. brasiliense Aragao, 1908, and A. humerale Koch, 1844 in the State of Espirito Santo, Brazil, besides the first record of A. naponense (Packard, 1869) in this state.
TL;DR: The flies were more abundant in February and March and the most attractive bait was fish, while Chrysomya megacephala and Lucilia eximia were the most frequent flies.
Abstract: It was carried out a survey of blowflies in a area of the Campus (Valonguinho) of the Universidade Federal Fluminense, Niteroi, Rio de Janeiro. The collections were performed with traps, using baits of fish (sardine), bovine liver, shrimps and banana. Were collected 6015 flies, Chrysomya megacephala and Lucilia eximia were the most frequent (50.55% and 21.52%, respectively). The flies were more abundant in February and March and the most attractive bait was fish (38.32%).
TL;DR: The present study confirms the ubiquity of P. simicola in captive born and raised rhesus monkeys that would compromise experimental studies involving the respiratory system.
Abstract: Histological lesions in the lungs of rhesus monkeys (Macaca mulatta) related with Pneumonyssus simicola were evidenced. The most prominent pathologic alterations included numerous thin-walled cysts 1-5 mm diameter scattered throughout the lungs, bronchiolitis, peribronchiolitis where the mites were found associated with pigmented and non-pigmented materials. Our study included data from 347 rhesus monkeys submitted to necropsies during 20 years. Four adult debilitated animals were found with pulmonary acariasis which showed a very low incidence of parasite (1.2%) in the colony. Most of the published literature described as common and widespread pulmonary acariasis in Old World monkeys. The present study confirms the ubiquity of P. simicola in captive born and raised rhesus monkeys that would compromise experimental studies involving the respiratory system.
TL;DR: The results indicate that the mite L. radovskyi is endemic in this population of cats from Recife Metropolitan Region, State of Pernambuco, Brazil, during the period of May through December of 2003.
Abstract: This study was performed with the aim of to investigate the infestation by the cat fur-mite Lynxacarus radovskyi in cats from Recife Metropolitan Region, State of Pernambuco, Brazil, during the period of May through December of 2003. Hair samples were taken from cats of both sexes and different ages and submitted to microscopical examination. The results showed that 75.84% (254/335) of cats were infested with L. radovskyi, but statistically significant differences were not observed among the presence of mite and sex, race and age. The results indicate that the mite L. radovskyi is endemic in this population.
Journal Article•
TL;DR: Eighty and one specimens of Peprilus paru (Linnaeus,1758) (Perciformes: Stromateidae), collected from the coastal zone of the State of Rio de Janeiro, Brazil...
Abstract: Eighty and one specimens of Peprilus paru (Linnaeus,1758) (Perciformes: Stromateidae), collected from the coastal zone of the State of Rio de Janeiro, Brazil...
Journal Article•
TL;DR: Pesca, APTA, SAA, Nucl Pesquisa Desenvolvimento Litoral Norte, BR-11680000 Ubatuba, SP, Brazil
Abstract: Inst Pesca, APTA, SAA, Nucl Pesquisa Desenvolvimento Litoral Norte, BR-11680000 Ubatuba, SP, Brazil
Journal Article•
TL;DR: In this paper, the occurrence of the bovine parasitic otitis caused by rhabditiformnematodes in Gyr cows breed from the State of Rio de Janeiro, Brazil was described.
Abstract: The aim of this report was to describe the occurrence of the bovine parasitic otitis caused by rhabditiformnematodes in Gyr cows breed from the State of Rio ...
Journal Article•
TL;DR: In this article, the authors evaluated the in vitro effects of poison therapy on parasitism control for various animal species, including mice, rats, birds, and amphibians, in vitro.
Abstract: Phitotherapy has been frequently utilized in parasitism control for numerous animal species. The aim of this experiment was to evaluate the in vitro effects ...
TL;DR: Isospora vanriperorum from the green-winged saltator (Saltator similis) is described in Southeastern Brazil, with prominent Stieda body, barely discernible substiedaBody and residuum centered and granulated.
Abstract: Isospora vanriperorum from the green-winged saltator (Saltator similis) is described in Southeastern Brazil Oocysts are spherical to subspherical, 194-26 by 183-26μm (231 by 224μm), shape index 104 with a single layered wall, fine, smooth and yellowish Micropyle and residuum are absents, but one elliptical polar granule is present Sporocysts are ovoid, 145-202 by 81-125μm (163 by 108μm), shape index 153 with prominent Stieda body, barely discernible substieda body and residuum centered and granulated
TL;DR: Adult specimens of Paratanaisia bragai collected from renal ducts of Columba livia naturally infected, were fixed in glutaraldehyde 2.5 degrees and processed for scanning electron microscopy to show in details the oral sucker subterminal and bifid and simple scales.
Abstract: Adult specimens of Paratanaisia bragai collected from renal ducts of Columba livia naturally infected, were fixed in glutaraldehyde 2.5 degrees and processed for scanning electron microscopy. Two distinct types of scales had been identified, bifid and simple scales, disposed in transversal rows, on the tegument of dorsal and ventral face of the body. Also show in details the oral sucker subterminal, well developed and acetabulum of size reduced, located in third medium of the body, surrounded for small protuberance and four papillae on its edge.
TL;DR: The results showed that B. microplus females fed on dogs to complete their life cycle and were able to ovoposite viable eggs suggesting that dogs can be a possible alternative hosts to B.microplus, especially when there is no other preferential host species available.
Abstract: 3 ABSTRACT:- FRANQUE, M. P.; SANTOS, H. A.; SILVA, G. V. O.; TAJIRI, J. T.; MASSARD, C. L. (Biological characteristics of Boophilus microplus (Acari: Ixodidae) on dog under experimental infestation.) Caracteris- ticas biologicas de Boophilus microplus (Acari: Ixodidae) a partir de infestacao experimental em cao. Revista Brasileira de Parasitologia Veterinaria, v. 16, n. 4, p. 238-242, 2007. Departamento de Parasitologia Animal, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Km 7 da BR 465, Seropedica, RJ 23890.000, Brazil. E-mail: carlosmassard@ufrrj.br Boophilus microplus, a common parasite of cattle, has eventually reported in dogs. To describe biological features of this parasitism, one dog was experimentally infested with 10,000 larvae of B. microplus which were previously held in acclimatized camera at 27 ±1o C and relative humidity up to 80%. The mean of parasitic phase was 24.4 ± 1.50 days, with 0.42% of recovery rate. Of 21 natural detached B. microplus females, six engorged enough (75.1 ± 30.23mg) to achieve posture. The mean period of pre-posture was 4.33 ± 1.37 days and the means period of posture was 9.17 ± 2.32, producing a mean of 18.78 ± 15.34 posture weight. The mean of eggs production index observed was 22.38%. The results showed that B. microplus females fed on dogs to complete their life cycle. The females collected were able to ovoposite viable eggs suggesting that dogs can be a possible alternative hosts to B. microplus, especially when there is no other preferential host species available.
TL;DR: The finding of this tick species (9 females) parasitizing lizard (Tupinambis teguixin) at the Municipality of Glorinha, State of Rio Grande do Sul confirms establishment of A. fuscum in the South of Brazil.
Abstract: Amblyomma fuscum known only from Brazil has been described as a rare tick species with few reports of its occurrence in South and Southeast region. This is a new records this tick species (9 females) parasitizing lizard (Tupinambis teguixin) at the Municipality of Glorinha, State of Rio Grande do Sul. The females were deposited in the tick collection of Veterinary Research Institute Desiderio Finamor (7 specimens), Eldorado do Sul, RS and in the Acari collection from Instituto Butantan, Sao Paulo, State of Sao Paulo (2 specimens). The finding confirms establishment de A. fuscum in the South of Brazil.
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TL;DR: Investigating the possible role of BNC as phagocytic cells and its participation in the bovine transplacental infection of N. caninum may conclude that BNC could potentially participate in the transplacented infection of bovines neosporosis.
Abstract: 3 ABSTRACT:- MACHADO, R.Z.; MINEO, T.W. P.; LANDIM JR, L.P.; CARVALHO, A.F.; SOLANGE M. GENNARI; MIGLINO, M.A.A. Possible role of bovine trophoblast giant cells in transplacental transmission of Neospora caninum in cattle. (Um possivel envolvimento de celulas gigantes trofoblasticas na transmissao transplacentaria de Neospora caninum em bovinos.) Revista Brasileira de Parasitologia Veterinaria, v. 16, n. 1, p. 21-25, 2007. Laboratorio de Imunoparasitologia, Departamento de Patologia Veterinaria, FCAV/UNESP, Jaboticabal, SP, 14.884-900, Brasil. E-mail: zacarias@fcav.unesp.br Neospora caninum is an aplicomplexan parasite that has brought several concerns to cattle raisers worldwide due to its relationship to fetal loss. However, the mechanism of the parasite's transplacental infection and induced abortions are not completely understood. Bovine trophoblastic binucleated cells (BNC) play a major role in the maternal-fetal interactions, migrating during the entire pregnancy from chorionic connections to uterine epithelium. This study aimed to investigate the possible role of BNC as phagocytic cells and its participation in the bovine transplacental infection of N. caninum. BNC was isolated by discontinuous Percoll gradient, and characterized by Hoeschst 33342 nucleus-specific staining. Isolated BNC were cultured in DMEM supplemented with 10% bovine fetal serum, and infected with 10 4 tachyzoites of N. caninum NC-1 strain. Parasite invasion was visualized by indirect immunofluorescence and Giemsa technique. Multiplication of parasites took place in 2-3 day cycles. Healthy cows' placenta and normal and infected cultured BNC was immunostained with monoclonal antibodies against CD- 163, MAC-387 and iNOS, demonstrating their phagocyte capacity. Thus, BNC was characterized as cells with macrophagic activity, which may host N. caninum in vitro. Therefore, we may conclude that BNC could potentially participate in the transplacental infection of bovine neosporosis.
TL;DR: It was reported 33% of mortality during the total period analyzed, and the production of eggs and nutritional rates were decreased only for a short period after treatment, as well as the analysis of biology of tick's samples which were transferred to an incubation chamber.
Abstract: The aim of the present study was to evaluate the capacity of the fungus Metarhizium anisopliae to control Boophilus microplus tick in pen trials. Infested calves were held in individual pen and treated with fungus suspension through aspersion bath. The results were evaluated based on ticks' mortality rate for 28 days after treatment, and on the analysis of biology of tick's samples which were transferred to an incubation chamber. It was reported 33% of mortality during the total period analyzed, and the production of eggs and nutritional rates were decreased only for a short period after treatment.
TL;DR: Species of parasitoids associated with synanthropic flies were trapped by using shopped bovine liver as bites at slaughterhouse Itumbiara in the State of Goias, from March to December 2005, with overall prevalence of parasitism 15.3%.
Abstract: Especies de parasitoides associados com moscas sinantropicas foram coletadas com auxilio de iscas de figado bovino no matadouro Alvorada em Itumbiara, Estado de Goias, de marco a dezembro de 2005. As pupas foram removidas da areia, apos usar iscas de figado como substrato para atrair as moscas. Elas foram individualizadas em capsulas de gelatina ate a emergencia dos adultos de moscas ou de seus parasitoides. A prevalencia total de parasitismo foi 15,3%. A frequencia absoluta, porcentagem e especies de parasitoides coletados foram: 205 exemplares (2,3%) de Aphaereta sp., 173 (80,0%) de Brachymeria podagrica, 578 (15,7%) Nasonia vitripennis, um (0,5%) de Pachycrepoideus vindemmiae, dois (0,9%) de Spalangia sp. e um (0,5%) de Trybliographa sp. A porcentagem de parasitismo apresentada pelos parasitoides de Aphaereta sp., B. podagrica, N. vitripennis, P. vindemmiae, Spalangia sp., e Trybliographa sp. foi de 0,4%, 12,3%, 2,4%, 0,1%, 0,1% e 0,1%, respectivamente. As especies de dipteros parasitados foram: Chrysomya albiceps, C. megacephala, Musca domestica, Oxysarcodexia thornax, Peckia chrysostoma e Sarcodexia lambens.
TL;DR: Tyzzeria parvula from the greylag goose (Anser anser) is described in Southeastern Brazil containing numerous granules and spherules and surrounded by residuum there are eight sporozoites having one end round and other fine and slightly curved.
Abstract: Tyzzeria parvula from the greylag goose (Anser anser) is described in Southeastern Brazil. Oocysts are spherical to subspherical ranging from 12.4-180 to 10.7-15.9µm (15.4 X 13.4 µm), shape index 1.15, with a double layered wall 0.4 to 0.7 µm thick (n=20), outer smooth and colorless, inner pale greenish. Micropyle is absent, but oocyst residuum is present containing numerous granules and spherules. Surrounded by residuum there are eight sporozoites having one end round and other fine and slightly curved.