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Showing papers in "The Journal of Agricultural Science in 2014"


Journal ArticleDOI
TL;DR: In this article, the functional and nutraceutical compounds present in common buckwheat (Fagopyrum esculentum) and tataricum (Tataricum) are reviewed.
Abstract: A growing trend for nutraceutical and gluten-free cereal-based products highlights the need for development of new products. Buckwheat is one of the potential candidates for such products and the present paper reviews the functional and nutraceutical compounds present in common buckwheat (Fagopyrum esculentum) and tartary buckwheat (Fagopyrum tataricum). The vital functional substances in buckwheat are flavonoids, phytosterols, fagopyrins, fagopyritols, phenolic compounds, resistant starch, dietary fibre, lignans, vitamins, minerals and antioxidants, which make it a highly active biological pseudocereal. Cholesterol-lowering effects that lessen the problems of constipation and obesity are important health benefits that can be achieved through the functional substances of buckwheat.

175 citations


Journal ArticleDOI
TL;DR: In this article, two different models (CAPRI and MITERRA) were used to quantify the N flows in agriculture in the European Union (EU27), at country-level and for EU27 agriculture as a whole, differentiated into 12 main food categories.
Abstract: Nitrogen (N) is an essential element for plants and animals. Due to large inputs of mineral fertilizer, crop yields and livestock production in Europe have increased markedly over the last century, but as a consequence losses of reactive N to air, soil and water have intensified as well. Two different models (CAPRI and MITERRA) were used to quantify the N flows in agriculture in the European Union (EU27), at country-level and for EU27 agriculture as a whole, differentiated into 12 main food categories. The results showed that the N footprint, defined as the total N losses to the environment per unit of product, varies widely between different food categories, with substantially higher values for livestock products and the highest values for beef (c. 500 g N/kg beef), as compared to vegetable products. The lowest N footprint of c. 2 g N/kg product was calculated for sugar beet, fruits and vegetables, and potatoes. The losses of reactive N were dominated by N leaching and run-off, and ammonia volatilization, with 0·83 and 0·88 due to consumption of livestock products. The N investment factors, defined as the quantity of new reactive N required to produce one unit of N in the product varied between 1·2 kg N/kg N in product for pulses to 15–20 kg N for beef.

133 citations


Journal ArticleDOI
TL;DR: In this article, the authors analyzed and characterized agro-climatic variability and changes and associated risks with respect to implications for rainfed crop production in the Central Rift Valley (CRV).
Abstract: Ethiopia is one of the countries most vulnerable to the impacts of climate variability and change on agriculture. The present study aims to understand and characterize agro-climatic variability and changes and associated risks with respect to implications for rainfed crop production in the Central Rift Valley (CRV). Temporal variability and extreme values of selected rainfall and temperature indices were analysed and trends were evaluated using Sen's slope estimator and Mann–Kendall trend test methods. Projected future changes in rainfall and temperature for the 2080s relative to the 1971–90 baseline period were determined based on four General Circulation Models (GCMs) and two emission scenarios (SRES, A2 and B1). The analysis for current climate showed that in the short rainy season (March–May), total mean rainfall varies spatially from 178 to 358 mm with a coefficient of variation (CV) of 32–50%. In the main (long) rainy season (June–September), total mean rainfall ranges between 420 and 680 mm with a CV of 15–40%. During the period 1977–2007, total rainfall decreased but not significantly. Also, there was a decrease in the number of rainy days associated with an increase (statistically not significant) in the intensity per rainfall event for the main rainy season, which can have implications for soil and nutrient losses through erosion and run-off. The reduced number of rainy days increased the length of intermediate dry spells by 0·8 days per decade, leading to crop moisture stress during the growing season. There was also a large inter-annual variability in the length of growing season, ranging from 76 to 239 days. The mean annual temperature exhibited a significant warming trend of 0·12–0·54 °C per decade. Projections from GCMs suggest that future annual rainfall will change by +10 to -40% by 2080. Rainfall will increase during November–December (outside the growing season), but will decline during the growing seasons. Also, the length of the growing season is expected to be reduced by 12–35%. The annual mean temperature is expected to increase in the range of 1·4–4·1 °C by 2080. The past and future climate trends, especially in terms of rainfall and its variability, pose major risks to rainfed agriculture. Specific adaptation strategies are needed for the CRV to cope with the risks, sustain farming and improve food security.

111 citations



Journal ArticleDOI
TL;DR: The results suggest the need for government to consider undertaking policies that will enhance farmers’ access to and control over land, that will provide farmers with improved access to agricultural implements like ploughs, and that will bring trading markets closer to farmers.
Abstract: Agriculture is vital to Zambia’s economic development and is a mainstay for the livelihoods of a large proportion of the population. Agricultural production is mainly dependent on rain-fed hoe cultivation with maize as the principal staple food crop. About 18 percent of national maize production comes from Zambia’s Southern province. In order to improve food security and minimize risks associated with heavy dependence on maize, the government of Zambia has been promoting crop diversification. This study analyzed the determinants of crop diversification as well as the factors influencing the extent of crop diversification by smallholder farmers in Southern province. The study used secondary data from the Central Statistical Office of Zambia. Results from a double-hurdle model analysis indicates that landholding size, fertilizer quantity, distance to market, and the type of tillage mechanism adopted have a strong influence on whether a farmer practices crop diversification. Our findings have important implications for policies that are designed to enhance crop diversification. In particular, our results suggest the need for government to consider undertaking policies that will enhance farmers’ access to and control over land, that will provide farmers with improved access to agricultural implements like ploughs, and that will bring trading markets closer to farmers.

68 citations


Journal ArticleDOI
TL;DR: The present results highlight the importance of considering varietal differences in crop pollination research and information on the pollination requirements of crop varieties is required by farmers to optimize management decisions in a world of increasing agropollination deficits.
Abstract: Winter oilseed rape (Brassica napus) is an important crop for human consumption and biofuel production and its production is increasing worldwide. It is generally assumed that cross-pollination by insects increases oilseed rape yield but testing of this has been restricted to a few rapeseed varieties and produced varying results. The present study determines whether cross-pollination benefits a number of oilseed rape varieties by comparing yield in the presence and absence of insects. Four rapeseed varieties (Sherlock, Traviata, Treffer and Visby) were used with ten individuals each in four pollination treatments: (1) supplementary hand-pollination, (2) open pollination with insects able to access the flowers, (3) wind pollination and (4) autonomous self-pollination. Across all four varieties, open and supplementary hand-pollination treatments resulted in higher fruit set, numbers of seeds per pod and seed yield compared with wind and self-pollination. The cross-pollination benefits, however, differed among rapeseed varieties: Treffer and Visby had a higher dependence on open (insects) and supplementary cross-pollination than Sherlock and Traviata. Across all four varieties, seed weight compensated for reduced fruit set and was highest when plants were self-pollinated. The present results highlight the importance of considering varietal differences in crop pollination research. Information on the pollination requirements of crop varieties is required by farmers to optimize management decisions in a world of increasing agropollination deficits.

67 citations


Journal ArticleDOI
TL;DR: In this paper, the authors identified some of the challenges faced by small-scale farmers in the Limpopo Province have been identified during the formal surveys and focus group meetings in the Tshakhuma, Rabali and Tshiombo areas were those linked to financial, assets, land ownership and biophysical factors.
Abstract: Macro- and micro-structural constraints, including those linked to and exacerbated by historical, natural and financial factors are some of the many stressors facing small-scale farmers in Limpopo Province. The challenge is to co-design ways to effectively manage these constraints with development actions. Small scale farmers in South Africa are still facing major challenges in the agricultural sector. In this paper some of the challenges faced by small-scale farmers in the Limpopo Province have been identified. Some of the challenges found during the formal surveys and focus group meetings in the Tshakhuma, Rabali and Tshiombo areas were those linked to financial, assets, land ownership and biophysical factors. Specific constraints included: (a) Market information and market access; (b) Price of inputs, for example fertilizer and herbicides; (c) Availability of inputs; (d) Irrigation; (e) Cost of transport, and Natural constraint.

65 citations


Journal ArticleDOI
TL;DR: In this paper, the authors used stochastic frontier analysis (SFA) and determined the factors that influence technical efficiency in maize production, including the age of a farmer, use of certified hybrid seed, access to loans and extension advice and off-farm income.
Abstract: Maize is the main staple food and cornerstone of Zambia’s agricultural economy and as such, high productivity and efficiency in its production are critical to food security and poverty reduction in the country. This paper estimates the technical efficiency of maize producers in Zambia using Stochastic Frontier Analysis (SFA) and also determines the factors which influence technical efficiency in maize production. Primary data from 400 households in the Central Province of Zambia were used in this study. Results show that there exists opportunities to increase technical efficiency from the present level of inputs. The average technical efficiency was at 50%, with a minimum of 2% and a maximum of 84%. The distribution of the technical efficiency is such that 14% of the farmers have efficiency scores that are less than 30% while 46% of the farmers have technical scores above 50%, and 14% have technical efficiency scores above 70%. Maximum likelihood results showed that the age of a farmer, use of certified hybrid seed, access to loans and extension advice and off-farm income influence technical efficiency. The study recommends Government and other maize stakeholders to devise strategies for improving access to credit and extension services and promoting use of certified hybrid seed as a way to improve the technical efficiency of maize producers in Zambia.

63 citations


Journal ArticleDOI
TL;DR: In this article, the authors evaluated the metabolic effect of nitrate in tomato seed germination by quantification of enzyme nitrate reductase (NR) activity, and also evaluated the activity of some antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX).
Abstract: Priming has been used to improve the performance of germination at the field, and potassium nitrate (KNO 3 ) is a promising compound for this purpose. The nitrate (NO 3 ) could be absorbed, being used in the metabolism of the embryo, through the enzyme nitrate reductase (NR). Besides, the priming could also activate the response of the antioxidant system, becoming the primed seeds more prepared for possible stresses. Thus, the aim of this study was to evaluate the metabolic effect of nitrate in tomato seed germination by the quantification of NR activity, and also evaluate the activity of some antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Tomato seeds were primed using solutions of polyethylene glycol (PEG 6000) -1,1MPa, 50 mM KNO 3 and PEG+KNO 3 . The variables analyzed were germination (germinability, mean germination time, mean germination rate, coefficient of variation of the germination time, uncertainty and synchrony) nitrogen, total proteins and enzymes. The germination data were analyzed using an ANOVA, comparing the averages by Scott-Knott test (P < 0.05). To analyze the nitrogen, protein and enzymatic activities, we used a Kruskal-Wallis test (P < 0.05). The results show an increase in the NR activity, as well as in the antioxidant enzymes. The germination time ( t ) and germination rate ( v ) primed in KNO 3 had a better performance compared to the other treatments. In conclusion, the observed benefits in tomato seeds primed with KNO 3 were related to the activity of the enzyme nitrate reductase in the production of nitrite/nitric oxide, which acted promoting a faster germination.

61 citations


Journal ArticleDOI
TL;DR: This review examines the salient issues that relates to the effect of intercropping some selected legume in different cropping patterns with corn in order to improve the yield and forage quality of corn, and that of quality feed/forage production.
Abstract: Low forage quality and low corn yield experienced due to continuous monoculture resulting from persistent soil depletion in the developing world have generated the need for a sustainable practice to improve quality and yield of aforementioned. This review examines the salient issues that relates to the effect of intercropping some selected legume in different cropping patterns with corn in order to improve the yield and forage quality of corn, and that of quality feed/forage production. Two legumes species namely: Bambara groundnut and Peanut were the key crops focused with the main corn crop in this review work.

59 citations


Journal ArticleDOI
TL;DR: The findings of the present study clearly demonstrate that the SPP could be incorporated in fattening lambs diets as an antioxidant, immune-stimulant and growth promoter feed additive.
Abstract: The objective of this study was to investigate the effects of Spirulina platensis powder (SPP) supplementation on growth performance, antioxidative status and blood metabolites in fattening lambs. Ten healthy lambs (46.5 ± 1.06 kg BW) were randomly assigned to one of two treatments (5 lambs per treatment) and received either no supplementation or supplemented with SPP at a rate of 1 g/10 kg BW/day. The feeding experiment was conducted for 35 days with body weight recorded and blood samples collected on days 0, 17 and 35 of the experiment. The paired Student’s t-test for means was used for statistical analysis. The results showed that SPP supplementation improved final live body weight, daily live weight gain, feed intake and feed conversion ratio, compared to the control group (P<0.05). Also, haemoglobin, total white blood cell count, serum globulin, vitamin A and reduced glutathione were higher (P<0.05), while the aspartate amino transferase, alanine amino transferase, cholesterol, glucose and serum malondialdehyde levels were lower (P<0.05) in SPP supplemented group compared with the control. In conclusion, the findings of the present study clearly demonstrate that the SPP could be incorporated in fattening lambs diets as an antioxidant, immune-stimulant and growth promoter feed additive.

Journal ArticleDOI
TL;DR: In this article, the effect of roughage to concentrate ratio (R:C) on rumen pH, fermentation and bacterial population in dairy steers was investigated. And the results showed that high levels of concentrate impacted on volatile fatty acids (VFA) molar proportions and decreased acetate (C2) linearly, while propionate was increased, leading to decreased C2:C3 ratio.
Abstract: The current study was designed to determine the effect of roughage to concentrate ratio (R : C) on rumen pH, fermentation and bacterial population in dairy steers. Four rumen fistulated dairy steers (170±20 kg) were randomly assigned according to a 4×4 Latin square design, in which the steers were fed with four dietary treatments with different R : C ratios of 0·8 : 0·2, 0·6 : 0·4, 0·4 : 0·6 and 0·2 : 0·8, respectively. All animals were kept in individual pens and received feed according to the respective R : C ratios at 0·025 body weight (BW)/d; urea-treated rice straw (prepared using 3·5 kg urea+100 kg water sprayed onto 100 kg of rice straw) was used as a roughage source. The experiment was conducted for four periods of 21 days each. During the first 14 days, feed intake was measured and the animals were then moved to metabolism crates for total urine and faecal collection for 7 days. Total dry matter intake (DMI) was similar among treatments. Energy intake increased as the proportion of concentrate in the diet increased. Apparent digestibilities of dry matter (DM), organic matter (OM) and crude protein (CP) were improved, while neutral detergent fibre (NDF) and acid detergent fibre (ADF) were reduced when the levels of concentrate increased. A decreasing ratio of R : C reduced rumen pH linearly, from 6·4 to 5·9 at 0·2 : 0·8. High levels of concentrate impacted on volatile fatty acids (VFA) molar proportions and decreased acetate (C2) linearly, while propionate (C3) was increased, leading to decreased C2 : C3 ratio. Numbers of protozoa, fungi and proteolytic bacteria were not affected by R : C ratio. Cellulolytic bacteria decreased linearly while amylolytic bacteria increased linearly with 0·60 and 0·80 concentrates. Quantitative polymerase chain reaction (qPCR) based on 16S RNA revealed that Fibrobacter succinogenes numbers were increased when steers were fed with R : C ratio of 0·8 : 0·2. Conjugated linoleic acid (CLA)-producing bacteria, especially those of Butyrivibrio fibrisolvens, increased linearly with R : C ratios of 0·8 : 0·2 and 0·6 : 0·4, while Megasphaera elsdenii, a lactate-utilizing bacterium and reported producer of trans-10, cis-12 CLA increased linearly with R : C ratio of 0·8 : 0·2. In addition, microbial CP synthesis increased quadratically when steers were fed high levels of concentrate. However, the efficiency microbial N synthesis (EMNS) based on OM, truly digested in the rumen, was not affected by different R : C ratios. From the current study, it can be concluded that roughage to concentrate ratio of 0·4 : 0·6 had positive effects for the creation of healthy rumen (rumen pH and ecology), and improved energy intake and rumen fermentation, particularly propionic acid and microbial protein synthesis, in dairy steers fed urea-treated rice straw as a roughage source.

Journal ArticleDOI
TL;DR: In this paper, the effects of AM fungi on wheat N capture after the addition of 15 N-labeled organic matter (OM) to soil was investigated and it was shown that AM fungi increased soil N mineralization rates and total plant N uptake, but they strongly reduced wheat N recovery from decomposing organic matter.
Abstract: Arbuscular mycorrhizal (AM) fungi increase nitrogen (N) uptake by their host plants, but their role in plant N capture from soil organic material is still unclear. In particular, it is not clear if AM fungi compete with the host plant for the N coming from the decomposing organic matter (OM), especially when the AM extraradical mycelium (ERM) and plant roots share the same soil volume. The goal of the present research was to study the effects of AM fungi on wheat N capture after the addition of 15 N-labelled OM to soil. Durum wheat ( Triticum durum ) was grown under controlled conditions in a sand:soil mix and the following treatments were applied: (1) AM inoculation with Glomus mosseae and uninoculated control; and (2) soil amended with 15 N-enriched maize leaves and unamended soil. The addition of OM reduced plant growth and N uptake. The AM fungi increased both plant growth and N uptake compared with uninoculated control plants and the effect was enhanced when wheat was grown in soil amended with OM compared with the unamended control. Although AM fungi increased soil N mineralization rates and total plant N uptake, they strongly reduced wheat N recovery from OM, suggesting that AM fungi have marked effects on competition between plants and bacteria for the different N sources in soil.

Journal ArticleDOI
TL;DR: In this paper, the importance of denitrification within the nitrogen cycle is highlighted and used as an example of the progress achieved in recent times, and possible research pathways are outlined, as well as the methodological advances should go hand in hand with the development of suitable mathematical models addressing the small scale and the full complexity of the many interacting effects.
Abstract: The nitrogen (N) cycle is one of the best studied elemental cycles. However, the N flows and transformations, in particular in aggregated soils, at small scales and in plant–soil systems are not yet fully understood. Analytical and molecular techniques are now available to address N dynamics at small scales. The methodological advances should go hand in hand with the development of suitable mathematical models addressing the small scale and the full complexity of the many interacting effects. The importance of denitrification within the N cycle is highlighted and used as an example of the progress achieved in recent times. Research gaps and possible research pathways are outlined.

Journal ArticleDOI
TL;DR: Genotypes exhibiting traits of high RWC values, high chlorophyll contents and high photosynthetic radiation use efficiency under low moisture availability should be targeted for selection and variety development in programmes aimed at improving sugarcane for drought prone environments.
Abstract: The relationships between physiological variables and sugarcane productivity under water deficit conditions were investigated in field studies during 2005 and 2006 in Weslaco, Texas, USA. A total of 78 genotypes and two commercial varieties were studied, one of which was drought-tolerant (TCP93-4245) and the other drought-sensitive (TCP87-3388). All genotypes were subjected to two irrigation regimes: a control well-watered treatment (wet) and a moderate water-deficit stress (dry) treatment for a period of 90 days. Maximum quantum efficiency of photosystem II (Fv/Fm), estimated chlorophyll content (SPAD index), leaf temperature (LT), leaf relative water content (RWC) and productivity were measured. The productivity of all genotypes was, on average, affected negatively; however, certain genotypes did not suffer significant reduction. Under water deficit, the productivity of the genotypes was positively and significantly correlated with Fv/Fm, SPAD index and RWC, while LT had a negative correlation. These findings suggest that genotypes exhibiting traits of high RWC values, high chlorophyll contents and high photosynthetic radiation use efficiency under low moisture availability should be targeted for selection and variety development in programmes aimed at improving sugarcane for drought prone environments.

Journal ArticleDOI
TL;DR: The positive relationships observed in this study imply that the desirable traits in these hybrids could be exploited in further breeding activities for the development of composites and synthetics for the resource constrained maize farmers who cannot access hybrid seeds every year.
Abstract: A total of fourty two maize hybrids produced through a full diallel mating design and seven parental inbred lines were evaluated in three sites located at different agro-ecological zones in Kenya to determine the genetic parameters governing the inheritance of grain yield and related agronomical traits. The genetic parameters studied among the traits included the mean performance, genotypic variances, phenotypic variances, genotype by environment variances, broad sense heritability and phenotypic and genotypic correlation coefficients. Significant differences were recorded for all traits studied thereby revealing the diversity of the maize genotypes. The grain yield and days to maturity which showed high heritability had low genotypic variances suggesting the involvement of non-additive gene action which could be utilized through heterosis breeding. Ear height and plant height showed the highest heritability and high genotypic variances suggesting the preponderance of additive gene action. Grain yield was positively and strongly correlated with ear height and plant height. The tall plants with high ear placement gave better yields and this could be attributed to the high dry matter accumulation function carried out by the high number of leaves possessed. The positive relationships observed in this study imply that the desirable traits in these hybrids could be exploited in further breeding activities for the development of composites and synthetics for the resource constrained maize farmers who cannot access hybrid seeds every year.

Journal ArticleDOI
TL;DR: Education level of household head, size of household and number of daily meals served significantly influenced decision to access finance while loan payback period, having a phone and personal savings influenced the intensity of participation in the rural financial market.
Abstract: Smallholder farmers in Zambia face many challenges in accessing financial services including limited access to financial markets. Despite the numerous reforms undertaken by the Government and the donor community, including financial sector reforms, many rural farmers have remained in poverty with limited capacity to access safety nets like loans to militate against hunger and disease. This paper set out to find out factors that affect smallholders’ decision to access rural finance and the intensity of their participation in the financial markets. A household survey was conducted in five provinces from which thirteen districts were purposively selected. Employing both purposive and random sampling techniques, a pre-tested questionnaire was administered on 1,326 households. Data was analysed using a double huddle model. Results indicated that education level of household head, size of household and number of daily meals served significantly influenced decision to access finance while loan payback period, having a phone and personal savings influenced the intensity of participation in the rural financial market.

Journal ArticleDOI
TL;DR: In this article, the UK Government's Foresight report on the future of food and farming has been used to set the scene for discussions about the sustainable use of nitrogen in agriculture.
Abstract: The present paper reviews some of the high-level challenges facing the global food system over the next 40 years, drawing in particular on the UK Government's Foresight Report on the ‘Future of Food and Farming’, to set the scene for discussions about the sustainable use of nitrogen in agriculture. It reviews the likely demand and supply pressures on the food system, and the pressing requirements to improve sustainability and address the needs of the world's poorest. It argues that the food system is entering a period of radical change, led particularly by growth in demand, which requires action on food production, diet, waste and efficiency, and governance. A key challenge on the supply side is sustainable intensification – producing more from the same amount of land with fewer and less profound negative effects on the environment. Increasing the efficiency of nitrogen fertilizer application will be critical for increasing yields while reducing the many major environmental consequences of leaching and run-off.

Journal ArticleDOI
TL;DR: A meta-analysis was performed on the results of previous studies from the literature to quantify the benefit of mixed grazing with sheep and cattle and identify determinants and enhancing factors.
Abstract: Mixed grazing is an alternative pasture management which can be used to increase ruminant performance and reduce gastro-intestinal nematodes. A meta-analysis was performed on the results of previous studies from the literature to quantify the benefit of mixed grazing with sheep and cattle and identify determinants and enhancing factors. The analysis focused on papers reporting measurements of average daily weight gain (ADG) of sheep and cattle, carried out simultaneously in mixed grazing and in mono-grazing. The meta-analysis used 179 observations from 16 experiments published in nine carefully selected papers. The benefits of mixed grazing were appraised using the individual ADG of sheep (ADGisheep), cattle (ADGicattle) and the overall performances (ADG per hectare (ADGha), g/day/ha). A theoretical production value (ADGha-Theo) was calculated from the ADGha in mixed and mono-grazing at similar stocking rates (SRs)/ha. The ADGisheep was greater in mixed treatments compared with control treatments (+14·5 g/day, P<0·001) and varied with physiological status and SR (P<0·001). The ADGicattle was similar in mixed and mono-grazing. At a global level ADGha in mixed treatments (ADGha-Mtrt) was higher by 28·6% compared with sheep alone and by 25·1% compared with cattle alone; also, ADGha-Mtrt was 8% higher than ADGha-Theo. The ADGha varied with physiological status and decreased with the proportion of sheep in association (P<0·01). The contribution of ADGha in mixed grazing due to each species was lower compared with gains for each species reared alone (1438 v. 2423 g/day/ha for sheep and 1687 v. 2491 g/day/ha for cattle, P<0·001).

Journal ArticleDOI
TL;DR: The allelic composition at five glutenin loci was assessed by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS–PAGE) on a set of 155 landraces and 18 representative modern varieties to determine gluten strength.
Abstract: The allelic composition at five glutenin loci was assessed by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS–PAGE) on a set of 155 landraces (from 21 Mediterranean countries) and 18 representative modern varieties. Gluten strength was determined by SDS-sedimentation on samples grown under rainfed conditions during 3 years in north-eastern Spain. One hundred and fourteen alleles/banding patterns were identified (25 at Glu-1 and 89 at Glu-2/Glu-3 loci); 0·85 of them were in landraces at very low frequency and 0·72 were unreported. Genetic diversity index was 0·71 for landraces and 0·38 for modern varieties. All modern varieties exhibited medium to strong gluten type with none of their 13 banding patterns having a significant effect on gluten-strength type. Ten banding patterns significantly affected gluten strength in landraces. Alleles Glu-B1e (band 20), Glu-A3a (band 6), Glu-A3d (bands 6 + 11), Glu-B3a (bands 2 + 4+15 + 19) and Glu-B2a (band 12) significantly increased the SDS-value, and their effects were associated with their frequency. Two alleles, Glu-A3b (band 5) and Glu-B2b (null), significantly reduced gluten strength, but only the effect of the latter locus could be associated with its frequency. Only three rare banding patterns affected gluten strength significantly: Glu-B1a (band 7), found in six landraces, had a negative effect, whereas banding patterns 2 + 4+14 + 15 + 18 and 2 + 4+15 + 18 + 19 at Glu-B3 had a positive effect. Landraces with outstanding gluten strength were more frequent in eastern than in western Mediterranean countries. The geographical pattern displayed from the frequencies of Glu-A1c is discussed.

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the relationship between urine N loading rate and the efficacy of a nitrification inhibitor, dicyandiamide (DCD), on cumulative N2O emissions from a grassland soil in Ireland over 80 and 360-day periods in 2009/10 and 2010/11.
Abstract: Nitrous oxide (N2O) emissions associated with urine nitrogen (N) deposition during grazing are a major component of greenhouse gas emissions from domestic livestock The present study investigated the relationship between urine N loading rate and the efficacy of a nitrification inhibitor, dicyandiamide (DCD), on cumulative N2O emissions from a grassland soil in Ireland over 80 and 360-day periods in 2009/10 and 2010/11 A diminishing curvilinear relationship between urine N rate and cumulative N2O emissions was observed in both years Despite this increase in cumulative N2O emissions, the emission factor (EF3) for N2O decreased with increasing urine N rate from, on average, 0·24 to 0·10% (urine applied at 300 and 1000 kg N/ha, respectively), during an 80-day measurement period This was probably the result of a factor other than N, such as carbon (C), limiting the production of N2O The efficacy of DCD varied with urine N loading rate, and inter-annual variability in efficacy was also observed Dicyandiamide was effective at reducing N2O production for 50–80 days after urine application, which accounted for the major period of elevated daily flux However, DCD was ineffective at reducing N2O production after this period, which was likely a result of its removal from the soil via degradation and leaching

Journal ArticleDOI
TL;DR: Citrus essential oils were extraction from hydro distillation technique yielding Citrus oil with reasonable yield as mentioned in this paper. But they did not reveal the properties of the Citrus peel essential oils as food preservatives, phytomedicine and antioxidant agent.
Abstract: Citrus essential oils were extraction from hydro distillation technique yielding Citrus oil with reasonable yield. Phytochemical screening of all five Citrus oils showed that alkaloids, tannins, sterols, terpenoids, saponoins, flavonoids were present (50-80%). GC/MS analysis showed highest percentage of limonene (58-89%) in Citrus oils. Antioxidant study revealed that Citrus peel oils have strong scavenging activity (83%-91%). Antimicrobial activity was evaluated by agar well method against eight common pathogens depicted marked antimicrobial potential especially tangerine (4.9-1.9 cm inhibition zones) and grapefruit oil (4.5-1.2 cm) inhibition zones. The studies emphasized the therapeutic and commercial utilization of Citrus peel essential oils as food preservatives, phytomedicine and antioxidant agent.

Journal ArticleDOI
TL;DR: In this article, the coupling of groundwater and surface water nitrogen dynamics over 3 years, and considered intensive agricultural land-management influences over this period where the risk of N loss to water was considered high.
Abstract: The current study investigated the coupling of groundwater and surface water nitrogen (N) dynamics over 3 years, and considered intensive agricultural land-management influences over this period where the risk of N loss to water was considered high. Groundwater N (as nitrate) was monitored monthly in different strata and zones in four hillslopes, two in each of two agricultural catchments of c. 10 km2, and stream water N flux was monitored sub-hourly in the catchment outlets. Field nutrient sources were connected to surface water via groundwater; the groundwater along hillslopes was seen to be influenced spatially and temporally by management, geology and weather as observed in the concentration variability of nitrate in groundwater. Based on spatio-temporal averages of nitrate-N concentration, groundwater status was considered good (at least below a maximum acceptable concentration (MAC) of 11·3 mg/l). However, zones coincident with land-use change (ploughing and reseeding, typical of a management event in intensive landscapes), showed high spatio-temporal variability in nitrate-N concentration, exceeding the MAC temporarily, before recovering. This spatio-temporal variability highlighted the need for insight into these differences when interpreting groundwater quality data from a limited number of basin-scale sampling points and occasions. In both catchments the 3-year mean nitrate-N concentration in stream water was similar to the spatio-temporal mean concentration in groundwater. The magnitude and variability of loads, however, were more related to changes in annual runoff rather than changes in annual groundwater nitrate-N status. In one wet year, nitrate-N loads exceeded 48 kg/ha from an Arable catchment and 45 kg/ha from a grassland catchment (close to double the loss in a dry year).

Journal ArticleDOI
Zhen Li, Zhang Runhua, X. Wang, Feng Chen1, D. Lai, C. Tian 
TL;DR: In this paper, the effects of a modern cultivation system of plastic film mulching with drip irrigation on soil greenhouse gas fluxes, methane (CH4) and nitrous oxide (N2O) fluxes were quantified and contrasted in an MD system and a traditional system of mulch-free flood-irrigated (MFF) cotton (Gossypium hirsutum L.) in fields of northwest China.
Abstract: To evaluate the effects of a modern cultivation system of plastic film mulching with drip irrigation (MD) on soil greenhouse gas fluxes, methane (CH4) and nitrous oxide (N2O) fluxes were quantified and contrasted in an MD system and a traditional system of mulch-free flood-irrigated (MFF) cotton (Gossypium hirsutum L.) in fields of northwest China. The results showed that soil N2O flux and the absorption rate of CH4 were lower in the MD than the MFF sites. A possible reason for the higher CH4 emissions at MD sites was that the relatively low gaseous oxygen (O2) availability and high ammonium (NH4+) content in the MD soil increased CH4 generation by methanogens and decreased CH4 oxidation by methanotrophs. The lower N2O in the MD sites may be due to an increase of soil denitrification by Thiobacillus denitrificans that reduced some nitrous compounds further into nitrogen gas (N2). Taking into account the global warming potentials of CH4 and N2O in a 100-year time horizon, during the entire growth period, the contribution of CH4 to the greenhouse effect was significantly lower than N2O in these two treatments. Considering these two greenhouse gas fluxes together, a transition from non-mulching cultivation to mulching cultivation could reduce atmospheric emissions by c. 20 g CO2 e m2/season. Based on these findings and previous studies, it can be concluded that mulched-drip irrigation cultivation is a good way to decrease the emission of greenhouse gases and reduce the global warming impact of arid farmlands.

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TL;DR: In this paper, a 3-year (2009-2011) survey was used to assess farm-gate N balances and N use efficiency (NUE) on 21 intensive grass-based dairy farms operating under the good agricultural practice (GAP) regulations in Ireland.
Abstract: There is increasing concern about balancing agronomic and environmental gains from nitrogen (N) usage on dairy farms. Data from a 3-year (2009–2011) survey were used to assess farm-gate N balances and N use efficiency (NUE) on 21 intensive grass-based dairy farms operating under the good agricultural practice (GAP) regulations in Ireland. Mean stocking rate (SR) was 2·06 livestock units (LU)/ha, mean N surplus was 175 kg/ha, or 0·28 kg N/kg milk solids (MS), and mean NUE was 0·23. Nitrogen inputs were dominated by inorganic fertilizer (186 kg N/ha) and concentrates (26·6 kg N/ha), whereas outputs were dominated by milk (40·2 kg N/ha) and livestock (12·8 kg N/ha). Comparison with similar studies carried out before the introduction of the GAP regulations in 2006 would suggest that N surplus, both per ha and per kg MS, have significantly decreased (by 40 and 32%, respectively) and NUE increased (by 27%), mostly due to decreased inorganic fertilizer N input and improvements in N management, with a notable shift towards spring application of organic manures, indicating improved awareness of the fertilizer value of organic manures and good compliance with the GAP regulations regarding fertilizer application timing. These results would suggest a positive impact of the GAP regulations on dairy farm N surplus and NUE, indicating an improvement in both environmental and economic sustainability of dairy production through improved resource-use efficiencies. Such improvements will be necessary to achieve national targets of improved water quality and increased efficiency/sustainability of the dairy industry. The weak impact of SR on N surplus found in the present study would suggest that, with good management, increased SR and milk output per ha may be achievable, while decreasing N surplus per ha. Mean N surplus was lower than the overall mean surplus (224 kg N/ha) from six studies of northern and continental European dairy farms, while mean NUE was similar, largely due to the low input/output system that is more typical in Ireland, with seasonal milk production (compact spring calving), low use of concentrates, imported feed and forages, high use of grazed grass and lower milk yields per ha.

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TL;DR: It is proposed that S. officinalis has the potential to inhibit rumen methanogenesis without affecting rumen fermentation adversely, and the saponin fraction exerted the greatest impact on ruminal fermentation.
Abstract: SUMMARY Although the effect of saponins or saponin-containing plants on rumen microorganisms and rumen fermentation has been intensively investigated, this issue still requires special attention. Many of the phenomena occurring in the rumen related to dietary saponin supplementation are still not fully understood. Saponaria officinalis is a triterpenoid saponin-containing plant; thus, the aim of the present study was to evaluate the effect of S. officinalis L. powdered root, methanolic extract of the S. officinalis root (SOR) and the effect of the separated fractions (polysaccharides, saponins and phenolics) of S. officinalis on rumen methanogenesis, microbial population and rumen fermentation characteristics in an in vitro batch culture fermentation system. The powdered root (raw plant material) and S. officinalis extract (SOE) decreased in vitro methane production and consequently reduced the microbial population in a dose-dependent manner. The inhibition of methanogenesis was accompanied by changes in the volatile fatty acids profile. In vitro dry matter digestibility was not affected by any of the secondary compounds applied. The highest applied doses of SOE caused a higher reduction in methanogenesis (33·5 v. 14·4%) than the highest doses of powdered root form. Such results suggest that the basic components of the SOR could interact with phytochemicals or that the phytochemicals became physically less available for microbiota, resulting in a decreased antimethanogenic activity of the powdered root v. the extract. Among all the fractions selected, the saponin fraction exerted the greatest impact on ruminal fermentation. In conclusion, saponins decreased methane production by 29% in comparison with the control. This decreasewasrelatedto the reduction in protozoa and methanogen counts. It is proposed that S. officinalis has the potential to inhibit rumen methanogenesis without affecting rumen fermentation adversely.

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TL;DR: Land productivity and efficiency were higher with the IF herd than the PF herd due to the higher energy intake per kg feed, but within the given conditions, the more interesting case, economically, might be the reduced costs and improved milk quality of the PF system rather than the increased milk yield of the IF cows.
Abstract: The current situation of volatile milk prices and rising costs of, e.g. grain and labour, suggests that it is worth studying productivity and efficiency in dairy farming. The objective of the current whole-system study, carried out in lowland Central Switzerland from 2007 to 2010, was to compare the performance, efficiency, land productivity and profitability of indoor-feeding (IF) dairy production with that of pasture-based feeding (PF) dairy production. An IF herd consisting of 11 Holstein–Friesian (HF) and 13 Brown Swiss (BS) cows was kept in a free-stall barn and fed a part-mixed ration (PMR) of maize silage, grass silage and protein concentrate. The cows were allocated 15·8 ha of agricultural land (AL). In the PMR, an average per lactation of 443 kg protein concentrate and 651 kg compound feed was fed by a concentrate dispenser according to the requirements of each cow. The PF herd comprised 14 Swiss Fleckvieh (SF) and 14 BS cows, which were kept in a free-stall barn throughout the winter; barn-ventilated hay was offered ad libitum during the lactation period. This herd was allocated 15·7 ha of AL. After calving in spring, the PF cows grazed on semi-continuous pastures; they consumed an average of 285 kg of concentrate per lactation. The IF cows of the BS breed produced significantly more energy-corrected milk (ECM) per standard lactation compared with PF cows (8750 v. 5610 kg), more milk fat (350 v. 213 kg) and more milk protein (306 v. 203 kg). However, the milk of PF cows had higher levels of conjugated linoleic acid (CLA) (1·9 v. 0·6 g/100 g fat) and ω−3 fatty acids (1·7 v. 0·9 g/100 g fat) than the milk of the IF cows. The calving interval (378 v. 405 days) and the empty time (87 v. 118 days) of the BS breed were significantly shorter in the PF in comparison with that of the IF production system. The IF herd yielded significantly higher ECM/ha AL and year (12 716 v. 10 307 kg), and showed a higher feed efficiency (1·3 v. 1·1 kg ECM/kg of total dry matter intake (DMI)). The productivity per hour was roughly similar in the two systems (IF: 76 v. PF: 73 kg milk/h). The PF system resulted in higher labour income compared with the IF system (20·7 v. 13·4 €/h), but the difference was not significant. In conclusion, land productivity and efficiency were higher with the IF herd than the PF herd due to the higher energy intake per kg feed. However, within the given conditions, the more interesting case, economically, might be the reduced costs and improved milk quality of the PF system rather than the increased milk yield of the IF cows.

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TL;DR: The crop growth model HERMES was used to estimate the yields of field crop rotations at a reasonable level, with an index of agreement (IA) ranging from 0·82 to 0·96 for the calibration database.
Abstract: The crop growth model HERMES was used to model crop rotation cycles at 12 experimental sites in the Czech Republic A wide range of crops (spring and winter barley, winter wheat, maize, potatoes, sugar beet, winter rape, oats, alfalfa and grass), cultivated between 1981 and 2009 under various soil and climatic conditions, were included The model was able to estimate the yields of field crop rotations at a reasonable level, with an index of agreement (IA) ranging from 0·82 to 0·96 for the calibration database (the median coefficient of determination (R2) was 0·71), while IA for verification varied from 0·62 to 0·93 (median R2 was 0·78) Grass yields were also estimated at a reasonable level of accuracy The estimates were less accurate for the above-ground biomass at harvest (the medians for IA were 0·76 and 0·72 for calibration and verification, respectively, and analogous medians of R2 were 0·50 and 0·49) The soil mineral nitrogen (N) content under the field crops was simulated with good precision, with the IA ranging from 0·49 to 0·74 for calibration and from 0·43 to 0·68 for verification Generally, the soil mineral N was underestimated, and more accurate results were achieved at locations with intensive fertilization Simulated yields, soil N, water and organic carbon (C) contents were compared with long-term field measurements at Němcice, located within the fertile Moravian lowland At this station, all of the observed parameters were reproduced with a reasonable level of accuracy In the case of the organic C content, HERMES reproduced a decrease ranging from c 85 to 77 tonnes (t)/ha (for the 0–0·3 m soil layer) between the years 1980 and 2007 In spite of its relatively simple approach and restricted input data, HERMES was proven to be robust across various conditions, which is a precondition for its future use for both theoretical and practical purposes

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TL;DR: The practical implication of this finding is that early weeding starting from 3 WAS is very crucial for cowpea production while the critical period of weed removal for optimum yield incowpea is between 3 and 9 WAS in the forest-savannah transitional zone of south Western Nigeria.
Abstract: Weed problem appears to be the most deleterious factor causing between 25 and 60% reduction in potential yield of cowpea. Field trials were therefore conducted to study the effect of inter-row spacing and period of weed interference on growth and yield of cowpea (Vigna unguiculata (L) Walp) at the Teaching and Research Farm of the Federal University of Agriculture, Abeokuta (07° 15'; 03° 25' E) in South Western Nigeria during the early and late wet seasons of 2009. The experiment consisted of eight main plots of weed interference which included initial weed removal for 3, 6, 9, and 12 weeks after sowing (WAS) and subsequently weed –infested until harvest as well as initial weed infestation for corresponding periods and thereafter kept weed free until harvest. There were also sub-plot treatments of three inter-row spacing of 60, 75, and 90 cm. All treatments in different combinations were laid out in a split-plot design with three replications. In both trials, the use of inter-row spacing of 60 cm resulted in significant reduction in weed growth as evident in lower weed dry matter production and subsequent higher cowpea pod and grain yields than those of 75 and 90 cm inter-row spacing. Initial weed infestation of up to 3 WAS did not have any adverse effect on crop growth and cowpea grain yields provided the weeds were subsequently removed. On the other hand, cowpea grain yield loss was not significantly averted by keeping the crop weed free for only 3 WAS without subsequent weed removal. In this study, initial weed-infestation for 6 WAS and beyond significantly depressed various crop growth parameter and cowpea grain yield compared with the crop kept weed free throughout its life cycle. In order to obtain optimum yields similar to that of the weed free cowpea field, it was required to keep the crop weed free for 6 WAS and beyond. However, frequent weeding beyond 9 weeks after sowing did not improve cowpea yield significantly and as a matter of fact it may even result in reduction of cowpea grain yield due to mechanical damage of hoe weeding. The practical implication of this finding is that early weeding starting from 3 WAS is very crucial for cowpea production while the critical period of weed removal for optimum yield in cowpea is between 3 and 9 WAS in the forest-savannah transitional zone of south Western Nigeria.

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TL;DR: The findings indicated the presence of ALS in Uganda with high incidence, severity and variability across districts and altitudes, and strategic interventions involving integrated disease management and breeding for durable resistance are required to manage the disease.
Abstract: Pseudocercospora griseola the casual pathogen for angular leaf spot (ALS) on common bean (Phaseolus vulgaris) is highly variable with many different races occurring in different agro-ecological zones. Therefore, for an effective breeding program, there is a need to continuously monitor the distribution and variability of the pathogen as a means of guiding breeders on which races to target. A study was conducted to assess the incidence, severity and variability of P. griseola in bean growing districts of Dokolo, Lira, Apac, Sironko, Mbale, Rakai, Lwengo, Bukomansibi, Kabale and Kisoro in Uganda. Forty five out of 80 isolates collected were tested on a set of 12 international bean differentials cultivars for ALS. Molecular markers were also used to elucidate the variability among isolates. In all districts, ALS was found wide spread with a mean incidence of 49.9%. Dokolo district had the highest disease incidence and severity, while Kisoro registered the lowest values respectively. In terms of altitude, incidence (60%) and severity (45%) were high for bean fields located between 1000-1200 m and low disease incidence (33.6%) and severity (20.7%) for fields lying above 1500 m. The standard differential cultivars and molecular markers defined 12 pathotypes and 30 haplotypes respectively. These belonged to Middle America and Andean group with considerably high level of diversity. Our findings indicated the presence of ALS in Uganda with high incidence, severity and variability across districts and altitudes. Hence strategic interventions involving integrated disease management and breeding for durable resistance are required to manage the disease.