Showing papers in "Virus Research in 1986"

TL;DR: Nucleotide sequencing of the cleavage region of the So gene of IBV-M41 revealed the same connecting peptide as IBv-Beaudette and that the first 20 N-terminal residues of S2 of IBM41 were identical to those of the Beaudette strain.

TL;DR: It is demonstrated that AcNPV can be used to express plasma membrane-bound virus glycoproteins and a 65 kDa protein was identified in infected cells by immunoprecipitation.

TL;DR: The structural genome characteristics of BHV-1.3 compared to those of the other BHv-1 strains, examined by means of restriction site mapping, electron microscopy and cross-hybridization are described.

TL;DR: Regulation of transcription within four well-defined regions of the DNA genome of the baculovirus Autographa californica nuclear polyhedrosis virus was studied in a permissive lepidopteran cell line and late RNAs defined by their sensitivity to aphidicolin overlapped the immediate early RNAs.

TL;DR: The nucleotide sequence of cloned cDNA copies of the mRNA encoding the Newcastle disease virus fusion protein was determined and the positions of the cysteine residues within the sequence are conserved, suggesting the importance of disulfide bond formation in the conformation of paramyxovirus fusion proteins.

TL;DR: The human respiratory coronavirus OC43 was grown on a human rectal tumor cell line and was isotopically labeled with amino acids, glucosamine, and orthophosphate to analyze virion structural proteins to resolve four major protein species resolved by electrophoresis.

TL;DR: The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signah peptide and a transmembrane anchor α-helix at the C-terminus in the peplomer protein of avian infectious bronchitis virus.

TL;DR: The concomitant appearance of comparable levels of low molecular weight immune complexes suggests that most anti-LDV IgG was complexed with LDV proteins, whereas antibodies that neutralize the infectivity of exogenously added LDV appeared only 1-2 months p.i.

TL;DR: The patterns of fragments generated by digestion with Hind III are sufficiently similar to show that all capripoxviruses are closely related, although patterns of different isolates can be grouped in a way which correlates with the animal of origin.

TL;DR: A comparison of antigenicity among 15 human strains with monoclonal antibodies obtained in radioimmunoprecipitation and HI tests showed that the regions recognized by the group A antibodies undergo considerable changes, whereas those by group B are conserved among the strains.

TL;DR: Serial undiluted passages of infectious bursal disease virus in chick embryo cells were accompanied by a von Magnus type fluctuation of infectivity in viral harvests and a gradual decrease of plaque size.

TL;DR: Results indicate that the cell-free system supported rotavirus RNA replication, transcription and the assembly of subviral particles.

TL;DR: Anti-C antibodies did not inhibit viral RNA synthesis when added to an extract of infected cells, consistent with the conclusion that the C proteins have no direct role in viral transcription, since virions lack C proteins but are transcriptionally active.

TL;DR: The kinetics and the cytosine arabinoside sensitivity of the synthesis of the virus-induced polypeptides showed the existence of three classes of proteins, two early and one late, and protein p220 was incorporated into the membrane very soon after its synthesis, before the appearance of intracellular infectious virus.

TL;DR: Peptide map analysis shows that this in vivo product has the same primary sequence as the NS-1 protein previously identified in the in vitro translation products of MVM encoded mRNA, and is the product of the largest messenger RNA species, a spliced 4.8 kb (R1) transcript.

TL;DR: A 102 base cDNA copy of the 5' leader sequence of the Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin mRNA was made using the technique of primer extension with reverse transcriptase, suggesting that the processing of the 3' end of the mRNA occurs in a manner similar to that observed in other virus and cellular systems.

TL;DR: The recombinant designated R-M1-C1 was highly pathogenic for the tree shrew, but slightly virulent for inbred mouse strain A, suggesting that the viral DNA sequence involved in the i.p. pathogenicity of HSV-1 is located within the genome coordinates 0.761-0.796.

TL;DR: The BHV-4-induced thymidine kinase (TK) differed from the principal cytosol TK of mock-infected cells in PAGE mobility under non-denaturing conditions and in the capacity to efficiently substitute CTP for ATP as a phosphate donor.

TL;DR: Each overlapping set of RNAs exhibited unique characteristics including variations in the number of overlapping transcripts, their temporal regulation, and their relative abundance during the course of infection.

TL;DR: To identify the specific sequences present in each of the polycistronic RNA species, cDNA clones generated by reverse transcription of NDV mRNAs were characterized and used as probes on Northern blots of total NDV cytoplasmic RNA.

TL;DR: The assumption that monoclonal antibodies, which failed to inhibit hemagglutination of the virus yet neutralized viral infectivity, inhibited the fusion step in the viral replication process by interfering with a low pH-induced conformational change in the HA molecule is supported.

TL;DR: In cells infected with wild-type virus for 4 h or longer, HSV proved to be a more potent mitotic arresting agent than colcemid, and studies with selected mutants indicate that HSV pol specifically may be involved in mitotic arrest.

TL;DR: It is suggested that negative strand RNA is synthesized during the early period of the infectious cycle and is stable in infected cells and also suggest that treatment with cycloheximide at late times does not inhibit positive strand RNA synthesis indirectly by blocking the formation of negative strand templates.

TL;DR: It was found that the accumulation of p14 and two related proteins is due to an active response of the tomato plant to infection and not caused by the degradation of a preexisting leaf protein.

TL;DR: It was concluded that S2 is very sensitive to hydrolysis near its carboxy terminus at residues close to the outer membrane surface in avian infectious bronchitis virus and its purified protein components and virions of IBV-Beaudette.

TL;DR: Three different types of influenza C virus were isolated during the period of 12 mth from March 1981 to February 1982, suggesting that multiple influenza C viruses with distant genetic relationship were circulating at the same time in Japan.

TL;DR: Monensin inhibits the intracellular transport of the glycoproteins of Newcastle disease virus between cis and trans Golgi stacks of infected BHK cells, as evidenced by its effect upon their post-translational modifications such as fatty acid acylation, glycosylation and proteolytic cleavage.

TL;DR: The results suggest that ADP-ribosylation of adenovirus core proteins may have a role in virus decapsidation.

TL;DR: It was found that extra methyl groups on the caps cause a lower rate of initiation in the late 26S Semliki Forest viral mRNA, and the cap analog m2,2,7Gp was slightly less inhibitory than m 7Gp in an in vitro translation system.

TL;DR: The results confirm those of Abraham and Pattnaik (1983) that ongoing protein synthesis is required for the accumulation of complete bunyavirus S-mRNA.