Journal ArticleDOI
3β-Hydroxysteroid dehydrogenase/Δ5→4-isomerase expression in rat and characterization of the testis isoform
M C Lorence,Danielle Naville,Sandra E. Graham-Lorence,Serdia O. Mack,Barbara A. Murry,John M. Trant,J. Ian Mason +6 more
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TLDR
While one gene product is highly expressed in testicular Leydig cells, and probably adrenal and ovary, accounting for their 3β-HSD content, a 3β,HSD is also expressed in liver in a sex-specific manner.About:
This article is published in Molecular and Cellular Endocrinology.The article was published on 1991-09-01. It has received 51 citations till now. The article focuses on the topics: Complementary DNA & Pregnenolone.read more
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A key enzyme in the biosynthesis of neurosteroids, 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase (3β-HSD), is expressed in rat brain
TL;DR: In this article, the selective expression of a 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD) isoform in rat brain was reported.
Journal ArticleDOI
Protein kinase B is obligatory for follicle-stimulating hormone-induced granulosa cell differentiation.
TL;DR: It is demonstrated that PKB is an essential component of the FSH-mediated granulosa cell differentiation and that both PKB and G(s)alpha signaling pathways are required.
Journal ArticleDOI
Widespread Capacity for Steroid Synthesis in the Avian Brain and Song System
TL;DR: It is demonstrated that the steroidogenic acute regulatory protein (StAR), cytochrome P450 side-chain cleavage (CYP11A1), and 3beta-hydroxysteroid dehydrogenase/Delta5-Delta4 isomerase, the first three factors in the steroidogenesis pathway, are expressed in both developing and adult zebra finch brain.
Journal ArticleDOI
Structure, function and tissue-specific gene expression of 3β-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase enzymes in classical and peripheral intracrine steroidogenic tissues.
TL;DR: It is demonstrated that the lower activity of expressed rat type II compared to rat type I 3β-HSD is due to a change of four residues probably involved in a membrane-spanning domain, indicating an intrinsic androgenic 17β-hydroxysteroid dehydrogenase (17β- HSD) activity.
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The acute and chronic effects of adrenocorticotropin on the levels of messenger ribonucleic acid and protein of steroidogenic enzymes in rat adrenal in vivo.
TL;DR: The effects of acute and chronic stimulation by ACTH on changes occurring in the temporal expression of steroidogenic enzymes in the rat adrenal in vivo confirm the importance of physiological concentrations of ACTH in maintaining normal levels of adrenocortical enzymes and indicate that in addition to ACTH, other factors are involved in controlling the expression of StAR protein.
References
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.