Showing papers in "The Journal of Steroid Biochemistry and Molecular Biology in 1992"

TL;DR: A simple-to-perform synthesis yielded a conjugate suitable for use as a tracer in immunoassays for cortisol measurement and showed excellent correlation with a commercially available radioimmunoassay adapted for salivary cortisol measurement.

TL;DR: The final part of the review on regulation of steroidogenesis includes a description of the normal physiological fluctuations in the steroid output of adrenal cortex and gonads, and provides an analysis of the relative role of enzyme levels in the determination of these fluctuations.

TL;DR: The mutated androgen receptor of LNCaP cells is a useful tool in the elucidation of different levels of action of steroids and antisteroids and the mutation directly affects both binding specificity and the induction of gene expression.

TL;DR: It is suggested that lignans and isoflavonoids may affect uptake and metabolism of sex hormones by participating in the regulation of plasma SHBG levels and in this way influence their biological activity and that they may inhibit cancer cell growth like some flavonoids by competing with estradiol for the type II estrogen binding sites.

TL;DR: ICI 182,780 provides the opportunity to evaluate clinically the potential therapeutic benefits of complete blockade of oestrogen effects in endocrine-responsive human breast cancer.

TL;DR: The clinical implications of the presence of SS-R in tumors are manyfold: as a predictive marker for efficient therapy with octreotide in pituitary and GEP tumors; as a diagnostic marker for pathobiochemical classification of tumors, using in vitro detection methods; for clinical evaluation using in vivo scanning techniques; and as a prognostic marker.

TL;DR: It is concluded that Enl inhibits E2-stimulated MCF-7 breast cancer cell growth in vitro, and vice versa, and the concentrations of Enl needed for the elimination of the proliferative effect of E2 are physiologic and similar to those used in corresponding experiments utilizing tamoxifen.

TL;DR: Evidence is presented that the receptor of the genomic effects is likely to be separate from the receptor/membrane recognition element which initiates the rapid nongenomic biological effects of this steroid hormone.

TL;DR: The widespread distribution of 17β-HSD in rat and human tissues clearly indicates the importance of this enzyme in peripheral sex steroid formation or intracrinology.

TL;DR: Findings suggest that murine T cells contain a specific DHEA receptor, a steroid hormone that is directly involved in the regulation of IL-2 production by both normal and some T-cell hybridomas.

TL;DR: Estrogen-induction of PR was more dramatic in glial cells derived from female offsprings than from males, as measured by binding studies and by immunohistochemical techniques with the KC 146 anti-PR monoclonal antibody.

TL;DR: It is suggested that hormonal activation, through these factors, alters membrane structure around mitochondrial intermembrane contact sites, which also function to transfer ADP, phospholipids, and proteins to the inner mitochondria.

TL;DR: It is demonstrated that the lower activity of expressed rat type II compared to rat type I 3β-HSD is due to a change of four residues probably involved in a membrane-spanning domain, indicating an intrinsic androgenic 17β-hydroxysteroid dehydrogenase (17β- HSD) activity.

TL;DR: In another series of similar experiments, changes in 24K, desmin and PP12 expression were obtained by adding to the insulin-containing medium PRL instead of the hormonal mixture, a finding suggesting sequential steps during the decidualization process.

TL;DR: It is still not clear whether anabolic steroids are able to improve athletic performances as well as whether these compounds are active in the presence of normal circulating levels of testosterone.

TL;DR: The possibility of multiple dehydrogenase species in mineralocorticoid target tissues is consistent with the hypothesis that the ubiquitous 'native' 1.4 kB hepatic enzyme regulates the GR, and these separate dehydrogenases regulate the MR.

TL;DR: Ligand-mapping has been used to identify the site of interaction of a steroid substitution within the hormone-binding domain of the receptor and revealed that a single amino acid is responsible for the differential antagonistic effect of RU486 in man, chicken and hamster.

TL;DR: It is suggested that soy as a dietary source plays a protective role against the development of prostatitis in rats, and indicates that the ventral, lateral and dorsal lobes of the rat prostate have different sensitivities to alterations in dietary factors.

TL;DR: Results indicate that, in differentiating gonads of turtle embryos, temperature acts on the regulation of synthesis (and therefore activity) of cytochrome P-450 aromatase (P-450-aro).

TL;DR: The control ofEstradiol concentration can be carried out in the breast cancer tissue itself; estrone sulfate can play an important role in the bioavailability of estradiol in the Breast cancer cell; and the control of: the estrogen sulfatase, estrogen sulfotransferase, and 17 beta-hydroxysteroid dehydrogenase can be new targets for therapeutic applications in breast cancer.

TL;DR: The in vitro data suggest an explanation for the discrepant compounds, consistent with published data on their metabolic fate in vivo, and have utility as a quantitative predictor of in vivo glucocorticoid pharmacology.

TL;DR: It is found that the rat type III mRNA species was below the detection limit in intact female liver while, following hypophysectomy, its accumulation increased to 55% of the levels measured in intact or HYPOX male rats, an increase which can be blocked by administration of ovine prolactin (oPRL).

TL;DR: An expression system that utilized yeast copper metallothionein promoter and ubiquitin fusion technology to express the human estrogen receptor gene in yeast is described and it is demonstrated that tamoxifen and ICI 164,384 are capable of binding to the receptor but neither antiestrogen was able to block the estradiol mediated increase in transcription.

TL;DR: It was indicated that a small amount of cytochrome b5 activated the hydroxylase activity by supplying additional second electrons to oxygenated P-450C21 in the liposomes while a large amount of b5 might suppress the activity through the interferences in the interaction between the reductase and P- 450C21.

TL;DR: Glucocorticoid (GC) excess (Cushing's syndrome) is associated with hypertension in at least 70% of patients, independently of the subtype (pituitary or adrenal) and the duration, but not of the age of the patients.

TL;DR: In the first phase I trial, healthy postmenopausal volunteers were given single oral doses of exemestane, and plasma levels of cortisol, aldosterone, 17-hydroxyprogesterone, DHEAS, LH and FSH were measured up to 5-8 days, and no significant adverse events were observed.

TL;DR: A series of human androgen receptor (AR) deletion mutants was constructed to study the relationship between various structural domains and their different functions in the AR protein, indicating that in the absence of hormone this domain displays an inhibitory function.

TL;DR: Thyroid hormone is a second major regulator of liver steroid hydroxylase P450 activity, and regulates these enzymes directly, at a pretranslational step, and indirectly, through its stimulation of pituitary GH secretion and by its positive effects on the expression of the flavoenzyme NADPH-P450 reductase.

TL;DR: A number of cytochrome P450-dependent enzymes are major targets for both steroidal and nonsteroidal compounds that may be of use in the treatment of a number of androgen-independent, androgen-, estrogen- and other steroid-dependent diseases.

TL;DR: It is found that the more potent inhibitor, 4-hydroxyandrostenedione (4-OHA), which can suppress oestrogen synthesis in rodents and non-human primates with intact ovarian function, was also unsuccessful as an oestrogens suppressant in premenopausal women at its maximum tolerated dose.