A beta-D-galactosidase from nasturtium (Tropaeolum majus L.) cotyledons. Purification, properties, and demonstration that xyloglucan is the natural substrate.
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It is argued that the cell-wall storage xyloglucan of the nasturtium seed is its natural substrate.About:
This article is published in Journal of Biological Chemistry.The article was published on 1988-03-25 and is currently open access. It has received 107 citations till now. The article focuses on the topics: Xyloglucan & Tropaeolum majus.read more
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Journal ArticleDOI
A xyloglucan-oligosaccharide-specific α-d-xylosidase or exo-oligoxyloglucan-α-xylohydrolase from germinated nasturtium (Tropaeolum majus L.) seeds : Purification, properties and its interaction with a xyloglucan-specific eneto-(1→4)-β-d-glucanase and other hydrolases during storage-xyloglucan mobilisation.
TL;DR: The α-xylosidase which is involved in the postgerminative mobilisation of xyloglucan in nasturtium seed cotyledons has now been purified to apparent homogeneity by a facile procedure involving lectin affinity chromatography as mentioned in this paper.
Journal ArticleDOI
Xyloglucan mobilisation in cotyledons of developing plantlets of Hymenaea courbaril L. (Leguminosae-Caesalpinoideae)
TL;DR: A tentative model for xyloglucan disassembling in vivo is proposed, where beta-galactosidase allows the free oligosaccharides to bypass a transglycosylation cycle and be disassembled by the other exo-enzymes.
Journal ArticleDOI
Purification of a β-galactosidase from cotyledons of Hymenaea courbaril L. (Leguminosae). Enzyme properties and biological function
P.H.N. de Alcântara,L. Martim,Clóvis Oliveira Silva,Sonia M. C. Dietrich,Marcos Silveira Buckeridge +4 more
TL;DR: The biochemical properties of hcbetagal as a whole and its high specificity towards XGOs, the low pH optimum and the fact that it is inhibited by its product (galactose) suggest that hc Betagal might be one of the biochemical control points in xyloglucan catabolism in vivo.
Journal ArticleDOI
Microarray analysis of developing flax hypocotyls identifies novel transcripts correlated with specific stages of phloem fibre differentiation.
TL;DR: Temporal and spatial regulation of beta-galactosidase activity suggests a role for this enzyme in remodelling of flax bast fibre cell walls during secondary cell wall deposition.
Journal ArticleDOI
The Control of Storage Xyloglucan Mobilization in Cotyledons of Hymenaea courbaril
TL;DR: It is shown that xyloglucan mobilization is strictly controlled by the development of first leaves of the seedling, with the start of its degradation occurring after the beginning of eophyll (first leaves) expansion.
References
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Journal ArticleDOI
Structure and function of the primary cell walls of plants.
TL;DR: All glycosyl residues (except the residue at the reducing end of an oligosaccharide, which is called a glycose residue) are glycosidically linked at C-l and this fact is assumed in the notation used, and, thus, C-\ is not mentioned.
Journal ArticleDOI
Inhibition of 2,4-dichlorophenoxyacetic Acid-stimulated elongation of pea stem segments by a xyloglucan oligosaccharide.
TL;DR: Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-beta-1,4-glucanase purified from the culture fluid of Trichoderma viride to inhibit the elongation of etiolated pea stem segments.
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Relationship between Promotion of Xyloglucan Metabolism and Induction of Elongation by Indoleacetic Acid
John M. Labavitch,Peter M. Ray +1 more
TL;DR: The results suggest that the changes in xyloglucan reflect the means by which auxin modifies the cell wall to cause elongation.
Book ChapterDOI
Cell Wall Storage Carbohydrates in Seeds—Biochemistry of the Seed “Gums” and “Hemicelluloses”
TL;DR: This chapter outlines the structures and occurrence of cell wall storage carbohydrates to give an account of current research on their metabolism and to explore their overall biological significance in the seeds which contain them.
Journal ArticleDOI
Turnover of cell wall polysaccharides in elongating pea stem segments.
John M. Labavitch,Peter M. Ray +1 more
TL;DR: A highly selective pattern of wall turnover processes with an even more specific influence of auxin is indicated, suggesting that indoleacetic acid induces conversion of wall xyloglucan from insoluble to water-soluble form.