Journal ArticleDOI
A cell line (HBL-100) established from human breast milk
TLDR
HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.Abstract:
A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.read more
Citations
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Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells.
TL;DR: It is shown that a reconstituted basement membrane (BM) can be used to culture all normal human breast epithelial cells and a subset of human breast carcinoma cells and it is proposed that the ability to sense BM appropriately and to form three-dimensional organotypic structures may be the function of a class of "suppressor" genes that are lost as cells become malignant.
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CD44 + /CD24 - breast cancer cells exhibit enhanced invasive properties: an early step necessary for metastasis
Carol Sheridan,Hiromitsu Kishimoto,Robyn K. Fuchs,Sanjana Mehrotra,Poornima Bhat-Nakshatri,Charles H. Turner,Robert J. Goulet,Sunil Badve,Harikrishna Nakshatri +8 more
TL;DR: Breast cancer cells with CD44+/CD24- subpopulation express higher levels of proinvasive genes and have highly invasive properties, however, this phenotype is not sufficient to predict capacity for pulmonary metastasis.
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Relevance of breast cancer cell lines as models for breast tumours: an update
Marc Lacroix,Guy Leclercq +1 more
TL;DR: It is concluded that BCC lines are likely to reflect the features of cancer cells in vivo, and the importance of oestrogen receptor-alpha and Her-2/neu as classifiers for cell lines and tumours is underlined.
Journal ArticleDOI
Triple negative breast cancer cell lines: One tool in the search for better treatment of triple negative breast cancer
TL;DR: Given the lack of validated molecular targets and the poor outcome in patients with TNBC, there is a clear need for a greater understanding of TNBC at all levels and for the development of better therapies.
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Re: Leptin—A Growth Factor in Normal and Malignant Breast Cells and for Normal Mammary Gland Development
Elisabeth Oelmann,Susan Haghgu,Emma Kulimova,Hubert Serve,Christiane Schmitmann,Wolfgang E. Berdel +5 more
TL;DR: Leptin appears to be able to control the proliferation of both normal and malignant breast epithelial cells, and the leptin pathway should be further studied as a target for interventions to treat or prevent breast cancer.
References
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A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
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A simplified lead citrate stain for use in electron microscopy.
TL;DR: This communication reports the use of a commercially available lead citratO to eliminate the lead citrate stain in electron microscopy.
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A Human Cell Line From a Pleural Effusion Derived From a Breast Carcinoma
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Agar suspension culture for the selective assay of cells transformed by polyoma virus.
Ian Macpherson,Luc Montagnier +1 more