Journal ArticleDOI
A fluorimetric method for continuously assaying ATPase: application to small specimens of glycerol-extracted muscle fibers.
Reiji Takashi,Susan Putnam +1 more
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TLDR
A continuous fluorimetric method using auxiliary-coupling enzymes such as pyruvate kinase and lactate dehydrogenase for measuring ADP production to assay ATPase activity is described, which is simpler, more rapid, and more sensitive than the previously used spectrophotometric method.About:
This article is published in Analytical Biochemistry.The article was published on 1979-01-15. It has received 55 citations till now. The article focuses on the topics: ATPase & Pyruvate kinase.read more
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Relaxation of rabbit psoas muscle fibres from rigor by photochemical generation of adenosine-5'-triphosphate.
TL;DR: The results indicate that ATP binding and cross‐bridge detachment from the nucleotide‐free intermediate of thecross‐bridge cycle are rapid compared to the cross‐ bridge cycling rate.
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Muscle performance, morphology and metabolic capacity during strength training and detraining: a one leg model.
TL;DR: The significant alterations in peak torque outputs with both legs suggest that neural adaptations play a prominent role in strength performance with training and detraining.
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An assay for sarcoplasmic reticulum Ca2+-ATPase activity in muscle homogenates
TL;DR: A spectrophotometric method is described for the determination of sarcoplasmic reticulum (SR) Ca2(+)-ATPase activity (EC 3.1.6.38) in unfractionated muscle homogenates, which shows a good correlation over a wide range between SR specific Ca2 (+)-uptake and -ATPases activities.
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Kinetics of the actomyosin ATPase in muscle fibers.
TL;DR: Many characteristics expected from the cyclic ATPase mechanism of Scheme 1 are apparent in reactions measured directly in muscle fibers, suggesting that the mechanical driving stroke of the cross-bridge cycle and events during physiological relaxation are closely linked to the product release steps.
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Activation of fast skeletal muscle: contributions of studies on skinned fibers.
TL;DR: Questions concern the mechanism of the signal that bridges the junctional gap between the two membrane systems, the mechanism and properties of the activated Ca efflux to the myofilament space, and the operation of the Ca pump of the SR during activation.
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Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
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Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Sidney Udenfriend,Stanley Stein,Peter Böhlen,Wallace Dairman,Willy Leimgruber,Manfred Weigele +5 more
TL;DR: Fluorescamine is a new reagent for the detection of primary amines in the picomole range that is almost instantaneous at room temperature in aqueous media and the products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent.
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Mechanism of adenosine triphosphate hydrolysis by actomyosin.
R. W. Lymn,E. W. Taylor +1 more
TL;DR: Analysis of the steady-state kinetics by Eisenberg and Moos showed that the behavior could be interpreted as a decrease in the HMM-actin association constant when ATP is bound and a large increase in actin-HMM ATPase relative to myosin.