Journal ArticleDOI
A genetic study of x-ray sensitive mutants in yeast.
J.C. Game,Robert K. Mortimer +1 more
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Examination of the survival responses of multiple-mutant strains indicated a minimum of two pathways in the repair of X-ray damage, and a number of the mutants have been mapped and these were found to be dispersed over the genome.Abstract:
A set of 64 mutants of Saccharomyces cerevisiae that confer sensitivity to X-ray inactivation were analyzed genetically to determine the number of genetic loci involved. The mode of interaction of various combinations of mutants was also determined. A minimum of 17 genes, when mutant, increase X-ray sensitivity of yeast, primarily by eliminating the resistance of budding haploid cells and by removing the shoulder on the survival curves of diploid cells. Eight mutant loci affect principally X-ray sensitivity while the remaining genes also control sensitivity to ultraviolet. Some of the genes when homozygous block sporulation or result in partial or complete sterility. Examination of the survival responses of multiple-mutant strains indicated a minimum of two pathways in the repair of X-ray damage. A number of the mutants have been mapped and these were found to be dispersed over the genome.read more
Citations
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Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more
TL;DR: This review encompasses different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
Journal ArticleDOI
A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.
Tanya T. Paull,Emmy P. Rogakou,Vikky Yamazaki,Cordula U. Kirchgessner,Martin Gellert,William M. Bonner +5 more
TL;DR: The evidence presented strongly supports a role for the gamma-H2AX and the PI-3 protein kinase family in focus formation at sites of double-strand breaks and suggests the possibility of a change in chromatin structure accompanying double-Strand break repair.
Journal ArticleDOI
Mechanism of eukaryotic homologous recombination.
TL;DR: HR accessory factors that facilitate other stages of the Rad51- and Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.
Journal ArticleDOI
Homologous recombination and non‐homologous end‐joining pathways of DNA double‐strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells
Minoru Takata,Masao S. Sasaki,Eiichiro Sonoda,Ciaran G. Morrison,Mitsumasa Hashimoto,Hiroshi Utsumi,Yuko Yamaguchi-Iwai,Akira Shinohara,Shunichi Takeda +8 more
TL;DR: Observations provide the first genetic evidence that both repair pathways play a role in maintaining chromosomal DNA during the cell cycle.
Journal ArticleDOI
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
TL;DR: It is suggested that the Rad51 protein, probably together with Rad52 protein, is involved in a step to convert DSBs to the next intermediate in recombination.
References
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Journal ArticleDOI
Mutants of yeast defective in mutation induced by ultraviolet light.
TL;DR: To identify new genes controlling UV-induced mutation, it is desirable to select strains directly for defective mutation induction, thereby avoiding the prior condition that all such mutants be UV-sensitive.
Journal ArticleDOI
Genetic control of the cell division cycle in yeast: II. Genes controlling DNA replication and its initiation☆☆☆
TL;DR: Temperature-sensitive mutations occurring in two unlinked complementation groups, cdc4 and cdc8, are recessive and result in a defect in DNA replication at the restrictive temperature.
Journal ArticleDOI
The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast
B.S. Cox,J.M. Parry +1 more
TL;DR: 96 mutants of yeast have been isolated which were more sensitive than wild-type to ultraviolet irradiation, and genetic analysis showed that many are inherited as single mendelian recessive mutations, and that they occupy at lest 22 different loci.
Journal ArticleDOI
Mutants Affecting Meiosis in Natural Populations of DROSOPHILA MELANOGASTER
Journal ArticleDOI
Radiobiological and genetic studies on a polyploid series (haploid to hexaploid) of saccharomyces cerevisiae
TL;DR: The results of the radiation studies are consistent with the assumption that the majority of inactivation of haploid cultures is brought about by recessive lethals, whereas higher-ploidy cultures are inactivated mostly by dominant lethals.