Journal ArticleDOI
A highly sensitive high-performance liquid chromatography method for the estimation of ascorbic and dehydroascorbic acid in tissues, biological fluids, and foods.
Willy A. Behrens,René Madère +1 more
TLDR
The method described here used a specially designed mobile phase, gave greater stability and a noiseless baseline, and increased substantially the sensitivity and precision.About:
This article is published in Analytical Biochemistry.The article was published on 1987-08-15. It has received 199 citations till now. The article focuses on the topics: Ascorbic acid & Dehydroascorbic acid.read more
Citations
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Journal ArticleDOI
Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species
Ronald L. Prior,Guohua Cao,A. Martin,Emin Sofic,J. Mcewen,C. O'brien,N. Lischner,M. Ehlenfeldt,Wilhelmina Kalt,G. Krewer,C. M. Mainland +10 more
TL;DR: Different cultivars of four Vaccinium species were analyzed for total phenolics, total anthocyanins, and antioxidant capacity (oxygen radical absorbance capacity, ORAC).
Journal ArticleDOI
Plant L‐ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing
Mark W. Davey,M. Van Montagu,Dirk Inzé,Maite Sanmartín,Angelos K. Kanellis,Nicholas Smirnoff,I. J. J. Benzie,John (Sean) J. Strain,D. Favell,J. Fletcher +9 more
TL;DR: The role of L-AA in metabolism and the latest studies regarding its bio- synthesis, tissue compartmentalisation, turnover and catabolism are focused on, as well as the potential to improve the L- AA content of crops.
Journal ArticleDOI
Comparison of different analytical methods for assessing total antioxidant capacity of human serum
TL;DR: Three assays were compared for the determination of total antioxidant capacity in human serum: the oxygen radical absorbance capacity (ORAC), the Randox Trolox-equivalent antioxidant capacity (Randox-TEAC) assay, and the ferric reducing ability (FRAP) assay.
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Free and albumin-bound bilirubin are efficient co-antioxidants for alpha-tocopherol, inhibiting plasma and low density lipoprotein lipid peroxidation.
Jiri Neuzil,Roland Stocker +1 more
TL;DR: Results show that bilirubin inhibits oxidation of LDL lipids initiated within the lipoprotein core and indicate that this activity is mediated by interaction of the pigment with LDL's alpha-tocopherol.
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Serum Antioxidant Capacity Is Increased by Consumption of Strawberries, Spinach, Red Wine or Vitamin C in Elderly Women
TL;DR: It is concluded that the consumption of strawberries, spinach or red wine, which are rich in antioxidant phenolic compounds, can increase the serum antioxidant capacity in humans.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
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Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
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Synthesis and some major functions of vitamin C in animals.
TL;DR: Using the in vitro method, the ascorbic acid synthesizing abilities of different species of animals in the phylogenetic tree are examined, and the results are given below.
Journal ArticleDOI
The use of homocysteine in the estimation of dehydroascorbic acid.
TL;DR: An account is given of a new method, based on the two findings that homocysteine rapidly reduces dehydroascorbic acid at pH 7 0, and that under certain simple conditions 2:6-dichlorophenolindophenol can be used to estimate ascorbic acid in the presence of homocy steine without interference from the latter compound.
Journal ArticleDOI
Ascorbic acid in lymphocytes: cell preparation and liquid-chromatographic assay.
TL;DR: Measurements of ascorbic acid concentration in leukocytes by "high-performance" liquid chromatography (HPLC) provides better nutritional assessment, leading to better management, particularly of presymptomatic and critically ill patients.