A Membrane-Bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis
Sophia Ng,Aneta Ivanova,Owen Duncan,Simon R. Law,Olivier Van Aken,Inge De Clercq,Yan Wang,Chris Carrie,Lin Xu,Beata Kmiec,Hayden Walker,Frank Van Breusegem,James Whelan,James Whelan,Estelle Giraud +14 more
TLDR
This work identifies a biological role for ANAC017 as an integral cellular component in mitochondrial retrograde signaling and a high-level transcriptional regulator that is necessary for H2O2-mediated primary stress responses in plants.Abstract:
Plants require daily coordinated regulation of energy metabolism for optimal growth and survival and therefore need to integrate cellular responses with both mitochondrial and plastid retrograde signaling. Using a forward genetic screen to characterize regulators of alternative oxidase1a (rao) mutants, we identified RAO2/Arabidopsis NAC domain-containing protein17 (ANAC017) as a direct positive regulator of AOX1a. RAO2/ANAC017 is targeted to connections and junctions in the endoplasmic reticulum (ER) and F-actin via a C-terminal transmembrane (TM) domain. A consensus rhomboid protease cleavage site is present in ANAC017 just prior to the predicted TM domain. Furthermore, addition of the rhomboid protease inhibitor N-p-Tosyl-l-Phe chloromethyl abolishes the induction of AOX1a upon antimycin A treatment. Simultaneous fluorescent tagging of ANAC017 with N-terminal red fluorescent protein (RFP) and C-terminal green fluorescent protein (GFP) revealed that the N-terminal RFP domain migrated into the nucleus, while the C-terminal GFP tag remained in the ER. Genome-wide analysis of the transcriptional network regulated by RAO2/ANAC017 under stress treatment revealed that RAO2/ANAC017 function was necessary for >85% of the changes observed as a primary response to cytosolic hydrogen peroxide (H2O2), but only ~33% of transcriptional changes observed in response to antimycin A treatment. Plants with mutated rao2/anac017 were more stress sensitive, whereas a gain-of-function mutation resulted in plants that had lower cellular levels of H2O2 under untreated conditions.read more
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Friend or foe? Reactive oxygen species production, scavenging and signaling in plant response to environmental stresses.
TL;DR: Over the past two decades it has been proven that ROS together with non‐photochemical quenching (NPQ), hormones, Ca2+ waves, and electrical signals are the main players in SAA and SAR, two physiological processes essential for plant survival and productivity in unfavorable conditions.
Journal ArticleDOI
Spreading the news: subcellular and organellar reactive oxygen species production and signalling
Lorin F. Mignolet-Spruyt,Enjun Xu,Niina Idänheimo,Frank A. Hoeberichts,Per Mühlenbock,Mikael Brosché,Frank Van Breusegem,Jaakko Kangasjärvi,Jaakko Kangasjärvi +8 more
TL;DR: Characterization of this comprehensive ROS signalling matrix may result in the identification of new targets and key regulators of ROS signalling, which might be excellent candidates for engineering or breeding stress-tolerant plants.
Journal ArticleDOI
The Roles of Mitochondrial Reactive Oxygen Species in Cellular Signaling and Stress Response in Plants
TL;DR: The shortcomings of the black box-idea of mtROS are discussed in the context of mechanistic considerations and the measurement ofmtROS, and directions for future research are provided, along with suggestions to increase reliability of MtROS measurements.
Journal ArticleDOI
The Membrane-Bound NAC Transcription Factor ANAC013 Functions in Mitochondrial Retrograde Regulation of the Oxidative Stress Response in Arabidopsis
Inge De Clercq,Vanessa Vermeirssen,Olivier Van Aken,Olivier Van Aken,Klaas Vandepoele,Monika W. Murcha,Simon R. Law,Annelies Inzé,Sophia Ng,Aneta Ivanova,Debbie Rombaut,Brigitte van de Cotte,Pinja Jaspers,Yves Van de Peer,Jaakko Kangasjärvi,James Whelan,James Whelan,Frank Van Breusegem +17 more
TL;DR: It is demonstrated that ANAC013 mediates MRR-induced expression of the MDS genes by direct interaction with the MDM cis-regulatory element and triggers increased oxidative stress tolerance and is characterized as a regulator of MRR upon stress in Arabidopsis thaliana.
Journal ArticleDOI
Interactions between cytokinin signalling and abiotic stress responses
Paul J. Zwack,Aaron M. Rashotte +1 more
TL;DR: What this body of research collectively implies with regard to cross-talk between cytokinin and abiotic stress tolerance is discussed, including the role of specific cytokinIn signalling components in a variety of stress responses.
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